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Studies on trophtype of neritic

浅海营养型的研究

基本信息

批准号：
08660215
负责人：
ODATE Tsuneo
金额：
$ 0.83万
依托单位：
National Institute of Polar Research (1997)Hokkaido University (1996)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660215/
关键词：
Dinoflagellates trophic type Funka Bay 渦鞭毛藻

项目摘要

Oceanographic observations and plankton samplings were conducted at 14 stations in Funka Bay from July, 1996 to Octover, 1997. Seawater samples (2-5 litters) were collected from 0-50 m depth with ca. 10 m interval using Niskin samplers. The seawater was filtered using 200 ummeshed screen, and plankters in the filtrate were collected with 20 um meshed screen. The plankton samples were fixed with buffered formalin (2% v/v) and was kept inrefrigerator. These samples were prepared for microscopic observation using an epifluorescence microscope. At the same time, seawater samples for determinations of nutrient concentrations using Auto Analyzer and chlorophyll a concentration by fluorometry. Extraction of chlorophyll a was conducted using N,N-dimethylformamide. Furthermore, maximum depth that a vertically towed Norpac net reached and underwater movement of the net were investigated using a micro depth data logger. Some studies which were partly supported by this Grant-in-Aid for Scientific Research (C) (2) (#08660215) have been already published (Odate et al., 1998 : Imai et al., in press : Iinuma et al., in press). Microscopic observations are conducted now. The results will be published in near future.

1996年7月至1997年10月，在丰卡湾的14个站位进行了海洋观测和浮游生物采样。用尼斯金取样器从0-50m深度采集海水样品(2-5滴)，间隔约10m。海水用200um筛网过滤，滤液中浮游生物用20um筛网收集。浮游生物样品用缓冲福尔马林(2%v/v)固定并保存在冰箱中。这些样品被准备用荧光显微镜进行显微镜观察。同时，海水样品用自动分析仪测定营养物质浓度，用荧光法测定叶绿素a浓度。以N，N-二甲基甲酰胺为萃取剂提取叶绿素a。此外，还利用微深度数据记录仪研究了NORPAC垂直拖网达到的最大深度和网的水下运动。科学研究补助金(C)(2)(#08660215)部分支持的一些研究已经发表(Odate等人，1998年：Imai等人，出版社：Iinuma等人，出版社)。现在正在进行显微镜观察。研究结果将在不久的将来公布。