Molecular basis of establishment of basic compatibility in Pyricularia fungi.

梨锈病真菌基本相容性建立的分子基础。

• 批准号: 08660057
• 负责人: NOZU Mikio
• 金额: $ 1.6万
• 依托单位: SHIMANE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660057/
• 关键词: rice blast; basic compatibility; host-selective toxin; ultrastructure; イネいもち病; 突然変異イネ; 毒素; レース; 微細構造; ミトコンドリア

Magnaporthe grisea produces a host-selective toxin the spore germination fluid, which allow a non-pathogen, Alternaria alternata, to infect host plant tissues. The role of a toxin on establishment of basic compatibility between fungus and plant was investigated by ultrastructural observation and morphometric measurement. The first toxin-induced changes in ultrastructure were th losses of mitochondrial matrix and the disruption of mitochondrial cristae 1hr after toxin exposure. However, frequency of modified mitochondria did not increase with time after toxin treatment. Other organelles were normal even in the late stage of the toxin treatment. These phenomena were observed in both Sekiguchi-asahi and Asahi leavesregardless of light condition. When the effects of the toxin (s) on host plants (rice and barley) and non-host plant (finger millet) were examined, the first toxin-induced changes in rice and barley plants were the loss of the mitochondrial matrix and the disruption of mitochondrial cristae. These modifications in mitochonria were observed 1 hr after toxin exposure. However, the frequency of modified mitochondria did not increase with time after toxin treatment. Other organelles were normal even in a late stage of the toxin treatment. Such phenomena were observed in three sites (epidermal, mesophyll and phloem cells) of host plants, barley and rice. However, the effects of toxin (s) were not observed in leaves of non-host plant, finger millet. Further, mitochondrial modifications were observed in both epidermal cells of compatible and incompatible cultivars 12 hr after inoculation of M.grisea spores. These results indicated that mitochondrial modification induced by the toxin (s) was an essential event for M.grisea to establish basic compatibility with host plants at species level.

稻瘟病菌产生一种寄主选择性毒素--孢子萌发液，它允许非病原菌互隔交链孢霉侵染寄主植物组织。通过超微结构观察和形态计量学测量，研究了毒素在真菌与植物之间建立基本亲和性中的作用。毒素诱导的超微结构改变首先是毒素暴露后1小时线粒体基质丢失和线粒体脊断裂。然而，毒素处理后线粒体修饰频率并不随时间的延长而增加。其他细胞器即使在毒素处理的后期也是正常的。无论光照条件如何，这些现象在关口朝日和朝日的叶片中都能观察到。当研究S毒素对寄主植物(水稻和大麦)和非寄主植物(指谷)的影响时，首先引起水稻和大麦植株的变化是线粒体基质丢失和线粒体脊断裂。在毒素暴露后1小时观察到线粒体膜的这些改变。然而，毒素处理后线粒体修饰的频率并没有随着时间的延长而增加。其他细胞器即使在毒素处理的后期也是正常的。在寄主植物大麦和水稻的三个部位(表皮细胞、叶肉细胞和韧皮部细胞)中观察到了这种现象。而在非寄主植物指谷的叶片中，未观察到S毒素的作用。此外，在接种稻瘟病菌孢子12小时后，亲和和不亲和品种的表皮细胞中都观察到线粒体的修饰。这些结果表明，S毒素诱导的线粒体修饰是稻瘟病菌与寄主植物在物种水平上建立基本亲和性的重要事件。

项目成果

Uehara, T.et al.: "Primary effects of Magnaporthe grisea toxin (s) on mitochondria of rice leaf cells." Ann.Phytopathol.So.Jpn.63 (1). 29-35 (1997)

Uehara, T.et al.：“稻瘟病菌毒素对稻叶细胞线粒体的主要影响。”

Arase, S.et al.: "Molecular Genetics of Host-Specific Toxins in Plant Disease" K.Kohmoto and O.C.Yoder (印刷中), (1997)

Arase, S. 等人：“植物病害中宿主特异性毒素的分子遗传学”K.Kohmoto 和 O.C.Yoder（出版中），(1997)

Uehara, T.et al.: "Ultrastructural effects of Magnaporthe grisea toxin (s) on mitochondria of host and non-host plants." Ann.Phytopathol.Soc.Jpn.63 (2). 69-77 (1997)

Uehara, T.et al.：“稻瘟病菌毒素对宿主和非宿主植物线粒体的超微结构影响”。

Uehara, T.et al.: "Primary effects of Magnaporthe grisea toxin(s) on mitochondria of rice leaf cells." Ann.Phytopathol.Soc.Jpn.63. 29-35 (1997)

Uehara, T.等人：“稻瘟病菌毒素对稻叶细胞线粒体的主要影响。”

Arase,S.et al.: "Light-dependent induction of Sekiguchi lesion by Mgnaporthe grisea infections in rice cv Sekiguchi-asahi" J.Phytopath.144(印刷中). (1996)

Arase, S. 等人：“水稻品种 Sekiguchi-asahi 中稻瘟病菌感染对 Sekiguchi 病变的光依赖性诱导”J.Phytopath.144（出版中）。