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The role of the receptor tyrosine kinase, Cek8, in chick embryos, especially on the somite dofferentiation.

受体酪氨酸激酶 Cek8 在鸡胚中的作用，特别是对体节分化的作用。

基本信息

批准号：
08670008
负责人：
HIRANO Shigeki
金额：
$ 1.41万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670008/
关键词：
chick embry Cek8 receptor tyrosine kinase somite neural tube notochord celldeath induction 体節分化椎板分化筋分化

项目摘要

The spatio-temporal pattern of expression of the Eph receptor tyrosine kinase, Cek8, was oberved in the formation of somites in chich embryos from H-H stage 6 to 23 using immunohistochemical techniques in combination with several microsurgical manipulations to isolate the segmental plate from axial structures. Observation in unmanipulated embryos revealed that the spatio-temporal patterns of Cek8 expression appeared to have an intimate relationship with tissue rebuilding rebuilding, the maturation of differentiated cells, and the spatial organization of tissue. Consequently, it is likely that Cek8 plays an integral role in the developmental events leading to the formation of many, but not all, tissue and organ systems. Cek8 expression initally appeared prior to the differentiation of sclerotomes and disappeared after the segregation of sclerotomes and dermomyotomes. On the other hand, on alteration in the pattern of Cek8 expression was observed in somites which were surgically separated from neighboring tissue. These observations suggest that Cek8 expression has an intimate relationship with sclerotome differentiation but apparently is not regulated by the surrounding tissue.

利用免疫组织化学技术结合显微外科操作分离节段板和轴向结构，观察了从H-H期6至23期胚胎体形成过程中Eph受体酪氨酸激酶Cek8的时空表达模式。在未处理的胚胎中观察发现，Cek8的时空表达模式似乎与组织重建重建、分化细胞成熟和组织空间组织密切相关。因此，Cek8很可能在导致许多（但不是全部）组织和器官系统形成的发育事件中起着不可或缺的作用。Cek8的表达最初出现在硬核细胞分化之前，在硬核细胞和真皮细胞分离后消失。另一方面，在手术分离的体细胞中观察到Cek8表达模式的改变。这些观察结果表明，Cek8的表达与硬核组分化密切相关，但显然不受周围组织的调节。