STUDY ON CHARACTERISTICS OF TUMOR CELLS AND MICRO ENVIROMENTAL FACTORS OF HIGHLY INVASIVE ORAL SQUAMOUS CELL CARCINOMA

高侵袭性口腔鳞状细胞癌肿瘤细胞特征及微环境因素研究

• 批准号: 08457553
• 负责人: NOGUCHI Makoto
• 金额: $ 4.42万
• 依托单位: SAPPORO MEDICAL UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457553/
• 关键词: ORAL CANCER; CELLULAR DNA CONTENTS; TYPE I COLLAGEN; VEGF; GLUT-1; 舌扁平上皮癌由来細胞株; Glucose transpoter-1; VEGF isotype; 内皮細胞; 細胞間透過性; 口腔扁平上皮癌; 癌浸潤様式; 核DNA量; 7型コラーゲン; 血管新生因子; 超4C率; FactorVIII; CD34

1. Cellular DNA Contents(1) Cytometric analysis was useful in detecting DNA aneuploid cells, especially when small numbers of cancer cells intermingled with stromal cells.(2) Prognostic value of malignancy grading based on mode of cancer invasion and rate of cells exceeding 4C DNA contents (4CER) was suggested.2. Interstitial Collagen Deposition(1) Immunohistochemical localization of type VII collagen at the invading edge of tumors may reflect the histological malignancy of oral squaous cell carcinoma(oral SCC).(2) Immunostaining pattern for type I collagen of oral SCC can provide information of importance in determining safe margins.3. Influence to Barrier Function of HUVEC HUVEC added conditioning medium of more invasive oral SCC cell line showed higher transcellular electrical resistance than other oral SCC cell line with lower invasive potential.4. Expression of VEGF/VPF and GLUT-1(1) Expression of GLUT-1 was not found in OSC 30. In OSC 70, GLUT-1 expressed intensively in their cytoplasm.(2) Although the expression of VEGF were detected in both cell lines, the staining patters were different between OSC3O and OSC7O.(3) VEGF isotypes was similar between both cell lines.

1.细胞DNA含量（1）细胞计数法可用于检测DNA异倍体细胞，特别是当少量癌细胞与间质细胞混合时。(2)结论：1.根据肿瘤浸润方式和细胞DNA含量超过4C的比率（4CER）进行恶性程度分级对预后的价值.间质胶原沉积（1）VII型胶原在肿瘤浸润边缘的免疫组化定位可反映口腔鳞状细胞癌的组织学恶性程度。(2)I型胶原的免疫组化染色模式可为口腔鳞癌安全范围的确定提供重要信息.对HUVEC屏障功能的影响加入条件培养液的侵袭性较强的口腔鳞癌细胞系HUVEC的跨细胞电阻高于侵袭性较低的其他口腔鳞癌细胞系. VEGF/VPF和GLUT-1的表达（1）在OSC 30中未发现GLUT-1的表达。在OSC 70中，GLUT-1在其细胞质中强烈表达。(2)两种细胞系均表达VEGF，但OSC 30和OSC 70的表达模式不同。(3)两种细胞系之间VEGF同种型相似。

项目成果

TAKASHI SEKIGUCHI st al.: "Clinical significance of interstitial collagen deposition at the invading edge in oral cancer : immunohistochemistry for Type I collagen." Int.J.Clin.Onc.Vol.2. 21-28 (1997)

TAKASHI SEKIGUCHI 等人：“口腔癌侵袭边缘间质胶原蛋白沉积的临床意义：I 型胶原蛋白的免疫组织化学。”

MAKOTO NOGUCHI et al.: "Malignancy grading based on mode of invasion and nuclear DNA contents for oral squamous cell carcinomas" Head and Neck Cancer. 23 (3). 691-697 (1997)

MAKOTO NOGUCHI 等人：“基于口腔鳞状细胞癌的侵袭模式和核 DNA 含量的恶性肿瘤分级”头颈癌。

野口 誠: "高度浸潤性口腔癌の臨床病態像" 日本口腔科学会雑誌. 45・2. 184-189 (1996)

野口诚：“高侵袭性口腔癌的临床病理学”日本口腔医学会杂志 45・2（1996）。

仲盛健治: "口腔扁平上皮癌における細胞核DNA量解析" 日口外誌. 44. 617-624 (1998)

Kenji Nakamori：“口腔鳞状细胞癌中核 DNA 含量的分析”Nichigu Gaishi 44. 617-624 (1998)。

関口 隆: "口腔扁平上皮癌の発育先進部におけるVII型コラーゲンの局在" 日本口腔外科学会雑誌. 42・2. 147-154 (1996)

Takashi Sekiguchi：“口腔鳞状细胞癌晚期生长区域中的 VII 型胶原的定位”日本口腔颌面外科杂志 42・2（1996）。