SEARCH FOR GENES ASOCIATED WITH ORGAN SPECIFIC METASTASIS OF LUNG CANCER

患有肺癌的患者的症状仍然是贫穷的，其对Metastasis的影响。因此，它高度依赖于Metastasis的存在或缺失，以及对治疗的有效性。However，肺癌中Metastasis的分子机制仍然存在。我们建立了高metastatic sublines，来自人类肺腺癌细胞线，PC 14， LCMS通过体内选择。与父母细胞进行比较，这些子线在不同的器官中开发了明显的更多的metastatic nodules，如肾上腺素、骨头、淋巴节点，在注射后注入2 - 106个细胞进入裸米的尾带。在高metastatic sublines和parental cell之间没有显著的差异。在补充中，在高metastatic sublines和parental cell之间没有明显的差异。对36， 300克表达式的比较 ... More 由cDNA微阵列揭示的28个基因在父母PC 14细胞和高metastatic PC 14广告细胞之间有所不同的表达。在这些中，四种基因的表达（HRB 2、HS 3ST 3A 1、RAB 7、EDG-1）通过定量实时RT-PCR分析了人类肺癌和正常肺癌的组织specimen。三个基因的表达在正常的肺部比在癌症中更高。一个基因的表达比正常的肺部低得多。两个基因在表达中的显著差异，建议这两个基因可能是人体肺癌发生率和进展的关键决定者。该组织标本中四个基因的表达也通过定量实时RT-PCR分析比较了PC 14 Ad小鼠模型的元节点和亚切割节点之间的表达。高金属PC 14广告细胞中的金属节点和亚切割节点之间没有区别，建议对细胞线中这些基因的构造表达式。These cell lines may provide a useful model for studying mechanisms of metastasis of human lung cancer. Less（低）

The prognosis of patients with lung cancer is still poor due to its propensity for metastasis. Thus, it is highly dependent on the presence or absence of metastasis and on the effectiveness of treatment against it. However, molecular mechanisms of metastasis in lung cancer are still obscure. We established highly metastatic sublines, from the human lung adenocarcinoma cell lines, PC14, LCMS by in vivo selection. Compared to parental cells, these sublines developed significantly more metastatic nodules in the various organs, such as adrenal glands, bones, lymph nodes, lung after injection of 2 エ 106 cells into the tail vein of nude mice. There were no significant differences in the subcutaneous tumorigenicity in vivo and in the growth rates in vitro between high-metastatic sublines and parental cells. In addition, there were no significant differences in motility, invasiveness, and adherence between high-metastatic sublines and parental cells. A comparison of the expression of 36, 300 g … More enes by cDNA microarray revealed that 28 genes were differentially expressed between parental PC14 cells and high-metastatic PC14Ad cells. Among these, expression of four genes (HRB2, HS3ST3A1, RAB7, EDG-1) in the tissue specimen of human lung cancer and adjacent normal lung were analysed by quantitative real-time RT-PCR analysis. Expression of 3 genes was higher in the cancer tissue than in the normal lung. Expression of 1 genes was lower in the cancer tissue than in the normal lung. The differences in expression were significant in the two genes, suggesting that these two genes might be a key determinant of carcinogenesis and progression of human lung cancer. Expression of four genes in the tissue specimen was also compared between metastatic nodules and subcutaneous nodule of PC14Ad mouse models by quantitative real-time RT-PCR analysis. There were no differences between metastatic nodules and subcutaneous nodule in the high-metastatic PC14Ad cells, suggesting constitutive expression of these genes in the cell line. These cell lines may provide a useful model for studying mechanisms of metastasis of human lung cancer. Less