Higher Order Structure of Long DNA Molecules : Controlling the Structure Based on the Knoledge of the Mechanism in the Transition

长DNA分子的高阶结构:基于转变机制的知识控制结构

• 批准号: 08459013
• 负责人: YOSHIKAWA Ken-ichi
• 金额: $ 4.22万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08459013/
• 关键词: Long DNA Chain; First Order Phase Transition; Single Chain Observation; Fruorescence Microscopy; Gene Transfection; Phase Separation in a Single Molecule; Coil-Globule Transition; Monte Carle Simulation; 履歴現象

In the present research, we have fownd that individual single duplex DNA chains undergoes large discrete phase transition between elongated coil and compacted globule (coil-globule transition), based on the single chain observation by use of fluorescence microscopy.Whereas ensemble of DNA has the characteristics of diffuse or continuous transition. From single chain observation, we found that the first order phase transition is induced by various kinds of condensation agents. As for as we know, this is the first finding on "nucleation & growth" from a single polymer, not only DNA but also all the other synthetic and natural polymers. In addition to this, the relationship between gene-transfection efficency and higher-order structure of the DNA carrier-complex was discussed.

本文通过荧光显微镜对单链的观察，发现单个双链DNA链在伸长的螺旋和紧密的小球之间发生了大的离散相变（螺旋-小球相变），而DNA的整体则具有扩散或连续相变的特征。从单链的观察，我们发现一级相变是由各种缩合剂引起的。据我们所知，这是第一次从单一聚合物中发现“成核和生长”，不仅是DNA，而且是所有其他合成和天然聚合物。此外，还讨论了DNA载体复合物的高级结构与基因转染效率的关系。

项目成果

Y.Yoshikawa: "Folding and Aggregation of DNA Chains Induced by Complexation with Lipospermine : Formation of a Nuclesome-Like Structure and Network Assembly" FEBS Lett.396. 71-76 (1996)

Y.Yoshikawa：“脂精胺络合诱导的 DNA 链折叠和聚集：类核体结构和网络组装的形成”FEBS Lett.396。

S.G.Starodoubtsev: "Intrachain Setregation in Single Giant DNA Molecules Induced by Poly (2-vinylpyrrolidone)" J.Phys.Chem.100. 19702-19705 (1996)

S.G.Starodoubtsev：“聚 (2-乙烯基吡咯烷酮) 诱导的单个巨型 DNA 分子的链内调整”J.Phys.Chem.100。

N.Emi: ""Gene Transfer Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate"" Biochem.Biophys.Res.Commum.Vol.231. 421-424 (1997)

N.Emi：“聚精氨酸介导的基因转移需要形成 DNA 聚集体的大载体复合物”Biochem.Biophys.Res.Commum.Vol.231。

N.Emi: "Gene Transfer Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate" Biochem.Biophys.Res.Commun.231. 421-424 (1997)

N.Emi：“聚精氨酸介导的基因转移需要形成 DNA 聚集体的大载体复合物”Biochem.Biophys.Res.Commun.231。

V.V.Vasilevskaya: "Structure of Collapsed Persistent Macromolecule : Toroids vs. Spherical Globule" Biopolymers. 41. 51-60 (1997)

V.V.Vasilevskaya：“塌陷持久性大分子的结构：环形与球状小球”生物聚合物。