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Biological functions of novel human plasma proteins, IHRP and PHBP.

新型人血浆蛋白 IHRP 和 PHBP 的生物学功能。

基本信息

批准号：
08457615
负责人：
TOMITA Motowo
金额：
$ 5.06万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

We tried to elucidate biological functions of novel human plasma proteins, IHRP and PHBP, discovered by us in 1994 and 1995, respectively.1. Study on JHRP(1) Protein structure : human JHRP is a single chain polypeptide of about 900 amino acids. Porcine and mouse IHRP cDNAs were cloned, the amino acid sequence homology was about 70% among all the three species. All IHRPs obtained from the three animal species had a site susceptible to proteases. (2) Genomic structure : human IHRP genome was composed of 24 exons, and homologous to that of the heavy chain of inter-aipha-trypsin inhibitor. (3) Functional analysis : although bovine IHRP has been reported to be a typical acute phase protein, the plasma concentration of humam IHRP did not inrease under inflammation conditions. In mice, IHRP was deposited in the kidney after the induction of inflammation and an annexin was involved in the deposition.2. Study on PHBP(1) Protein structure : human and mouse PHBP cDNAs were cloned. The two PHBPs showed a 70% homology in amino acid sequence. (2) Genomic structure : human PHBP genome was composed of 13 exons, and significantly similar to that of the coagulation factor XII.(3)functional analysis : PHBP was a serine protease that specically cleaved the peptide bond at C-terminal side of Arg residue. PHBP is a single chain polypeptide that shows no proteolytic activity under normal conditions, and is activated by the cleavage at a specific position.

我们试图阐明我们分别于1994年和1995年发现的新型人类血浆蛋白IHRP和PHBP的生物学功能。1． JHRP的研究 (1)蛋白质结构：人JHRP是约900个氨基酸的单链多肽。克隆了猪和小鼠的IHRP cDNA，三个物种的氨基酸序列同源性约为70%。从这三种动物中获得的所有 IHRP 都有一个对蛋白酶敏感的位点。 (2)基因组结构：人IHRP基因组由24个外显子组成，与间α-胰蛋白酶抑制剂的重链同源。 (3)功能分析：尽管据报道牛IHRP是典型的急性时相蛋白，但在炎症条件下，humam IHRP的血浆浓度并不增加。小鼠炎症诱导后，IHRP在肾脏沉积，其中膜联蛋白参与沉积。 2． PHBP的研究(1)蛋白质结构：克隆了人和小鼠的PHBP cDNA。两种PHBP在氨基酸序列上显示出70%的同源性。 (2)基因组结构：人类PHBP基因组由13个外显子组成，与凝血因子XII显着相似。(3)功能分析：PHBP是一种丝氨酸蛋白酶，特异性裂解Arg残基C端的肽键。 PHBP是一种单链多肽，在正常条件下不显示蛋白水解活性，并通过特定位置的裂解而被激活。