mRNA expression of TSH-receptor, calcitonin and thyroglobulin in medullary carcinoma of the thyroid ; A study of histogenesis of mixed medullary-follicular carcinoma of the thyroid

本文应用临床病理、免疫组化、原位杂交和北方印迹等方法对甲状腺髓样滤泡混合癌的组织发生进行了研究。结果如下：1）.在临床病理上，混合型髓样滤泡癌与普通髓样癌相比更常见：男性患者（6：1 vs 1：1），年轻患者（36岁vs 44岁）和预后更好（5年生存率100% vs 78%）。北方杂交未能检测到降钙素和甲状腺球蛋白mRNA，可能是由于甲状腺组织mRNA提取错误所致。应用原位杂交和免疫组化技术检测髓滤泡混合癌中降钙素和甲状腺球蛋白的mRNA和蛋白表达。我们最初认为降钙素和甲状腺球蛋白的mRNA或蛋白在髓滤泡混合癌的肿瘤细胞中同时表达。然而，在本研究中，降钙素和甲状腺球蛋白在同一肿瘤细胞中的这种伴随表达并未被排除。降钙素和甲状腺球蛋白在普通髓样癌和其他类型的甲状腺癌中未同时表达，我们将继续深入研究，以阐明甲状腺髓样滤泡癌的组织发生。

We studied to clarify the histogenesis of mixed medullary-follicular carcinoma of the thyroid using methods of clinicopathology, immunohistochemistry, in situ hybridization and northern blot. The results are as follows :1). Clinicopathologically, mixed medullary-follicular carcinomas are more frequently occurs compared with usual medullary carcinoma : in male patients (6 : 1 vs 1 : 1), in younger patients (36yrs vs 44yrs) and with better prognosis (100% vs 78% in 5-year survival).2). We failed to detection of calcitonin and thyroglobulin mRNA using northern blot, probably due to error of extraction of mRNA from thyroid tissues.3). Using in situ hybridization and immunohistochemistry, mRNA and protein of calcitonin or thyroglobulin are detected in mixed medullary-follicular carcinoma.4). We initially expected that mRNA or protein of calcitonin and thyroglobulin are concomitantly express in tumor cells of mixed medullary-follicular carcinoma. However, in the present study, such a concomitant expression of calcitonin and thyroglobulin in the same tumor cell was not observed.5). The concomitant expression of calcitonin and thyroglobulin was not observed in usual medullary carcinomas and other types of thyroid cancers.We will continue intensively the research for clarification of histogenesis of medullary-follicular carcinoma of thyroid.