STUDY ON CHARACTERISTICS OF TUMOR CELLS AND MICRO ENVIROMENTAL FACTORS OF HIGHLY INVASIVE ORAL SQUAMOUS CELL CARCINOMA

1. 细胞DNA含量(1)细胞分析对于检测DNA非整倍体细胞是有用的，特别是当少量癌细胞与基质细胞混杂时。(2)提出了基于肿瘤侵袭方式和超过4C DNA含量（4CER）的细胞率的恶性分级的预后价值。(1)肿瘤侵袭边缘的VII型胶原的免疫组化定位可能反映口腔鳞状细胞癌（oral squaous cell carcinoma， SCC）的组织学恶性程度。(2)口腔鳞状细胞癌I型胶原的免疫染色模式可以为确定安全界限提供重要信息。3 . HUVEC加入调节培养基对侵袭性强的口腔鳞状细胞癌细胞株的跨细胞电阻高于其他侵袭电位较低的口腔鳞状细胞癌细胞株。VEGF/VPF和GLUT-1的表达(1)GLUT-1在OSC 30中未见表达。在osc70中，GLUT-1在细胞质中密集表达。(2)虽然在两种细胞系中均检测到VEGF的表达，但osc30和osc70的染色模式不同。(3) VEGF同型在两种细胞系间相似。

1. Cellular DNA Contents(1) Cytometric analysis was useful in detecting DNA aneuploid cells, especially when small numbers of cancer cells intermingled with stromal cells.(2) Prognostic value of malignancy grading based on mode of cancer invasion and rate of cells exceeding 4C DNA contents (4CER) was suggested.2. Interstitial Collagen Deposition(1) Immunohistochemical localization of type VII collagen at the invading edge of tumors may reflect the histological malignancy of oral squaous cell carcinoma(oral SCC).(2) Immunostaining pattern for type I collagen of oral SCC can provide information of importance in determining safe margins.3. Influence to Barrier Function of HUVEC HUVEC added conditioning medium of more invasive oral SCC cell line showed higher transcellular electrical resistance than other oral SCC cell line with lower invasive potential.4. Expression of VEGF/VPF and GLUT-1(1) Expression of GLUT-1 was not found in OSC 30. In OSC 70, GLUT-1 expressed intensively in their cytoplasm.(2) Although the expression of VEGF were detected in both cell lines, the staining patters were different between OSC3O and OSC7O.(3) VEGF isotypes was similar between both cell lines.