Effects of sex steroids on growth of rats

性类固醇对大鼠生长的影响

• 批准号: 08670851
• 负责人: OGAWA Eishin
• 金额: $ 1.15万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670851/
• 关键词: GHD rat; growth; IGF-1; estradiol; testosterone; puberty; GH binding; ラット

*EFFECTS OF LONG-TERM GONADOTROPIN-RELEASING HORMONE ANALOGUE TREATMENT ON SOMATIC GROWTE AND CIRCULATING INSULIN-LIKE GROWTH FACTOR-1 LEVELS IN THE GROWTH HOIRMONE DEFICIENT RAT*[AIM] Previous studies suggest that gonadectomy lead to slight reduction of body weight gain with increased or unchanged insulin-like growth factor (IGF) -1 levels in male rats, and increased both weight gain and IGF-1 levels in female rats. We speculate that perinatal gonadal steroids and elevated gonadatropins may affect growth and the growth hormone (GH) /IGF-1 axis. This study was designed to evaluate the effects of hypogonadotropic gonadal suppression since neonatal period on somatic growth and serum IGF-1 levels in rats.[MATERIALS and METHODS] Spontaneous dwarf rat was used as a model of complete GH deficiency (GHD), and Sprague-Dawlry (SD) rat was used as control.Gonadotropin-releasing hormone analogue (GnRHa : Leuprolide acetate depot, 2mg/kg) was administered subcutaneously every 3 weeks. Treatment wa … More s started within 48 hours after birth and terminated at 15 weeks of age. Body weight, serum testosterone (T)or estradiol (E2), and IGF-1 were evaluated, and effects were compared with saline treated rats.[RESULTS] Serum T or E2 were significantly reduced by the GnRHa treatment. Body weight at 15 was not different between the groups in both lines of male rats (saline vs GnRHa ; 551(]SY.+-。[)43 vs 561(]SY.+-。[)27g in SD rats, 88(]SY.+-。[))15 vs 91(]SY.+-。[)12g in GHD rats). While in famale, markedly greater body weight in the GnRHa group was observed in SD rats (285(]SY.+-。[)7 vs 351(]SY.+-。[))31g, p<0.01), but not in GHD rats (79(]SY.+-。[)15 vs 79ア16g). Serum IGF-1 was not different between the groups in both rats of both male (957 ア173 vs 976(]SY.+-。[)97ng/ml in SD rats, 10.3(]SY.+-。[)1.0 vs 11.5(]SY.+-。[)1.3ng/ml in GHD rats) and female (460(]SY.+-。[)167 vs 534(]SY.+-。[)74ng/ml in SD rats, 11.6(]SY.+-。[)3.1 vs 11.0(]SY.+-。[))1.8ng/ml in GHD rats).[SUMMARY] These data indicate that GnRHa induced gonadal suppression did not affect somatic growth or IGF-1 in male rats, while suppressed gonadotropin-estrogen axis resulted in increased body weight without changes in circulating IGF-1 levels in female rats, and this effect appears to be mediated through GH. Less

*长期促性腺激素释放激素类似物治疗对生长激素缺乏大鼠躯体生长和循环胰岛素样生长因子-1水平的影响*[目的]先前的研究表明，性腺切除术导致雄性大鼠体重增加轻微减少，但胰岛素样生长因子(IGF) -1水平升高或不变，雌性大鼠体重增加和IGF-1水平均增加。我们推测围产期性腺激素和促性腺激素升高可能影响生长和生长激素(GH) /IGF-1轴。本研究旨在探讨新生儿期开始的促性腺激素抑制对大鼠体生长和血清IGF-1水平的影响。【材料与方法】以自发性侏儒大鼠作为完全性生长激素缺乏症（GHD）模型，以SD大鼠为对照。促性腺激素释放激素类似物（GnRHa：醋酸Leuprolide depot， 2mg/kg）每3周皮下注射一次。治疗在出生后48小时内开始，并在15周龄时终止。评估体重、血清睾酮(T)或雌二醇（E2）和IGF-1，并与生理盐水处理的大鼠进行比较。[结果]GnRHa治疗显著降低血清T或E2。两种雄性大鼠15岁时的体重在两组之间没有差异（生理盐水vs GnRHa； 551 [SY.+-]）。[)43 vs . 561(][) SD大鼠27g， 88(]SY.+-。[1] [1] [1] [1] [1][) GHD大鼠12g)。而在雌性SD大鼠中，GnRHa组的体重明显增加（285 [SY.+-]）。[)7 vs 351 [][))31g, p<0.01)，但在GHD大鼠中无统计学意义(79(]SY.+-。[)15 vs 79 （16g）。两雄性大鼠血清IGF-1组间差异无统计学意义（957±173对976）。()在SD大鼠97 ng / ml, 10.3 (SY。+ -。)(SY。+ - 1.0 vs 11.5。(GHD) 1.3 ng / ml老鼠)和女性(460 (SY。+ -。[)167 vs . 534 []()在SD大鼠74 ng / ml, 11.6 (SY。+ -。)(SY。+ - 3.1 vs 11.0。(GHD)) 1.8 ng / ml老鼠)。【摘要】这些数据表明，GnRHa诱导的性腺抑制不影响雄性大鼠的体生长和IGF-1，而抑制促性腺激素-雌激素轴导致雌性大鼠体重增加，但不改变循环IGF-1水平，这种影响可能是通过GH介导的。少