The development studies on the peripheral nervous systems and muscles used LacZ gene recombinant adenovirus

LacZ基因重组腺病毒对周围神经系统和肌肉的开发研究

• 批准号: 11670004
• 负责人: HIRANO Shigeki
• 金额: $ 2.3万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670004/
• 关键词: Adenovirus; gene recombination; LacZ; peripheral nerve; muscle; development; アデノウイルス

Nerve and muscle have the close relationship between the one-to-one. In order to survive, the mutuality is required. It has not been solved still such close relationships. In addition, labels are necessary to the neuroresearches. In this study, LacZ gene recombination adenovirus was used as a material of new cell marking and clarified the embryological relation between the peripheral nerves and the muscles.The chick embryos were used as a material, and at first, how the nerve was labeled in the virus, was examined. Then, the formation process of peripheral nerve in limb bud was observed, and the structure of the positions which extended the nerve fibers was searched. Further, the expression patterns of receptor type torsion kinase and its ligand was observed in order to elucidate the molecular environment in the limb buds concerning the branching of nerves. In addition, what kind of relation was observed between the nerves and the muscles during the migration of muscle progenitor cells in the limb bud and the formation of the individual muscle mass.LacZ gene recombination adenovirus is the comparatively convenient mark for the developmental research on the nervous system. The sensory and motor nerves extended their fibers using the same environment. It was suggested that the receptor type torsion kinase and its ligand, which showed the dorsal-ventral complementary expression pattern in the limb bud induced the dorsal and ventral branches of the peripheral nerve fiber bundles, respectively. In this time, it seems that the blood vessel became a barrier and raised the accuracy of the direction decision. It became clear that the development of the nerves and muscles did not have the direct relationship, and they advanced their developmental processes independently.

神经与肌肉之间有着一一对应的密切关系。为了生存，相辅相成是必需的。它还没有解决，还有如此密切的关系。此外，标签对于神经研究也是必要的。本研究利用LacZ基因重组腺病毒作为新的细胞标记材料，阐明了周围神经和肌肉之间的胚胎学关系。以鸡胚胎为材料，首先考察了神经在病毒中的标记情况。然后，观察周围神经在肢芽中的形成过程，寻找神经纤维伸展部位的结构。此外，还观察了受体型扭转激酶及其配体的表达模式，以阐明肢芽中与神经分支有关的分子环境。此外，在肢芽中肌祖细胞的迁移和个体肌块的形成过程中，神经和肌肉之间存在着什么样的关系。LacZ基因重组腺病毒是神经系统发育研究较为方便的标志。感觉神经和运动神经在相同的环境中伸展纤维。结果提示，在肢芽中呈背腹互补表达模式的受体型扭转蛋白及其配体分别诱导了周围神经纤维束的背侧和腹侧分支。在这段时间里，血管似乎成了障碍，提高了方向决策的准确性。很明显，神经和肌肉的发育并没有直接的关系，它们独立地推进着自己的发育过程。