HSP 70 of Cryptococcus neoformans in Cryptococcosis.

隐球菌病中新型隐球菌的 HSP 70。

• 批准号: 08670667
• 负责人: KOHNO Shigeru
• 金额: $ 1.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670667/
• 关键词: Anti-C.neoformans; HSP 70 antibody; Cryptococcus neoformans; Pulmonary cryptococcosis; ATPase; Capsular antigen; HSP; cryptococcus neo-Reemans; cryptococcosis; HSP 70; ストレス蛋白(HSP); ワクチン; Th-1,Th-2反応; ストレス蛋白質(HSP)

Cryptococcus neoformans causes infection in individuals with defective T-cell function, such as AIDS, as well as without underlying disease. It has been suggested that humoral as well as cellular immunity might play an important role in the immune response to C.neoformans infection. We have recently shown, using immunoblotting, that the 70-kDa heat shock protein (HSP) family of C.neoformans was the major target molecule of the humoral response in murine pulmonary cryptococcosis. In this study, we also used immunoblotting to define the antibody responses in the sera of 24 patients with pulmonary cryptococcosis - 21 proven and 3 suspected diagnoses. Anti-C.neoforinans HSP 70 antibody was detected in 16 of 24 (66.7%) patients with pulmonary cryptococcosis. Fourteen of 17 (82.3%) patients with high antigen titers (*1 : 8) and 2 of 7 (28.6%) patients with low titers (*1 : 4), had detectable levels of anti-HSP 70 antibody. Sera from patients positive for anti-HSP 70 antibody showed high titers in the Eiken latex agglutination test for the detection of serum cryptococcal antigen. Our results indicate that the 70-kDa heat shock protein family from C.neoforinans appears to be a major target molecule of the humoral response, not only in murine pulmonary cryptococcosis, but also in human patients with pulmonary cryptococcosis.

新型隐球菌引起T细胞功能缺陷的个体感染，如艾滋病，以及没有基础疾病。体液免疫和细胞免疫在新生隐球菌感染的免疫应答中起重要作用。我们最近用免疫印迹法证明，新生隐球菌的70 kDa热休克蛋白（HSP）家族是小鼠肺隐球菌病体液应答的主要靶分子。在这项研究中，我们还使用免疫印迹法来确定24例肺隐球菌病患者血清中的抗体反应- 21例确诊和3例疑似诊断。24例肺隐球菌病患者中16例（66.7%）检出抗新生隐球菌HSP 70抗体。17例高滴度（*1：8）和7例低滴度（*1：4）患者中分别有14例（82.3%）和2例（28.6%）可检测到抗HSP 70抗体。抗HSP 70抗体阳性的患者血清在Eiken乳胶凝集试验检测血清隐球菌抗原时显示高滴度。我们的研究结果表明，70 kDa的热休克蛋白家族从C.neoforinans似乎是一个主要的靶分子的体液反应，不仅在小鼠肺隐球菌病，但也在人类患者肺隐球菌病。

项目成果

Kakeya H.et al.: "Heat shock protein 70 (HSP70) as a major target of the antibody response in patients with pulmorary cryptococcosis" Clin Exp Emmunol. (印刷中). (1999)

Kakeya H. 等人：“热休克蛋白 70 (HSP70) 作为肺隐球菌病患者抗体反应的主要靶点”Clin Exp Emmunol（出版中）。

Kakeya H.et al.: "A 77 kilodalton Protein of Cryptococcus neoformans a meiuucv of heat shock protein 70 familly, iso major antigen detected in the sera of nice with poluoway cryptococcus" Infect Immun. 65. 1653-1658 (1997)

Kakeya H.等人：“新型隐球菌的 77 kilodalton 蛋白是热休克蛋白 70 家族的 meiuucv，在 poluoway 隐球菌的血清中检测到异主要抗原”感染免疫。

Kakeya H, et al.: "A 77-kilodalton protein of Cryptococcus neoformans, a member of the heat shock protein 70 family, is a major antigen detected in the sera of mice with pulmonary cryptococcosis." Infect Immun. 65. 1653-1658 (1997)

Kakeya H 等人：“新型隐球菌的 77 千道尔顿蛋白是热休克蛋白 70 家族的成员，是在患有肺隐球菌病的小鼠血清中检测到的主要抗原。”

木村 哲: "肺クリプトコックス症におけるHSP抗原の関与" エイズと日和見感染症に関する臨床研究. 70-74 (1996)

Satoshi Kimura：“HSP抗原参与肺隐球菌病”艾滋病和机会性感染的临床研究70-74（1996）。

Kakeya H, et al.: "Heat shock protein 70 (HSP 70) as a major target of the antibody response in patients with pulmonary cryptococcosis." Clin Exp Immunol. (in press). (1999)

Kakeya H 等人：“热休克蛋白 70 (HSP 70) 是肺隐球菌病患者抗体反应的主要目标。”