The development studies on the peripheral nervous systems and muscles used LacZ gene recombinant adenovirus

神经和肌肉之间有着密切的一一对应的关系。为了生存，需要相互依存。它还没有得到解决仍然如此密切的关系。此外，标签是必要的神经研究。本研究以LacZ基因重组腺病毒为新的细胞标记材料，以鸡胚为材料，首先考察了LacZ基因重组腺病毒对周围神经和肌肉的标记情况，阐明了周围神经和肌肉的胚胎学关系。观察周围神经在肢芽内的形成过程，寻找神经纤维延伸部位的结构。此外，受体型扭转激酶及其配体的表达模式进行了观察，以阐明在肢芽有关的神经分支的分子环境。此外，在肌祖细胞在肢芽中迁移和个体肌块形成过程中，神经和肌肉之间存在着何种关系。LacZ基因重组腺病毒是神经系统发育研究中较为方便的标记物。感觉神经和运动神经利用相同的环境延伸它们的纤维。提示受体型扭转激酶及其配体在肢芽中呈背腹互补表达模式，分别诱导周围神经纤维束的背侧支和腹侧支。在这段时间里，似乎血管成为了一个障碍，提高了方向决定的准确性。很明显，神经和肌肉的发育没有直接的关系，它们独立地推进了它们的发育过程。

Nerve and muscle have the close relationship between the one-to-one. In order to survive, the mutuality is required. It has not been solved still such close relationships. In addition, labels are necessary to the neuroresearches. In this study, LacZ gene recombination adenovirus was used as a material of new cell marking and clarified the embryological relation between the peripheral nerves and the muscles.The chick embryos were used as a material, and at first, how the nerve was labeled in the virus, was examined. Then, the formation process of peripheral nerve in limb bud was observed, and the structure of the positions which extended the nerve fibers was searched. Further, the expression patterns of receptor type torsion kinase and its ligand was observed in order to elucidate the molecular environment in the limb buds concerning the branching of nerves. In addition, what kind of relation was observed between the nerves and the muscles during the migration of muscle progenitor cells in the limb bud and the formation of the individual muscle mass.LacZ gene recombination adenovirus is the comparatively convenient mark for the developmental research on the nervous system. The sensory and motor nerves extended their fibers using the same environment. It was suggested that the receptor type torsion kinase and its ligand, which showed the dorsal-ventral complementary expression pattern in the limb bud induced the dorsal and ventral branches of the peripheral nerve fiber bundles, respectively. In this time, it seems that the blood vessel became a barrier and raised the accuracy of the direction decision. It became clear that the development of the nerves and muscles did not have the direct relationship, and they advanced their developmental processes independently.