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Purification of a novel T cell-derived eosinophil chemotactic factor and its significance in allergic diseases

新型T细胞源性嗜酸性粒细胞趋化因子的纯化及其在过敏性疾病中的意义

基本信息

批准号：
08670253
负责人：
HIRASHIMA Mitsuomi
金额：
$ 1.47万
依托单位：
Kagawa Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

Our established T cell line cells (STO-2) produce five eosinophil chemotactic factors (ECF) with different isoelectric point (pI-5, pI-6, pI-7, pI-8, and pI-9). They exhibit different biological functions in eosinophils. We have also found that most of them are different factors from the identified chemotactic factors such as eotaxin and RANTES.We assessed chemotactic response of eosinophils to the above STO-2-derived ECF in patients with diseases characterized by eosinophilia as described below.1.Eosinophil response differs according to the presence or absence of dermal symptom in Kimura's disease.2.In atopic dermatitis, the response differs according to the presence of complications and disease severity.3.The response in asthmatic patients differs according to the disease severity, especially in the requirement of steroid therapy.4.In eosinophilic pneumonia, we can make differential diagnosis between acute and chronic eosinophilic pneumonia by the difference of the eosinophil response.Now, we have succeeded cDNA cloning of one of STO-2-derived ECF,ECF-pI7 which is also produced from T cells during the specific antigenic stimulation. We have also found that the factor (ecalectin) is a variant of galectin-9 that is one of galactoside-binding lectins. We are now going to clarify the biological functions and the significance of ecalectin in human allergic diseases.

我们建立的T细胞系细胞（STO-2）产生五种具有不同等电点（pI-5，pI-6，pI-7，pI-8和pI-9）的嗜酸性粒细胞趋化因子（ECF）。它们在嗜酸性粒细胞中表现出不同的生物学功能。我们还发现它们中的大多数是与鉴定的趋化因子如嗜酸性粒细胞趋化因子和RANTES不同的因子。我们评估了嗜酸性粒细胞对上述STO-2衍生的ECF的趋化反应，其特征在于嗜酸性粒细胞增多症，如下所述。1.嗜酸性粒细胞的反应根据木村病中皮肤症状的存在或不存在而不同。2.在特应性皮炎中，反应根据并发症和疾病严重程度的存在而不同。3.哮喘患者的反应根据疾病严重程度而不同，特别是在需要类固醇治疗时。4.在嗜酸性粒细胞性肺炎中，我们可以通过嗜酸细胞反应的差异来鉴别急性和慢性嗜酸细胞性肺炎。现在，我们已经成功地克隆了STO-2衍生的ECF之一ECF-pI 7的cDNA，该ECF-pI 7也是在特异性抗原刺激期间由T细胞产生的。我们还发现该因子（ecalectin）是半乳糖苷结合凝集素之一的半乳糖凝集素-9的变体。我们现在将阐明ecalectin的生物学功能及其在人类过敏性疾病中的意义。