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Neuropathological study of astrocytic fibrillary tangles

星形细胞纤维缠结的神经病理学研究

基本信息

批准号：
08670734
负责人：
MIZUTANI Tomohiko
金额：
$ 1.22万
依托单位：
Nihon University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670734/
关键词：
astrocyte fibrillary change astrocytic fibrillary tangle astrocytic fibrillary tangle

项目摘要

Neurofibrillary tangles have been known for years to occur in the neurons of the hippocampus and cerebral cortex of patients with Alzheimer type dementia. Similar fibrillary changes also occur in the astrocytes of a certain degenerative diseases, particularly progressive supranuclear palsy and corticobasal degeneration. Such astrocytic fibrillary tangles (AFTs), first reported in 1992, have been stained with anti-tau antibodies. However, we found novel, tau-negative AFTs in 2 patients with familial Parkinson disease. We investigated the brains of various diseases to elucidate the specificity and heterogeneity of AFTs. We stained sections of the brain by methenamine silver and Gallyas-Braak methods, and the sections which contained AFT were further immunostained with anti-tau antibodies. We found tau-positive AFTs in 1 patient with corticobasal degeneration, and tau-negative AFTsin 1 patient with schizophrenia, in addition to 2 patients with the familial Parkinson disease. We also studied the methenamine silver-stained section of the tau-negative AFTs with electron microscope, but could not identify the exact constituents of the AFTs. In assition, we found numerous neurofibrillary tangles in the neurons of the second to third layrs of the cerebral cortex in the 2 patients with familial Parkinson disease. The tangles consisted of 15-nm straight tubules similar to those of progressive supranuclear palsy, but were tau-negative. Such tau-negative neurofibrillary tangles have not been reported previously. Patients with the familiar Parkinson disease has been published in Acta Neuropathologica (95 : 15-27, 1998), including the 2 patients who showed the new types of tau-negative AFTs and neurofibrillary tangles.

多年来，人们已经知道阿尔茨海默型痴呆患者的海马神经元和大脑皮层中会出现神经原纤维缠结。在某些退行性疾病，特别是进行性核上性麻痹和皮质基底性变性中，星形胶质细胞也会发生类似的纤维变化。这种星形细胞纤维缠结(AFT)于1992年首次报道，已经被抗tau抗体染色。然而，我们在2名家族性帕金森病患者中发现了新的tau阴性AFT。我们研究了各种疾病的大脑，以阐明AFTS的特异性和异质性。用亚甲胺银和Galyas-Braak法对脑切片进行染色，对含有AFT的切片进一步用抗tau抗体进行免疫组织化学染色。除2例家族性帕金森病外，1例皮质基底性变性患者为tau阳性，1例精神分裂症患者为tau阴性。我们还用电子显微镜研究了tau阴性AFTs的亚甲胺银染切片，但无法确定AFTs的确切成分。我们在2例家族性帕金森病患者的大脑皮质第二至第三层神经元中发现了大量的神经原纤维缠结。这些缠结由15 nm的直管组成，类似于进行性核上性麻痹，但tau阴性。这种tau阴性的神经原纤维缠结以前没有报道过。常见的帕金森病患者已发表在《神经病理学学报》(95：15-27,1998)上，其中包括2例表现为tau阴性的AFT和神经原纤维缠结的患者。