Trial study of versatile optical cell and equipment for high pressure spectroscopy

高压光谱通用光学池及设备的试验研究

• 批准号: 08556021
• 负责人: HAYASHI Rikimaru
• 金额: $ 6.91万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08556021/
• 关键词: high pressure; high pressure bioscience; high pressure spectroscopy; effect of high pressure; pressure inactivation; 圧力変性

To apply high pressure spectroscopy to researches in biochemistry and food science fields, this study was intended to construct 1)high pressure optical cells which were routinely used by being set to common spectroscopic apparatuses equipped in laboratories of universities, colleges. and company and government institutes, and 2)a high pressure spectrophotometer dedicated to measure absorption and fluorescence spectroscopy from far ultraviolet region to visible region.1.High pressure vessels with optical windows were constructed to fit an absorption spectrophotometer used in ultraviolet to visible region, a fluorescense spectrometer, and a circular dichroism polarimeter. Vessels were constructed with SUS-630 and their performance was as follows : maximum pressure, 500 MPa ; measurable wavelength, 200-700 nm, inside volume. 0.5-1.0 ml. Vessels for CD measurement was specially designed. Sample and pressure medium were separated by a free piston not to contact them. Several vessels were designed by using various window materials and sizes and their pressure tolerance and handling easiness were tested.2.High pressure spectrometer equipped with a high pressure vessel was constructed by applying an optical fiber system. This is used only in measuring absorption and flurescence of biological materials., The performance was as follows : maximum pressure. 500 MPa and measurable wavelength, 200-700nm.Using two method described above, high pressure effect on various proteins and enzymes were studied. But studies completely satisfying CD measurement had to be continuously in future.

为了将高压光谱应用于生物化学和食品科学领域的研究，本研究旨在构建1）常规使用的高压光学池，并将其安装在大学、学院实验室配备的普通光谱仪器上。以及公司和政府机构，以及2）专用于测量从远紫外区域到可见区域的吸收和荧光光谱的高压分光光度计。1.构造带有光学窗口的高压容器，以安装用于紫外到可见区域的吸收分光光度计、荧光光谱仪和圆二色性旋光计。容器采用SUS-630建造，其性能如下：最大压力500MPa；可测量波长，200-700 nm，内部体积。 0.5-1.0 毫升。用于 CD 测量的容器是专门设计的。样品和压力介质通过自由活塞分开，不接触它们。采用不同的窗口材料和尺寸设计了几种容器，并测试了它们的耐压性和操作简便性。2.采用光纤系统构建了配备高压容器的高压光谱仪。这仅用于测量生物材料的吸收和荧光。，性能如下：最大压力。压力500MPa，可测量波长200-700nm。利用上述两种方法，研究了高压对各种蛋白质和酶的影响。但完全满足CD测量的研究必须在未来不断进行。

项目成果

M.Dumoulin: "Textural properties of high pressure-induced gels of food protein obtained under different temperatures including zubzero" J.Food Sci.63(1)(in press). (1997)

M.Dumoulin：“在包括 zubzero 在内的不同温度下获得的高压诱导食品蛋白凝胶的质地特性”J.Food Sci.63(1)（印刷中）。

M.Kato: "Effects of high pressure and temperature on micelle formation of sodium dodecylsulfate" Lipids. 32(11). 179-185 (1997)

M.Kato：“高压和温度对十二烷基硫酸钠胶束形成的影响”脂质。

M.Kato et al.: "Effects of high pressure and temperature on micelle formation of sodium dodecylsulfate" Lipids. 32(11). 179-185 (1997)

M.Kato 等人：“高压和温度对十二烷基硫酸钠胶束形成的影响”脂质。

M.Dumoulin et.al.: "Textural properties of high pressure-induced gels of food protein obtained under different temperature including subzero" J.Food Sci.63(1), (in press). (1997)

M.Dumoulin 等人：“在不同温度（包括零度以下）下获得的高压诱导食品蛋白凝胶的质地特性”J.Food Sci.63(1)，（印刷中）。

M.Dumoulin: "Textural properties of high pressure-induced gels of food protein obtained under different temperatures including subzero" J.Food Sci.63(1)(in press). (1997)

M.Dumoulin：“在不同温度（包括零度以下）下获得的高压诱导食品蛋白凝胶的质地特性”J.Food Sci.63(1)（印刷中）。