STUDY ON CHARACTERISTICS OF TUMOR CELLS AND MICRO ENVIROMENTAL FACTORS OF HIGHLY INVASIVE ORAL SQUAMOUS CELL CARCINOMA

1.细胞DNA含量(1)细胞仪检出DNA异倍体细胞，尤其是少数癌细胞与间质细胞混杂时。(2)提出了以癌细胞侵袭方式和4C DNA含量以上细胞比率(4CER)为依据的恶性程度分级的预后价值。间质胶原沉积(1)免疫组织化学定位于肿瘤侵袭边缘，可反映口腔鳞癌的组织学恶性程度。(2)口腔鳞癌组织中I型胶原的免疫组织化学染色模式可为确定安全边界提供重要信息。对HUVEC屏障功能的影响HUVEC加入侵袭能力较强的口腔鳞癌细胞系的条件培养液，其跨细胞电阻高于其他侵袭潜能较低的口腔鳞癌细胞系。VEGF/VPF和GLUT-1的表达(1)OSC 30未见GLUT-1的表达。OSC70中GLUT-1在胞浆中表达较强。(2)OSC30和OSC70中均可检测到VEGF的表达，但染色模式不同。

1. Cellular DNA Contents(1) Cytometric analysis was useful in detecting DNA aneuploid cells, especially when small numbers of cancer cells intermingled with stromal cells.(2) Prognostic value of malignancy grading based on mode of cancer invasion and rate of cells exceeding 4C DNA contents (4CER) was suggested.2. Interstitial Collagen Deposition(1) Immunohistochemical localization of type VII collagen at the invading edge of tumors may reflect the histological malignancy of oral squaous cell carcinoma(oral SCC).(2) Immunostaining pattern for type I collagen of oral SCC can provide information of importance in determining safe margins.3. Influence to Barrier Function of HUVEC HUVEC added conditioning medium of more invasive oral SCC cell line showed higher transcellular electrical resistance than other oral SCC cell line with lower invasive potential.4. Expression of VEGF/VPF and GLUT-1(1) Expression of GLUT-1 was not found in OSC 30. In OSC 70, GLUT-1 expressed intensively in their cytoplasm.(2) Although the expression of VEGF were detected in both cell lines, the staining patters were different between OSC3O and OSC7O.(3) VEGF isotypes was similar between both cell lines.