mRNA expression of TSH-receptor, calcitonin and thyroglobulin in medullary carcinoma of the thyroid ; A study of histogenesis of mixed medullary-follicular carcinoma of the thyroid

应用临床病理学、免疫组织化学、原位杂交和northern blot等方法，探讨甲状腺髓样-滤泡混合癌的组织发生机制。结果表明：1)。临床病理上，与普通髓样癌相比，混合性髓样-滤泡癌更常发生：男性患者（6:1 vs 1:1），年轻患者（36岁vs 44岁），预后较好（5年生存率100% vs 78%）。我们无法通过northern blot检测降钙素和甲状腺球蛋白mRNA，可能是由于从甲状腺组织中提取mRNA的错误。应用原位杂交和免疫组化技术，检测骨髓-滤泡混合癌中降钙素或甲状腺球蛋白的mRNA和蛋白表达。我们最初预计降钙素和甲状腺球蛋白的mRNA或蛋白在混合性髓样-滤泡癌的肿瘤细胞中同时表达。然而，在本研究中，没有观察到降钙素和甲状腺球蛋白在同一肿瘤细胞中同时表达。在普通髓样癌和其他类型的甲状腺癌中未观察到降钙素和甲状腺球蛋白的同时表达。我们将继续深入研究甲状腺髓样滤泡癌的组织发生机制。

We studied to clarify the histogenesis of mixed medullary-follicular carcinoma of the thyroid using methods of clinicopathology, immunohistochemistry, in situ hybridization and northern blot. The results are as follows :1). Clinicopathologically, mixed medullary-follicular carcinomas are more frequently occurs compared with usual medullary carcinoma : in male patients (6 : 1 vs 1 : 1), in younger patients (36yrs vs 44yrs) and with better prognosis (100% vs 78% in 5-year survival).2). We failed to detection of calcitonin and thyroglobulin mRNA using northern blot, probably due to error of extraction of mRNA from thyroid tissues.3). Using in situ hybridization and immunohistochemistry, mRNA and protein of calcitonin or thyroglobulin are detected in mixed medullary-follicular carcinoma.4). We initially expected that mRNA or protein of calcitonin and thyroglobulin are concomitantly express in tumor cells of mixed medullary-follicular carcinoma. However, in the present study, such a concomitant expression of calcitonin and thyroglobulin in the same tumor cell was not observed.5). The concomitant expression of calcitonin and thyroglobulin was not observed in usual medullary carcinomas and other types of thyroid cancers.We will continue intensively the research for clarification of histogenesis of medullary-follicular carcinoma of thyroid.