Removal of sediment mud of lake and sea bottom and bioconversion to the biodegradable plastic using self-flocculated photosynthetic bacteria

利用自絮凝光合细菌去除湖泊和海底沉积泥并生物转化为可生物降解塑料

基本信息

批准号：
08650953
负责人：
SASAKI Ken
金额：
$ 1.54万
依托单位：
Hiroshima-Denki Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08650953/
关键词：
self-flocculation photosynthetic bacteria biodegradable plastic sediment mud PHB Rhodovulum sp.Rhodobacter

项目摘要

Focusing on the purification of sediment mud and bioconversion of mud to biodegradable plastic, firstly, chemical analyses of sediment mud were carried out. Sediment mud was consisted of 5% organic matters and 95% of sea sund and contained large amount of phosphorous compounds, After anaerobic digestion of mud suspension (200 g/l artificial sea wate) at 30 C for 7 days, ca. 2 g/l of acetic acid and Ca. 20 mg/l of phosphate ion was released into the broth. Using this anaerobic digestion liquor, a photosynthetic bacterial cultivation was carried out under anaerobic light condition (10 klux illumination) at 30 C.After 3 days culture, ca. 3 g/l of biomass was produced and almost phosphate was removed. Biomass produced contained 62% (dry basis) of polyhydroxybutyrate (PHB). Thus, the possibility of simultaneous purification of sediment mud and production of PHB was observed.Furthermore, high cell density culture of a newly isolated photosynthetic bacteria, Rhodovulum sp. which had the sel-flocculating ability was carried out using a new single tower bioreactor system to purify the anaerobic digestion liquor under aerobicculture condition eliminating light illumination. By this culture system, maximum biomass concentration attained 42.5 g/l after 750 hour culture with artifitial digestion liquor (acetic acid was a carbon source) and PHB content was ca. 30% even under aerobiccondition. In addition, a computer simulation was carried out using several biochemical engineering constants such as growth yield and some specific consumption rates. As the results, the estimations were in good agreement with the data obtained by experiments.Thus, a new bioreactor system become possible to develop by these investigations using this self-immobilizing photosynthetic bacteria. This indicates the possibility of the purification of sediment mud and simultaneous production of biodegradable plastic from sediment mud.

重点是将泥浆泥浆和泥浆纯化为可生物降解的塑料的纯化，首先，对沉积物泥的化学分析进行了分析。沉积物泥是由5％的有机物和95％的SEAD组成的，并在泥浆消化（200 g/l人工海Wate）在7天（CA）中含有大量磷酸化合物。 2 g/l的乙酸和大约。 20 mg/L的磷酸离子被释放到肉汤中。利用这种厌氧消化液，在30 C. 3天培养后，在30 C. C. 3天后进行光合细菌培养（10 klux照明）。产生了3 g/L的生物量，并去除几乎磷酸盐。产生的生物量含有62％（干基）的多羟基丁酸（PHB）。因此，观察到同时纯化沉积物泥浆和PHB的产生的可能性。FURTHERMORE，新分离的光合细菌的高细胞密度培养，Rhodovulum sp。使用新的单塔生物反应器系统实现了SEL-氯化能力，以净化厌氧消化液在有氧养殖条件下消除了光照明。通过这种培养系统，用伪装消化液（乙酸是一种碳源），在750小时培养后，最大生物量浓度达到42.5 g/L。即使在有氧核对条件下，也有30％。此外，使用几个生化工程常数（例如增长率和一些特定的消耗率）进行了计算机模拟。结果，估计与通过实验获得的数据非常吻合。因此，使用这种自动化的光合作用细菌可以通过这些研究进行新的生物反应器系统。这表明了沉积物泥浆纯化的可能性，并同时从沉积物泥浆中生产可生物降解的塑料。