The role of kinesin neck region in force generation

驱动蛋白颈部区域在力产生中的作用

• 批准号: 08458204
• 负责人: TOYOSHIMA Yoko
• 金额: $ 4.61万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458204/
• 关键词: motor proteins; microtubules; kinesin; ncd; motor domain; neck region; polarity of the movement; 頸部; ミオシン; 回転自由度

Ncd, a member of kinesin super family, moves toward the minus end of microtubule. Neck region of kineshin and ncd is considered to be important to determine the polarity of the movement, because the structural difference between the motor domains of kineshin and ncd is not essential. We made a chymeric protein which consists of kinesin motor domain and ncd neck domain. This chymeric protein did not show the directional movement on microtubules. The measurement of ATPase activity revealed that the values of Kcat and K_<50%MT> are 7.8 S^<-1> and 0.22 muM,respectively. Comparing these values with those of kineshin fragment (kineshin motor domain and kineshin neck domain), the chymeric protein seemed to be unable to perform the processive movement. These results indicate that the neck region play an important role to determine the polarity of the movement.

NCD是动蛋白超家族的一员，它向微管的负端运动。动蛋白和NCD的颈部区域被认为是决定运动极性的重要因素，因为动蛋白和NCD的运动域之间的结构差异并不重要。我们制备了一种由运动蛋白运动区和NCD颈区组成的融合蛋白。该融合蛋白在微管上没有显示出定向运动。ATPase活性测定表明，Kcat和K_(50)MT&Gt；分别为7.8um和0.22um。与Kineshin片段(Kineshin运动区和Kineshin Neck区)的这些值比较，似乎不能进行前进运动。这些结果表明，颈部区域在决定运动的极性方面起着重要作用。

项目成果

Lnoue,Y., Toyoshima,Y.Y., Iwane,A.H., Morimoto,S., Higuchi,H., Yanagida,T.: "Movements of truncated kinesin fragments with a short or an artificial flexible neck" Proc.Natl.Acad.Sci.USA. 94. 7275-7280 (1997)

Lnoue，Y.，Toyoshima，Y.Y.，Iwane，A.H.，Morimoto，S.，Higuchi，H.，Yanagida，T.：“短或人工柔性颈部截断的驱动蛋白片段的运动”Proc.Natl.Acad.Sci

Inoue, Y., Toyoshima, Y.Y., Iwane, A.H., Morimoto, S., Higuchi, H., & Yanagida, T.: "Movements of truncated kinesin fragments with a short or an artificial flexible neck" Proc.Natl.Acad.Sci.USA. 94. 7275-7280 (1997)

井上 Y.、丰岛 Y.Y.、岩根 A.H.、森本 S.、樋口 H.、

Imafuku,Y.,Toyoshima,Y.& Tawada,K.: "Fluctuation in the microtubule sliding movement drinven by kinesin in vitro." Biophys.J.70. 878-886 (1996)

今福，Y.，丰岛，Y.

豊島 陽子: "微小管系モーターの多様な運動"

丰岛洋子：“微管马达的多样化运动”

Imafuku,Y.,Toyoshima,Y.& Tawada,K.: "A Monte Carlo study for a fluctuation analysis in the in vitro motility by protein motors." Biophys.Chem.59. 139-153 (1996)

今福，Y.，丰岛，Y.