Higher Order Structure of Long DNA Molecules : Controlling the Structure Based on the Knoledge of the Mechanism in the Transition
Higher Order Structure of Long DNA Molecules : Controlling the Structure Based on the Knoledge of the Mechanism in the Transition
批准号:
08459013
负责人:
YOSHIKAWA Ken-ichi
金额:
$4.22万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1996
资助国家:
日本
项目状态:
已结题
起止时间:
1996 至 1997
中文摘要
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英文摘要
In the present research, we have fownd that individual single duplex DNA chains undergoes large discrete phase transition between elongated coil and compacted globule (coil-globule transition), based on the single chain observation by use of fluorescence microscopy.Whereas ensemble of DNA has the characteristics of diffuse or continuous transition. From single chain observation, we found that the first order phase transition is induced by various kinds of condensation agents. As for as we know, this is the first finding on "nucleation & growth" from a single polymer, not only DNA but also all the other synthetic and natural polymers. In addition to this, the relationship between gene-transfection efficency and higher-order structure of the DNA carrier-complex was discussed.
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S.G.Starodoubtsev: "Intrachain Setregation in Single Giant DNA Molecules Induced by Poly (2-vinylpyrrolidone)" J.Phys.Chem.100. 19702-19705 (1996)
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N.Emi: ""Gene Transfer Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate"" Biochem.Biophys.Res.Commum.Vol.231. 421-424 (1997)
N.Emi:“聚精氨酸介导的基因转移需要形成 DNA 聚集体的大载体复合物”Biochem.Biophys.Res.Commum.Vol.231。
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N.Emi: "Gene Transfer Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate" Biochem.Biophys.Res.Commun.231. 421-424 (1997)
N.Emi:“聚精氨酸介导的基因转移需要形成 DNA 聚集体的大载体复合物”Biochem.Biophys.Res.Commun.231。
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V.V.Vasilevskaya: "Structure of Collapsed Persistent Macromolecule : Toroids vs. Spherical Globule" Biopolymers. 41. 51-60 (1997)
V.V.Vasilevskaya:“塌陷持久性大分子的结构:环形与球状小球”生物聚合物。
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共 52 条
Analytic Torsion and Automorphic Forms with Infinite Products
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批准号:12640061
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.3万
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财政年份:2000
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负责人:YOSHIKAWA Ken-ichi
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依托单位:
海外基金