The Inducing Effect of Thiamin on Fruit Body Formation of Lentinula edodes

研究了硫胺素对香菇营养菌丝生长和子实体形成的影响及其与硫胺素吸收的关系。硫胺素是子实体形成所必需的，硫胺素的最低需要量约为10 mg/L。在0-1.5 mg/L范围内添加硫胺素对菌丝营养生长影响不大。三次对菌丝生长的抑制作用不显著。即使在使用不含维生素的酪氨酸或谷氨酸作为氮源而不是蛋白胨的情况下，将菌丝体转移到新鲜的培养基中也不会导致菌丝生长的硫胺素缺乏。在9周培养的前3周，几乎所有培养液中所含的硫胺素都被摄取并在菌丝体中积累。这些结果表明，香菇的营养菌丝生长只需要微量的硫胺素。由于香菇结实大约需要10杯/L硫胺素，所以添加到基本培养基中的硫胺素必须全部用于子实体的形成。

The effects of thiamin on vegetative mycelial growth and fruit body formation of Lentinula edodes were investigated in basal peptone-glucose liquid media in relation to the uptake of thiamin. Thiamin is essential for fruit body formation, and the minimum requirements for thiamin are estimated to be approximately 10 mug/L.The vegetative mycelial growth was little influenced by the addition of thiamin in the range of 0-1.5mg/L.While the mycelia was successively transferred to fresh peptone-glucose-agar media. three times, the repression of mycelial growth was not significant. Even in cases using vitamin-free casamino acid or glutamic acid as a nitrogen source instead of peptone, a thiamin deficiency for mycelial growth did not occur as a result of transferring the mycelia to fresh media. Almost of all the thiamin contained in the media was taken and accumulated in the mycelia during the first 3 weeks of a 9-week incubation. These results suggested that only trace amounts of thiamin are required for the vegetative mycelial growth of Lentinula edodes. Because Lentinula edodes requires approximately 10 mug/L thiamin for fruiting, all the thiamin added to the basal media must be used for fruit body formation.