Expression and Function of IHRP

IHRP 的表达和功能

• 批准号: 09672250
• 负责人: TOBE Takashi
• 金额: $ 1.98万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672250/
• 关键词: IHRP,; inflammation,; Turpentine oil,; LPS; デキサメサゾン; 急性期蛋白質; STAT3; アネキシン

The mouse counterpart of human IHRP has been partially purified from mouse serum, and the cDNA come encoding this protein was isolated and characterized. The amino acid sequence of mouse IHRP predicted from the nucleotide sequence of cDNA shows reasonable homology to those of human and pig IHRP.The N-terminal amino acid sequence of partially purified mouse IHRP was completely matched to that predicted from its cDNA.The expression of IHRP in mouse was induced by treatment with turpentine oil or LPS.The induction of IHRP by turpentine was effectively prevented by pretreatment with dexamethasone, but the induction by LPS was not- affected by dexamethasone.

从小鼠血清中部分纯化了人IHRP的小鼠对应蛋白，并分离和鉴定了编码该蛋白的cDNAComm。预测的小鼠IHRP的氨基酸序列与人和猪IHRP的氨基酸序列具有较好的同源性，部分纯化的小鼠IHRP的N端氨基酸序列与其cDNA预测的氨基酸序列完全匹配。松节油或LPS可诱导小鼠IHRP的表达，地塞米松可有效地阻止松节油诱导的IHRP表达，但不影响脂多糖的诱导作用。

项目成果

Jun-ichi Sumiya: "Isolation and Characterization of the Plasma Hyaluronan-Binding Protein (PHBP) Gene" J.Biochem.122. 983-990 (1997)

Jun-ichi Sumiya：“血浆乙酰透明质酸结合蛋白 (PHBP) 基因的分离和表征”J.Biochem.122。

Ken Hashimoto: "Cloning of the cDNA for a mouse homologue of human PHBP:a Novel hyaluronan-binding protein." Biol.Pharm.Bull.20・11. 1127-1130 (1997)

Ken Hashimoto：“克隆人 PHBP 的小鼠同源物：一种新型透明质酸结合蛋白。Biol.Pharm.Bull.20·11（1997）。”