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Effect of different anesthetics and anesthetic exposure times on survival course of sepsis-analysis of mRNA in mice performed cecal ligation & puncture-

不同麻醉剂和麻醉剂暴露时间对盲肠结扎小鼠脓毒症生存过程的影响-mRNA分析

基本信息

批准号：
08457401
负责人：
IMAI Takasuke
金额：
$ 4.35万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

PURPOSE : To determine whether the survival curves and rates differ between a mutant strain of mice (C3H/HeJ : endotoxin-resistant) and endotoxin-susceptible strain (C3H/HeN) when severe sepsis (cecal ligation and puncture : CLP) is performed with different anesthetics (ketamine or varied exposure to halothane).METHODS : CLP was performed under a randomly chosen anesthetic method out of inhalation of halothane in oxygen (15 min, 2 hr, or 6 hr) (1.00,120, and 103 mice respectively) or a single injection of ketamine im (127 mice) in paired fashion in 8-week-old male mice of each strain. The daily survival rates were compared between the two strains until the 10th day after CLP for each anesthetic and among the four anesthetic methods for each strain using multiple comparisons.RESULTS : The C3H/HeJ had delayd death relative to the C3H/HeN with every anrsthetic method except a two hour halothane (p<0.01 at day 1 and day 2-5 in halothane 15 min and 6 hr, p<0.05 at day 4-6 in ketamine), however, the final survival rates for each method of anesthesia were the same in the two strains. Regardless of the genetic susceptibility to endotoxin, short exposure to halothane resulted to the most rapid death compared with the other anesthetic methods (p<0.05>, and a two hour halothane showed the best survival.CONCLUSIONS : The endotoxin susceptibility affected the septic course for each anesthetic method, and the anesthetic methods influenced the survival from sepsis in both strains.

目的：确定当使用不同麻醉剂（氯胺酮或不同的氟烷暴露量）进行严重脓毒症（盲肠结扎和穿刺：CLP）时，突变型小鼠品系（C3H/HeJ：内毒素抗性）和内毒素敏感品系（C3H/HeN）的生存曲线和存活率是否存在差异。方法：CLP 在随机选择的条件下进行麻醉方法为吸入氧气中的氟烷（15 分钟、2 小时或 6 小时）（分别为 1.00、120 和 103 只小鼠）或以配对方式在每个品系的 8 周龄雄性小鼠中单次注射氯胺酮（127 只小鼠）。使用多重比较，比较两种毒株之间直至 CLP 后第 10 天的每日存活率，以及每种毒株的四种麻醉方法之间的每日存活率。结果：相对于 C3H/HeN，除了两小时氟烷外，C3H/HeJ 相对于 C3H/HeN 的每种麻醉方法均延迟死亡（在氟烷中 15 分钟和 6 小时，第 1 天和第 2-5 天的 p<0.01，第 4-6 天 p<0.05 然而，每种麻醉方法的最终存活率在两种菌株中是相同的。无论对内毒素的遗传敏感性如何，与其他麻醉方法相比，短时间暴露于氟烷会导致最快的死亡（p<0.05>，并且两小时氟烷显示出最佳的存活率。结论：内毒素敏感性影响每种麻醉方法的败血症病程，并且麻醉方法影响两种菌株的败血症存活率。