Optical Ion Sensors Based on LB Films

基于 LB 薄膜的光学离子传感器

• 批准号: 08555209
• 负责人: SHIMOMURA Masatsugu
• 金额: $ 0.64万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08555209/
• 关键词: monolayrs; fluorescence; optical sensor; ion sensor; LB Films; bilayr membranes; polyion complex; nucleic acid; マルチチャンネル

In order to design a multi-channel sensing system based on pattern recognition, fluorescence response of bilayr membranes having octadecylrhodamine B to various inorganic anions were investigated. Fourteen cationic amphiphiles, double chain amphiphiles and single chain amphiphiles with a rigid aromatic segment, were used as the sensing bilayr membranes. Fluorescence response of bilayr membranes was strongly depend not only on inorganic anions but also on the chemical structure of the bilayr forming amphiphile. Fluid bilayr membranes were more sensitive than those of gel state. Iodide ion act as a fluorescence quencher of the most bilayr membranes. Carbonate ions specifically enhanced fluorescence intensity of the bilayr membrane of a single chain viologen amphiphile. Based on the results of the bilayr membranes fluorescence responses of Langmuir-Blodgett films of the cationic amphiphiles complexed with counter charged polyelectrolites, Poly(stirenesulfonate), poly(vinylsulfate), and carboxymethyl-cellulose, were investigated. The chemical structure of the counter charged polymer strongly affected the fluorescence response even if the same amphiphile was used as a monolayr forming compound. A large variety of the fluorescence of the molecular films is essential to the molecular design of the multi-channel ion sensors.

为了设计一种基于模式识别的多通道传感系统，研究了十八烷基罗丹明B双层膜对各种无机阴离子的荧光响应。14种阳离子两亲分子、双链两亲分子和带有刚性芳香链段的单链两亲分子被用作传感双层膜。双层膜的荧光响应不仅强烈地依赖于无机阴离子，而且还依赖于形成两亲分子的双分子膜的化学结构。液体双分子膜比凝胶双分子膜更敏感。碘离子在大多数双层膜中起到荧光猝灭的作用。碳酸盐离子特别增强了单链紫精两亲分子双层膜的荧光强度。根据双层膜的结果，研究了阳离子两亲分子与反电荷聚电解质、聚(苯乙烯磺酸)、聚(乙烯基硫酸酯)和羧甲基纤维素络合形成的Langmuir-Blodget膜的荧光响应。即使使用相同的两亲分子作为单分子形成化合物，反电荷聚合物的化学结构也会对荧光响应产生强烈的影响。分子膜的荧光种类繁多，对于多通道离子传感器的分子设计是必不可少的。

项目成果

M.Shimomura, et al.: "Optical Sensing of Molecular Recognition in Surface Monolayrs" Proteins, Nucleic Acids, and Enzymes (Japanese). 41. 83 (1996)

M.Shimomura 等人：“表面单层分子识别的光学传感”蛋白质、核酸和酶（日语）。

下村 政嗣、居城 邦治: "単分子膜による分子認識とオプティカルセンシング" 蛋白質核酸酵素. 41. 83-88 (1996)

Masatsugu Shimomura、Kuniharu Ishiro：“使用单分子层的分子识别和光学传感”蛋白质核酸酶。 41. 83-88 (1996)

F.Nakamura, M.Shimomura, 他: "Construction of two-dimensional DNA-mimetics.Supramolecular Architecture with Highly Stacked Base-Pairs of Amphiphilic Adenine and Thymine at the Air-Water Interface." Thin Solid Films. (発表予定). (1998)

F.Nakamura、M.Shimomura 等人：“二维 DNA 模拟物的构建。在空气-水界面具有高度堆积的两亲性腺嘌呤和胸腺嘧啶碱基对的超分子结构（即将成为固体薄膜）”提出）（1998）。

N.Maruyama, M.Shimomura, 他: "2-D Patterns of Molecular Aggregates on Solid Substrates" Thin Solid Films. (発表予定). (1998)

N.Maruyama、M.Shimomura 等人：“固体基质上分子聚集体的二维图案”固体薄膜（待发表）。

F.Nakamura, M.Shimomura, et al.: "Construction of two-dimensional DNA-Mimetics. Supramolecular Architecture with Highly Stacked Base-Pairs of Amphiphilic Adenine and Thymine at the Air-Water Interface." Thin Solid Films. (in press). (1998)

F.Nakamura、M.Shimomura 等人：“二维 DNA 模拟物的构建。在空气-水界面具有高度堆积的两亲性腺嘌呤和胸腺嘧啶碱基对的超分子结构。”