mRNA expression of TSH-receptor, calcitonin and thyroglobulin in medullary carcinoma of the thyroid ; A study of histogenesis of mixed medullary-follicular carcinoma of the thyroid

甲状腺髓样癌中 TSH 受体、降钙素和甲状腺球蛋白 mRNA 的表达；

基本信息

批准号：
10670156
负责人：
MIZUKAMI Yuji
金额：
$ 1.73万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We studied to clarify the histogenesis of mixed medullary-follicular carcinoma of the thyroid using methods of clinicopathology, immunohistochemistry, in situ hybridization and northern blot. The results are as follows :1). Clinicopathologically, mixed medullary-follicular carcinomas are more frequently occurs compared with usual medullary carcinoma : in male patients (6 : 1 vs 1 : 1), in younger patients (36yrs vs 44yrs) and with better prognosis (100% vs 78% in 5-year survival).2). We failed to detection of calcitonin and thyroglobulin mRNA using northern blot, probably due to error of extraction of mRNA from thyroid tissues.3). Using in situ hybridization and immunohistochemistry, mRNA and protein of calcitonin or thyroglobulin are detected in mixed medullary-follicular carcinoma.4). We initially expected that mRNA or protein of calcitonin and thyroglobulin are concomitantly express in tumor cells of mixed medullary-follicular carcinoma. However, in the present study, such a concomitant expression of calcitonin and thyroglobulin in the same tumor cell was not observed.5). The concomitant expression of calcitonin and thyroglobulin was not observed in usual medullary carcinomas and other types of thyroid cancers.We will continue intensively the research for clarification of histogenesis of medullary-follicular carcinoma of thyroid.

我们研究了使用临床病理学，免疫组织化学，原位杂交和北印迹的方法，以阐明甲状腺混合髓样癌的组织发生。结果如下：1）。在临床病理学上，混合髓质癌的混合癌与通常的髓瘤相比：男性患者（6：1 vs 1：1），在年轻患者中（36岁vs 44岁），预后更好（5年生存中的100％vs 78％）。我们没有使用北印迹检测降钙素和甲状腺球蛋白mRNA，这可能是由于从甲状腺组织中提取mRNA的错误。3）。使用原位杂交和免疫组织化学，在混合髓质 - 粉状癌中检测到降钙素或甲状腺球蛋白的mRNA和蛋白质。4）。我们最初期望降钙素和甲状腺球蛋白的mRNA或蛋白质在混合髓质粉状癌的肿瘤细胞中同时表达。但是，在本研究中，未观察到降钙素和甲状腺球蛋白在同一肿瘤细胞中的这种伴随表达。5）。在通常的髓质癌和其他类型的甲状腺癌中未观察到降钙素和甲状腺球蛋白的伴随表达。我们将继续进行深入的研究，以阐明甲状腺甲状腺髓质癌的组织发生。