RT-PCR Expression analysis of ACC oxidase gene and ACC synthase gene in melon leaf in field condition.

研究了大田条件下ME-ACS1和CM-ACO1基因在不同基因表达诱导剂诱导下的表达模式。(1)与甜瓜GAPDH基因相比，甜瓜肌动蛋白基因是有用的内参基因。(2)RT-PCR结果表明，在田间条件下，这两个基因的表达都受到伤害、IAA和乙烯的诱导。(3)这两个基因都受到热激胁迫(38ﾟC)的诱导，在10min内表达达到最大，在1h达到最大值，直到6h。(4)热激1h后，甜瓜平台恢复到25ﾟC，(5)在叶片衰老过程中，ME-ACS1mRNA水平不变，而CM-ACO1mRNA水平逐渐降低。

We investigated the expression pattem of the ME-ACS 1 and CM-ACO 1 genes in the response to various inducers of gene expression in field condition.(1) The melon actin gene is useful internal standard, compare with the melon GAPDH gene..(2) Results of RT-PCR showed that the expression of both genes were induced by wounding, IAA,Ethylene in field condition.(3) Both genes were induced by heat shock stress (38ﾟC) with in 10 min, expression reaching a maximum at 1 hr.and decreasing until 6 h.(4) After heat shock for 1h.melon plats were returned to 25ﾟC,ME-ACS 1 mRNA level gradually decreased, but level of CM-ACO 1 mRNA rewired high for 6 h.(5) ME-ACS 1 mRNA level was constant during leaf senescence, but CM-ACO 1 mRNA level was gradually decreased.