mRNA expression of TSH-receptor, calcitonin and thyroglobulin in medullary carcinoma of the thyroid ; A study of histogenesis of mixed medullary-follicular carcinoma of the thyroid

本研究采用临床病理、免疫组织化学、原位杂交和Northern印迹等方法，对甲状腺髓-滤泡型混合癌的组织发生机制进行了研究。研究结果如下：1)。在临床病理上，混合型髓样-滤泡型癌比普通髓样癌更常见：男性(6：1比1：1)，年轻患者(36年比44年)，预后更好(5年生存率100%比78%)。Northern杂交未能检测到降钙素和甲状腺球蛋白的mRNA，这可能是由于从甲状腺组织中提取的mRNA错误所致。应用原位杂交和免疫组织化学方法检测髓滤泡混合性癌组织中降钙素或甲状腺球蛋白的表达。我们最初预计在髓滤泡混合性癌的肿瘤细胞中同时表达降钙素和甲状腺球蛋白的mRNA或蛋白。然而，在目前的研究中，没有观察到降钙素和甲状腺球蛋白在同一肿瘤细胞中同时表达。降钙素和甲状腺球蛋白在常见的髓样癌和其他类型的甲状腺癌中未见伴发表达，我们将继续深入研究甲状腺髓样滤泡癌的组织起源。

We studied to clarify the histogenesis of mixed medullary-follicular carcinoma of the thyroid using methods of clinicopathology, immunohistochemistry, in situ hybridization and northern blot. The results are as follows :1). Clinicopathologically, mixed medullary-follicular carcinomas are more frequently occurs compared with usual medullary carcinoma : in male patients (6 : 1 vs 1 : 1), in younger patients (36yrs vs 44yrs) and with better prognosis (100% vs 78% in 5-year survival).2). We failed to detection of calcitonin and thyroglobulin mRNA using northern blot, probably due to error of extraction of mRNA from thyroid tissues.3). Using in situ hybridization and immunohistochemistry, mRNA and protein of calcitonin or thyroglobulin are detected in mixed medullary-follicular carcinoma.4). We initially expected that mRNA or protein of calcitonin and thyroglobulin are concomitantly express in tumor cells of mixed medullary-follicular carcinoma. However, in the present study, such a concomitant expression of calcitonin and thyroglobulin in the same tumor cell was not observed.5). The concomitant expression of calcitonin and thyroglobulin was not observed in usual medullary carcinomas and other types of thyroid cancers.We will continue intensively the research for clarification of histogenesis of medullary-follicular carcinoma of thyroid.