Protective immune responses in enteromucosa tissues of cats infected with Toxoplasma gondii

感染弓形虫的猫肠粘膜组织的保护性免疫反应

• 批准号: 08660376
• 负责人: OMATA Yoshitaka
• 金额: $ 1.66万
• 依托单位: Obihiro University of Agriculture & Veterinary Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660376/
• 关键词: Toxoplasma gondii; cats; enteroepithelial tissues; Interleukin 12; IgA; protective immunity; stage of Gametogony; lgA

1) Identification of interleukin 12 in various tissue organs of cats infected with Toxoplasma gondii ; Interleukin 12 -35kD and -40 kD subunits of cats were identified by RT-PCR.In the spleen and mesenteric lymphnodes of cats reinfected with T.gondii, IL-12 40kD subunit was expressed transiently post reinfection.2) Detection of secretory IgA and its protective roles in enteromucosa tissues of cats ; Anti-T.gondii antibody IgA was detected in feces and intestinal washings ofcats infected with T.gondii. Supplement of the washings in the culture medium suppressed the parasite penetration activity of feline embryonic fibroblasts.3) Stage-spcific antigens associated with gametogenesis of Toxoplasma gondii ; Enteroepithelial stage parasites were isolated by Percoll density gradient centrifugation. Enteroepithelial stage parasite specific antigens were detected by antisera of mice and cats immunized with the parasites.

1）弓形虫感染猫各组织器官中白细胞介素12的鉴定； RT-PCR鉴定猫白细胞介素12 -35kD和-40 kD亚基。再感染猫的脾脏和肠系膜淋巴结中，IL-12 40kD亚基在再感染后短暂表达。2）分泌型IgA及其对猫肠粘膜组织的保护作用的检测；在感染弓形虫的猫的粪便和肠洗液中检测到抗弓形虫抗体IgA。培养基中洗涤液的补充抑制了猫胚胎成纤维细胞的寄生虫穿透活性。3）与弓形虫配子发生相关的阶段特异性抗原；通过 Percoll 密度梯度离心分离肠上皮期寄生虫。通过用寄生虫免疫的小鼠和猫的抗血清检测肠上皮期寄生虫特异性抗原。

项目成果

Yoshitaka OMATA, et al.: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradient centrifugation" Parasitology Research. 83. 574-577 (1997)

Yoshitaka OMATA 等人：“通过 Percoll 密度梯度离心从猫的肠粘膜中分离弓形虫的球虫肠上皮阶段”寄生虫学研究。

Yoshitaka OMATA, et al.: "Positive evidence that anti-Toxoplasma gondii IgA antibody exists in the intestinal tract of infected cats and exerts positive activitv against the infection" Veterinary Parasitology. 73. 1-11 (1997)

Yoshitaka OMATA 等人：“积极证据表明，受感染猫的肠道中存在抗弓形虫 IgA 抗体，并对感染发挥积极活性”《兽医寄生虫学》。

Yoshitaka OMATA et al.: "Positive evidence that anti-Toxoplasma gondii IgA antibody exists in the intestinal tract of infected cats and exerts positive activity against the infection." Veterinary Parasitology. 73. 1-11 (1997)

Yoshitaka OMATA 等人：“积极证据表明，受感染猫的肠道中存在抗弓形虫 IgA 抗体，并对感染发挥积极活性。”

Yoshitaka OMATA et al.: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradient centrifugation." Parasitology Research. 83. 574-577 (1997)

Yoshitaka OMATA 等人：“通过 Percoll 密度梯度离心从猫的肠粘膜中分离弓形虫的球虫肠上皮阶段。”

Yoshitaka OMATA: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradient centrifugation" Parasitology Research. (印刷中).

Yoshitaka OMATA：“通过 Percoll 密度梯度离心从猫的肠粘膜中分离出弓形虫的球虫肠上皮阶段”寄生虫学研究（正在出版）。