Involvement of transcription factors for STATs in hypertensive vascular hypertrophy

STATs转录因子参与高血压血管肥大

• 批准号: 08670818
• 负责人: NEGORO Nobuo
• 金额: $ 1.54万
• 依托单位: OSAKA CITY UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670818/
• 关键词: HYPERTENSION; ARTERIOSCLERUSIS; MOLECULAR MECHANISM; HYPERTROPHY; TRANSCRIPTION FACTOR; 血管平滑筋; 肥大; STAT

1.Involvements of transcription factors in hypertrophy of cultured vascular smooth muscle cells (VSMC)(1)DNA decoys are short ds-oligonucleotides corresponding to the Sp1 and ISRE,GAS,SIE,which are consensus sequences of STAT1 and STAT2. Random decoys were used as negative control. The decoys in multi-lamellar cationic liposome were intracellular-transferred to angiotensin II (AII)-treated rat VSMCs.(2)The levels of 3H-leucine uptake in AII-treated VSMCs were inhibited by the decoy transfers for Sp1 and ISRE,GAS,SIE (69%, 58%, 66%, 41%)in association with decreases of PDGF A-chain mRNA level and 3H-thymidine uptake.2.Involvements of STAT1 and STAT2 in hypertensive arteriosclerosis in spontaneously hypertensive rats (SHR)(1)Transcription factors for GAS and SIE were increased in thoracic aortic medial layrs in SHR in association with increases of PDGF-A chain mRNA level.(2)These increases were inhibited by the administration of type 1 of AII receptor antagonist.3.Effects of cellular transfer of DNA decovs for STAT 1 and STAT 2 to femoral arteries of SHR(1)The decoy transfer for ISRE,SIE and GAS inhibited the vascular hypertrophy index (85%, 88%, 81%) without lowering blood pressure.(2)But the random DNA decoy did not inhibit it.These results indicates that transcription factors for STATs are closely involved in the development of hypertensive vascular hypertrophy in vivo.

1.转录因子参与培养的血管平滑肌细胞（VSMC）肥大的研究（1）DNA诱饵是与STAT 1和STAT 2的共有序列Sp1和ISRE、GAS、SIE相对应的短的双链寡核苷酸。随机诱饵用作阴性对照。将多层阳离子脂质体中的诱饵转移到血管紧张素II（AII）处理的大鼠VSMCs中。（2）Sp1和ISRE、GAS、SIE的诱骗转移均抑制AII处理的VSMCs摄取~ 3 H-亮氨酸的水平（69%，58%，66%，STAT 1和STAT 2在自发性高血压大鼠（SHR）高血压动脉硬化中的作用（1）SHR胸主动脉中层GAS和SIE转录因子表达增加，并与PDGF-A链mRNA表达增加相关。（2）STAT 1和STAT 2的DNA诱骗转移对SHR股动脉的影响（1）ISRE、SIE和GAS的诱骗转移在不降低血压的情况下，抑制血管肥厚指数（85%，88%，81%）。（2）随机DNA诱饵对STATs的表达无明显抑制作用，提示STATs转录因子参与了高血压血管肥厚的发生发展。

项目成果

Negoro, N.et al.: "Transcription factors for PDGF A-chain gene closely mediated hypertensive vascular hypertrophy in vitro and in vivo." Circulation. 94. 1739 (1996)

Negoro, N.等人：“PDGF A 链基因的转录因子在体外和体内密切介导高血压血管肥大。”

Negoro, N.et al.: "Transcription factors for PDGF-A chain gene closely mediated hypertensive vascular hypertrophy in vitro and in vivo." J.Hypertension. 14. S32-S32 (1996)

Negoro, N.等人：“PDGF-A 链基因的转录因子在体外和体内密切介导高血压血管肥大。”

Negoro, N.et al.: "Transcription factoros for PDGF A-chain gene closely mediated hypertensive vascular hypertrophy in vitro and in vivo." J Hypertension. 14. S72 (1996)

Negoro, N.等人：“PDGF A 链基因的转录因子在体外和体内密切介导高血压血管肥大。”

Nobuo Negoro,et al.: "Transcription factors for PDGF-A chain gene closely mediated hypertensive vascular hypertrophy in vitro and in vivo." Circulation. 94. I-739-I-740 (1996)

Nobuo Negoro 等人：“PDGF-A 链基因的转录因子在体外和体内密切介导高血压血管肥大。”

Nobuo Negoro, et al.: "Transcription factors for PDGF-A chain gene closely mediated hypertensive vascular hypertrophy in vitro and in vivo." Circulation. 94. I-739-I-740 (1996)

Nobuo Negoro 等人：“PDGF-A 链基因的转录因子在体外和体内密切介导高血压血管肥大。”