Molecular Mechansims for Cellular Responses to Radiation and Oxidative Stresses

细胞对辐射和氧化应激反应的分子机制

• 批准号: 08458153
• 负责人: YONEI Shuji
• 金额: $ 4.35万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458153/
• 关键词: Glutathione; Thioredoxin; Active Oxygen Species; Redox Regulation; OxyR; Diamide; SH Oxidation; グルタレドキシン; レドックス; 過酸化水素; X線感受性; 酸化ストレス応答; 宿主細胞回復; DNA修復; 酸化的DNA損傷; oxyR; レギュロン

We examined the pathways that thioredoxin and thioredoxin reductase protect Escherichia coli cells against hydrogen peroxide. In stationary phase, the reduced form of thioredoxin protected cells by scavenging reactive oxygen species. On the other hand, in early growth phase, the oxidized form of thioredoxin exerted as a regulatory factor for theinduction of adaptive response genes such as katG that encodes catalase- hydroperoxidase I.These effects of thioredoxin systems were mediated by the OxyR protein. In addition, the expression of the katG gene was induced by diamide under anaerobic conditions, suggesting that a disruption of redox balance results in the induction of OxyR regulon without hydrogen peroxide. Therefore, the redox state of thioredoxin is a critical factor for the redox stress response in E.coli. We examined the regulation of thioredoxin and thioredoxin reductase expression.

我们研究了硫氧还蛋白和硫氧还蛋白还原酶保护大肠杆菌细胞对抗过氧化氢的途径。在固定阶段，硫氧还蛋白的还原形式通过清除活性氧来保护细胞。另一方面，在生长早期，硫氧还蛋白的氧化形式作为一种调节因子，用于诱导编码过氧化氢酶-过氧化物酶i的适应性反应基因，如katG，这些硫氧还蛋白系统的作用是由OxyR蛋白介导的。此外，在厌氧条件下，二胺诱导了katG基因的表达，这表明氧化还原平衡的破坏导致了在没有过氧化氢的情况下诱导了OxyR调控。因此，硫氧还蛋白的氧化还原状态是大肠杆菌氧化还原应激反应的关键因素。我们检测了硫氧还蛋白和硫氧还蛋白还原酶的表达调控。

项目成果

K.Yamamoto,F.Uraki,S.Yonei and O.Yukawa: "Enzymatic repair mechanisms for base modifications induced by oxygen radicals" J.Radiat.Res.(印刷中). (1997)

K. Yamamoto、F. Uraki、S. Yonei 和 O. Yukawa：“氧自由基诱导的碱基修饰的酶修复机制”J. Radiat（出版中）。

H.Sugiyama,S.Matsuda,Q.M.Zhang,S.Yonei and I.Saito: "New sythetic method of 5 formyluracil containing oligonucleotides and their melting behavior" Tetrahedron Lett.37・50. 9067‐9070 (1996)

H.Sugiyama、S.Matsuda、Q.M.Zhang、S.Yonei 和 I.Saito：“含 5 甲酰尿嘧啶寡核苷酸的新合成方法及其熔化行为”Tetrahedron Lett.37・50 (1996)。

Yonei, S., Q.-M.ZHANG,Y.MATSUMOTO,T.NAKAHARA: "Oxidative Stress and Cellular Responses : Biological Paradox (Japanese)" Radiation Biology Research Communications. 32. 87-104 (1997)

Yonei, S., Q.-M.ZHANG,Y.MATSUMOTO,T.NAKAHARA：“氧化应激和细胞反应：生物悖论（日语）”辐射生物学研究通讯。

Q.M.Zhang and S.Yonei: "Replication of DNA templates containing 5-formyluracil,a major oxidative lesion of thymine in DNA" Nucleic Acids Research. 25(20). 3969-3974 (1997)

Q.M.Zhang 和 S.Yonei：“含有 5-甲酰尿嘧啶的 DNA 模板的复制，这是 DNA 中胸腺嘧啶的主要氧化损伤”，《核酸研究》。

Zhang, Q.M., A.Tachibana and S.Yonei: Oxygen stress model : E.coli, cultured cells in : Experimental Protocols for reactive oxygen species and reactive nitrogen species (RNS) (Edited by N.Taniguchi and J.M.C.Gutteridge). Oxford University Press, (in press

张，Q.M.，A.Tachibana 和 S.Yonei：氧应激模型：大肠杆菌，培养细胞：活性氧和活性氮 (RNS) 的实验方案（由 N.Taniguchi 和 J.M.C.Gutteridge 编辑）。