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Histopathologic study of novel inclusions in the ependymal cells of the caudal medulla and spinal cord

尾髓质和脊髓室管膜细胞中新型包涵体的组织病理学研究

基本信息

批准号：
12670622
负责人：
MIZUTANI Tomohiko
金额：
$ 2.18万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670622/
关键词：
ependymal cell ubiquitin inclusion body tanycyte

项目摘要

Ubiquitin-positive inclusion (UbIs) have not been well studied in the ependymal cells yet. Since we encountered such UbIs in the central canals of the spinal cords and caudal medullae while investigating UbIs in neurodegenerative diseases, we investigated the UbIs by performing various staining methods and examining the ependymas of the central canals of the spinal cords and caudal medullas of 46 patients with 25 kinds of neurological diseases and 7 non-neurological controls. We also examined the ependymas of the lateral and 3rd ventricles, cerebral aqueduct, and 4th ventricles. The UbIs were located in the cytoplasm of the ependymal cells, round to oval in shape, measuring 2 to 8 μm in diameter. UbIs were also occasionally found in ependymal residual nests. The UbIs were mildly reactive to periodic acid Schiff staining with and without digestion, but not stained by other methods including hematoxylin and eosin staining. The UbIs were unreactive to various antibodies other than those to ubiquitin. UbIs were found in 36 out of 46 patients with various neurological diseases, and in 7 out of 7 non-neurological diseases, thus not specific to any particular diseases. UbIs were most often observed in the ependymal cells of the central canals of the spinal cords and caudal medullae, and only a few UbIs in the lateral, 3rd and 4th ventricles. The significance of the ependymal UbIs is unclear at present. The central canals and remnant ependymal cells do not appear to active ependymal function, so that the frequent presence of UbIs at such inactive sites may indicates that UbIs represent some degenerative changes which occurred to ependymal cells.

室管膜细胞中的泛素阳性包涵体 (UbIs) 尚未得到充分研究。由于我们在研究神经退行性疾病中的 UbIs 时在脊髓中央管和尾髓中遇到了此类 UbIs，因此我们通过执行各种染色方法并检查 46 例 25 种神经系统疾病患者和 7 个非神经系统对照患者的脊髓中央管和尾髓管室管膜来研究 UbIs。我们还检查了侧脑室和第三脑室、脑导水管和第四脑室的室管膜。 UbIs位于室管膜细胞的细胞质中，形状为圆形至椭圆形，直径为2至8μm。室管膜残留巢中也偶尔发现 UbIs。 UbIs 对经过或不经过消化的高碘酸希夫染色有轻微反应，但不被包括苏木精和伊红染色在内的其他方法染色。 UbIs 对除泛素以外的各种抗体均不发生反应。在 46 名患有各种神经系统疾病的患者中，有 36 人发现了 UbIs，在 7 种非神经系统疾病中，有 7 人发现了 UbIs，因此不针对任何特定疾病。 UbIs最常在脊髓中央管和尾髓的室管膜细胞中观察到，仅在侧脑室、第三脑室和第四脑室中观察到少量UbIs。目前室管膜 UbIs 的意义尚不清楚。中央管和残余室管膜细胞似乎没有活跃的室管膜功能，因此在这些不活跃的部位频繁出现UbIs可能表明UbIs代表了室管膜细胞发生的一些退行性变化。