Morphological and biochemical study of TSH-recetor expression

在本研究中，我们研究了TSH受体的表达在海曼甲状腺免疫组织化学，原位杂交和北方印迹。A.材料和方法本研究采用各种甲状腺疾病手术切除的甲状腺组织。甲状腺标本立即在-80 ℃下冷冻保存。使用TSH受体cDNA载体和抗人鼠TSH受体蛋白的单克隆抗体。这些由E.Milgrom博士（巴黎南方大学，法国）提供。B.结果获得的结果如下：1.免疫组化、原位杂交和北方印迹分析结果表明，两例甲状腺组织中TSH受体的表达情况相同。检查。在Graves甲状腺中，TSH受体在蛋白质水平和mRNA水平均表达增高。这一发现支持了TSH受体上调机制是Graves甲亢发病的主要原因之一的理论.在甲状腺腺瘤和甲状腺癌中，TSH受体的表达在蛋白水平和mRNA水平上均升高.在未分化型甲状腺癌中，TSH受体在蛋白水平和mRNA水平均未检测到表达。

In the present study, we investigated TSH-receptor expression in hyman thyroids using immunohistochemistry, in situ hybridization and northern blot. The relationship between the TSH-receptor expression and the thyroid function were examined.A.Materials and MethoedsThe surgically resected thyroid tissues of various thyroid diseases were used in this study. The thyroid specimens were immediately frozed at -80C and stored. The TSH-receptor cDNA vector and the anti-human mouse monoclomnal antibody against TSH-receptor protein were used. These were kindly supplied by Dr.E.Milgrom (paris South University, France).B.ResultsThe obtained results were as follows :1. The immunohistochemical, in situ hybridization and northern blot study showed the same results in the expression of TSH-receptor in human thyroids of each case. examined.2. In Graves' thyroids, the elevated TSH-receotor expression was observed in both of protein level and mRNA level. This finding supported the theory that the up-regulation mechanism of TSH-receptor is one of the major causes of Graves' hyperthyroidism.3. In the thyroid adenomas and the carcinomas which are functionally inactive, the elevated TSH-receptor expression was observed in the protein level and mRNA level.4. In the undifferentiated thyroid carcinomas, The TSH-receptor expression was not detected in the protein level and mRNA level.