Morphological and biochemical study of TSH-recetor expression

TSH受体表达的形态学和生化研究

• 批准号: 08670196
• 负责人: MIZUKAMI Yuji
• 金额: $ 1.47万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670196/
• 关键词: TSH-receptor; thyroid; immunohistochemistry; in situ hybridization; northern blot hybridization; nothern blot hybridization; thyroglobulin; peroxidase

In the present study, we investigated TSH-receptor expression in hyman thyroids using immunohistochemistry, in situ hybridization and northern blot. The relationship between the TSH-receptor expression and the thyroid function were examined.A.Materials and MethoedsThe surgically resected thyroid tissues of various thyroid diseases were used in this study. The thyroid specimens were immediately frozed at -80C and stored. The TSH-receptor cDNA vector and the anti-human mouse monoclomnal antibody against TSH-receptor protein were used. These were kindly supplied by Dr.E.Milgrom (paris South University, France).B.ResultsThe obtained results were as follows :1. The immunohistochemical, in situ hybridization and northern blot study showed the same results in the expression of TSH-receptor in human thyroids of each case. examined.2. In Graves' thyroids, the elevated TSH-receotor expression was observed in both of protein level and mRNA level. This finding supported the theory that the up-regulation mechanism of TSH-receptor is one of the major causes of Graves' hyperthyroidism.3. In the thyroid adenomas and the carcinomas which are functionally inactive, the elevated TSH-receptor expression was observed in the protein level and mRNA level.4. In the undifferentiated thyroid carcinomas, The TSH-receptor expression was not detected in the protein level and mRNA level.

在本研究中，我们使用免疫组织化学，原位杂交和北印迹研究了海曼甲状腺中的TSH受体表达。研究了TSH受体表达与甲状腺功能之间的关系。材料和甲基甲基化的手术切除的各种甲状腺疾病的甲状腺组织在本研究中使用。立即将甲状腺标本以-80C冻结并储存。使用了针对TSH受体蛋白的TSH受体cDNA载体和抗人小鼠单闭抗体。这些由e.milgrom博士（法国巴黎南大学）提供。免疫组织化学的原位杂交和北印迹研究在每种情况的人类甲状腺中表达TSH受体的表达相同。检查2。在Graves的甲状腺中，在蛋白质水平和mRNA水平上都观察到TSH-RECEOTOR表达升高。这一发现支持这样的理论：TSH受体的上调机制是坟墓甲状腺功能亢进症的主要原因之一。3。在功能无效的甲状腺腺瘤和癌中，在蛋白质水平和mRNA水平上观察到TSH受体表达升高。4。在未分化的甲状腺癌中，未在蛋白质水平和mRNA水平中检测到TSH受体表达。