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Higher Order Structure of Long DNA Molecules : Controlling the Structure Based on the Knoledge of the Mechanism in the Transition

Higher Order Structure of Long DNA Molecules : Controlling the Structure Based on the Knoledge of the Mechanism in the Transition
长DNA分子的高阶结构:基于转变机制的知识控制结构
批准号:
08459013
负责人:
YOSHIKAWA Ken-ichi
金额:
$4.22万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
1996
资助国家:
日本
项目状态:
已结题
起止时间:
1996 至 1997

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中文摘要
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英文摘要
In the present research, we have fownd that individual single duplex DNA chains undergoes large discrete phase transition between elongated coil and compacted globule (coil-globule transition), based on the single chain observation by use of fluorescence microscopy.Whereas ensemble of DNA has the characteristics of diffuse or continuous transition. From single chain observation, we found that the first order phase transition is induced by various kinds of condensation agents. As for as we know, this is the first finding on "nucleation & growth" from a single polymer, not only DNA but also all the other synthetic and natural polymers. In addition to this, the relationship between gene-transfection efficency and higher-order structure of the DNA carrier-complex was discussed.
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Y.Yoshikawa: "Folding and Aggregation of DNA Chains Induced by Complexation with Lipospermine : Formation of a Nuclesome-Like Structure and Network Assembly" FEBS Lett.396. 71-76 (1996)
Y.Yoshikawa:“脂精胺络合诱导的 DNA 链折叠和聚集:类核体结构和网络组装的形成”FEBS Lett.396。
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S.G.Starodoubtsev: "Intrachain Setregation in Single Giant DNA Molecules Induced by Poly (2-vinylpyrrolidone)" J.Phys.Chem.100. 19702-19705 (1996)
S.G.Starodoubtsev:“聚 (2-乙烯基吡咯烷酮) 诱导的单个巨型 DNA 分子的链内调整”J.Phys.Chem.100。
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N.Emi: ""Gene Transfer Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate"" Biochem.Biophys.Res.Commum.Vol.231. 421-424 (1997)
N.Emi:“聚精氨酸介导的基因转移需要形成 DNA 聚集体的大载体复合物”Biochem.Biophys.Res.Commum.Vol.231。
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通讯作者:
N.Emi: "Gene Transfer Mediated by Polyarginine Requires a Formation of Big Carrier-Complex of DNA Aggregate" Biochem.Biophys.Res.Commun.231. 421-424 (1997)
N.Emi:“聚精氨酸介导的基因转移需要形成 DNA 聚集体的大载体复合物”Biochem.Biophys.Res.Commun.231。
DOI: --
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52
    Analytic Torsion and Automorphic Forms with Infinite Products
    • 批准号:
      12640061
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.3万
    • 财政年份:
      2000
    • 负责人:
      YOSHIKAWA Ken-ichi
    • 依托单位:
    海外基金