The Inducing Effect of Thiamin on Fruit Body Formation of Lentinula edodes

在蛋白胨-葡萄糖液体培养基中，研究了硫胺素对香菇营养菌丝生长和子实体形成的影响。硫胺素是子实体形成所必需的，其最低需要量约为10 μ g/L。添加0-1.5mg/L的硫胺素对菌丝生长影响不大。三次对菌丝生长的抑制并不明显。即使在使用不含维生素的酪蛋白氨基酸或谷氨酸作为氮源而不是蛋白胨的情况下，由于将菌丝体转移到新鲜培养基中，也不会发生菌丝体生长的硫胺素缺乏。在9周培养的前3周期间，几乎所有培养基中含有的硫胺素都被吸收并积累在菌丝体中。这些结果表明，香菇菌丝体的营养生长只需要微量的硫胺素。因为香菇需要大约10 μ g/L硫胺素用于出菇，所以添加到基础培养基中的所有硫胺素必须用于子实体形成。

The effects of thiamin on vegetative mycelial growth and fruit body formation of Lentinula edodes were investigated in basal peptone-glucose liquid media in relation to the uptake of thiamin. Thiamin is essential for fruit body formation, and the minimum requirements for thiamin are estimated to be approximately 10 mug/L.The vegetative mycelial growth was little influenced by the addition of thiamin in the range of 0-1.5mg/L.While the mycelia was successively transferred to fresh peptone-glucose-agar media. three times, the repression of mycelial growth was not significant. Even in cases using vitamin-free casamino acid or glutamic acid as a nitrogen source instead of peptone, a thiamin deficiency for mycelial growth did not occur as a result of transferring the mycelia to fresh media. Almost of all the thiamin contained in the media was taken and accumulated in the mycelia during the first 3 weeks of a 9-week incubation. These results suggested that only trace amounts of thiamin are required for the vegetative mycelial growth of Lentinula edodes. Because Lentinula edodes requires approximately 10 mug/L thiamin for fruiting, all the thiamin added to the basal media must be used for fruit body formation.