RT-PCR Expression analysis of ACC oxidase gene and ACC synthase gene in melon leaf in field condition.

RT-PCR 田间条件下甜瓜叶片 ACC 氧化酶基因和 ACC 合酶基因的表达分析

• 批准号: 08660041
• 负责人: FURUKAWA Hajime
• 金额: $ 0.9万
• 依托单位: OSAKA PREFECTURE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660041/
• 关键词: Ethylene; Melon; ACC oxidase gene; ACC synthase gene; Gene expression; RT-PCR; ACC酸化酵素; ACC合成酵素

We investigated the expression pattem of the ME-ACS 1 and CM-ACO 1 genes in the response to various inducers of gene expression in field condition.(1) The melon actin gene is useful internal standard, compare with the melon GAPDH gene..(2) Results of RT-PCR showed that the expression of both genes were induced by wounding, IAA,Ethylene in field condition.(3) Both genes were induced by heat shock stress (38ﾟC) with in 10 min, expression reaching a maximum at 1 hr.and decreasing until 6 h.(4) After heat shock for 1h.melon plats were returned to 25ﾟC,ME-ACS 1 mRNA level gradually decreased, but level of CM-ACO 1 mRNA rewired high for 6 h.(5) ME-ACS 1 mRNA level was constant during leaf senescence, but CM-ACO 1 mRNA level was gradually decreased.

我们研究了ME-ACS 1和CM-ACO 1基因在田间条件下对不同基因表达诱导剂的表达模式。(1)与甜瓜GAPDH基因相比，甜瓜肌动蛋白基因是有用的内标。(2) RT-PCR结果表明，田间条件下，损伤、IAA、乙烯均能诱导这两个基因的表达。(3)两种基因均在热休克（38℃）胁迫下在10 min内表达，在1 h表达量达到最大值。(4)热休克1h后。(5)在叶片衰老过程中，ME-ACS 1 mRNA的表达水平保持不变，而CM-ACO 1 mRNA的表达水平逐渐下降。

项目成果

古川一: "PCRによるメロンのACC合成酵素遺伝子の増幅" 園芸学会雑誌. 65別2. 288-289 (1996)

Hajime Furukawa：“通过PCR扩增甜瓜ACC合酶基因”日本园艺学会杂志65 Betsu 2. 288-289 (1996)。

Hajime FURUKAWA: "RT-PCR expression analysis of ACC synthasegene and ACC oxidase gene in melon leaf" Plat Cell Physiol.3S S4ppl.117-117 (1997)

Hajime FURUKAWA：“甜瓜叶中 ACC 合成酶基因和 ACC 氧化酶基因的 RT-PCR 表达分析”Plat Cell Physiol.3S S4ppl.117-117 (1997)

Hajime FURUKAWA: "Amplification of ACC synthase gene of melon." J.Japan.Soc.Hort.Sci.suppl.2. 144 (1996)

Hajime FURUKAWA：“甜瓜 ACC 合酶基因的扩增。”

Hajime FURUKAWA: "Tne melon polyubiguitin gene is inducel by HEat Shock" J.Japan.Soc.Hort.Sci.67S4ppl.1. 144-144 (1998)

Hajime FURUKAWA：“热休克诱导甜瓜多聚泛素基因”J.Japan.Soc.Hort.Sci.67S4ppl.1。

Hajime FURUKAWA: "RT-PCR expression analysis of ACC synthase gene and ACC oxidase gene in melon leaf" Plant Cell Physiol.38.suppl.117-117 (1997)

Hajime FURUKAWA：“甜瓜叶中 ACC 合酶基因和 ACC 氧化酶基因的 RT-PCR 表达分析”Plant Cell Physiol.38.suppl.117-117 (1997)