Taxonomic status of Fasciola in Japan : Comparison of mitochondrial NDI and COI sequences and ribosomal ITS2 sequence with Fasciola hepatica and Fasciola gigantica

日本片形吸虫的分类地位：肝片形吸虫和巨大片形吸虫的线粒体 NDI 和 COI 序列以及核糖体 ITS2 序列的比较

• 批准号: 08660357
• 负责人: ITAGAKI Tadashi
• 金额: $ 1.28万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660357/
• 关键词: Japanese Fasciola; F.hepatica; F.gigantica; Mitochondrial DNA; Ribosomal DNA; Nucleotide sequence; COI; NDI; ITS-2

The purpose of the study is to compare nucleotide sequences of mitochondrial NDI and COI and ribosomal ITS2 among Fasciola species, and to detect DNA polymorphism within triploid form of Fasciola in Japan. The analysis of restriction fragment length polymorphism shows that triploid Japanese Fasciola species is composed of at least two populations possessing respective type of mitochondrial DNA.It is probable that maternal origin of triploid Japanese species of the two populations is not F.hepatica, but rather F.gigantica. The one population of triploid Japanese species and diploid Korean species, both reproducing off spring parthenogenetically, would originate from the same ancestral F.gigantica. The ND1 and COI sequences of Japanese triploid forms of Fasciola were nearly identical to those found in F.gigantica, but were different from those of F.hepatica. Thus, the Japanese triploid forms of Fasciola are thought to be categorized to F.gigantica. The ITS-2 sequences of the Japanese triploid worms were divided into two distinct types, one of which was almost identical to that of F.hepatica and the other to F.gigantica from Indonesia.

本研究的目的是比较日本片形吸虫不同种的线粒体NDI和COI以及核糖体ITS 2的核苷酸序列，并检测日本片形吸虫三倍体形式的DNA多态性。限制性片段长度多态性分析表明，三倍体日本片形吸虫至少由两个具有各自线粒体DNA类型的群体组成，这两个群体的三倍体日本片形吸虫的母系起源可能不是肝片形吸虫，而是南极片形吸虫。一个人口的三倍体日本种和二倍体韩国种，都繁殖后代的春天孤雌生殖，将来自同一祖先F. arthritica。ND 1和COI序列的日本三倍体形式的片形吸虫是几乎相同的发现在F. acutica，但不同的肝片形吸虫。因此，日本的片形吸虫的三倍体形式被认为被归类为F. acetica。日本三倍体蠕虫的ITS-2序列可分为两种不同的类型，一种与肝片吸虫的ITS-2序列几乎相同，另一种与印度尼西亚的亚洲片吸虫的ITS-2序列几乎相同。

项目成果

Itagaki T.et al.: "Taxonomic status of the Japanese triploid forms of Fasciola:Comparison of mitochondrial ND1 and COI sequences with F.hepatica and F.gigantica" J.Parasitol.(In press).

Itagaki T.et al.：“日本片形吸虫三倍体形式的分类地位：线粒体 ND1 和 COI 序列与 F.hepatica 和 F.gigantica 的比较”J.Parasitol.（正在出版）。

ITagaki T et al.: "Taxonomic status of the Japanesetriploid forms of Fasciola:Comparision of mitochondrial NDI and COI sequences with F.hepatica and F.gigantica" J.Parasitol. (In press).

Itagaki T 等人：“日本片形吸虫三倍体形式的分类地位：线粒体 NDI 和 COI 序列与 F.hepatica 和 F.gigantica 的比较”J.Parasitol。

Itagaki T.: "Comparison of mitochondrial DNA sequences among Fasciola species." The Japanese Sciety for Systematic Parasitology. 15. 19-22 (1997)

Itagaki T.：“片形吸虫属物种线粒体 DNA 序列的比较。”

Itagaki T.& Tsutsumi K.: "Triploid form of Fasciola in Japan:Genetic relationships between Fasciola hepatica and Fasciola gigantica determined by ITS-2 sequence of nuclear rDNA" Int.J.Parasitol.In press.

板垣T.

ITagaki T.&Tsutsumi K.: "Triploid form of Fasciola in Japan:Genetic relationships between Fasciola hepatica and Fasciola gigantica determined by ITS-2 sequence of nuclear rDNA." Int.J.Parasitol.(In press).

板垣 T.