Studies on the breeding of yellow-flowered evergreen azaleas through three way crossings.

黄花常绿杜鹃花三路杂交育种研究.

• 批准号: 08660035
• 负责人: MIYAJIMA Ikuo
• 金额: $ 1.41万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660035/
• 关键词: Evergreen azalea; Yellow-flowerwd azalea; Flower color; Carotenoid; Three way crossing; PCR-RFLP

Until now, a lot of crossings between Rhododendron japonicum and several evergreen azaleas were done for the obtainment of yellow-flowered evergreen azaleas. But almost all seedlings were albino and have never grown at anthesis.In 1996, we tried single and three way crossings between R.japonicum and evergreen azaleas and investigated the rate of capsule set, number of ovules, leaf color and the hybridity of the seedlings. The green seedlings were obtained in the three way crossings whereas those were not in the single crossings.In 1997, we investigated the relationship between leaf color and ptDNA by PCR-RFLP and discussed about the factor of the appearance of albino seedlings.The ptDNA of albino seedlings obtained from the crossings between Fl of evergreen azaleas and R.japonicum were originated in seed parents (maternal inheritance) whereas the ptDNA of the green seedlings were originated from pollen parents (paternal inheritance). In general, ptDNA of azaleas were usually inherited from seed parents.From these results, it was suggested that the appearance of albino seedlings related with the inheritance of ptDNA in the interspecific crossings in azaleas. In addition. the appearance of albino seedlings seem to be originated in the incompatibility between ptDNA and nuclear DNA.

迄今为止，为了获得黄花万年青杜鹃花，人们进行了大量的杜鹃花与几种万年青杜鹃花的杂交。1996年，我们用杜鹃花与万年青杜鹃花进行单交和三交试验，研究了它们的结实率、胚珠数、叶色和杂种优势。在三个交叉点获得了绿色幼苗，而在单交叉点没有。利用PCR-RFLP技术研究了万年青杜鹃花与杜鹃花杂交F1代获得的白化病苗的ptDNA与叶色的关系，并对影响白化病苗出现的因素进行了探讨（母本遗传），而绿色幼苗的ptDNA来源于花粉亲本（父本遗传）。杜鹃花ptDNA通常遗传自种子亲本，因此，杜鹃花种间杂交中白化病苗的出现与ptDNA的遗传有关。另外。白化病苗的出现可能起源于ptDNA与核DNA的不相容性。

项目成果

嬉野健次・宮島郁夫: "胚珠培養を利用したツツジ類の早期実生獲得について" 園芸学会雑誌. 65・別1. 362-363 (1996)

Kenji Ureshino 和 Ikuo Miyajima：“关于利用胚珠培养获得杜鹃花的早期幼苗”日本园艺学会杂志 65，第 1 部分。362-363 (1996)。

道下紋乃・嬉野健次・宮島郁夫: "ツツジ類の種間交雑育種における胚珠培養の利用" 園芸学会雑誌. 66・別2. 478-479 (1997)

Monno Michishita、Kenji Ureshino 和 Ikuo Miyajima：“胚珠培养在杜鹃花种间杂交育种中的应用”日本园艺学会杂志 66，第 2 部分。478-479 (1997)。

嬉野健次・宮島郁夫 他3名: "ツツジ類の種間雑種におけるアルビノの発現と葉緑体のDNAの遺伝性" 園芸学会雑誌. 66・別2. 480-481 (1997)

Kenji Ureshino、Ikuo Miyajima 和其他 3 人：“杜鹃花种间杂交中白化病和叶绿体 DNA 的遗传力”，日本园艺学会杂志 66，单独 2. 480-481 (1997)。

Michishita, A., K.Ureshino and I.Miyajima.: "Effectiveness of ovule culture in breeding of interspecific crossing of azaleas" J.Japan.Soc.Hort.Science. 66(Suppl.2). 478-479 (1997)

Michishita, A.、K.Ureshino 和 I.Miyajima.：“胚珠培养在杜鹃花种间杂交育种中的有效性”J.Japan.Soc.Hort.Science。

嬉野健次・宮島郁夫: "ツツジ類の多芽体形成を利用したin vitroでのコルヒチン処理" 園芸学会雑誌. 65・別2. 516-517 (1996)

Kenji Ureshino 和 Ikuo Miyajima：“利用杜鹃花多芽形成的体外秋水仙碱处理”，《园艺学会杂志》65，第 2 部分。516-517 (1996)。