IDENTIFICATION OF NEW GLOMERULAR PROGRESSION FACTORS USING MONOCLONAL ANTIBODY

使用单克隆抗体鉴定新的肾小球进展因子

基本信息

  • 批准号:
    08670894
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

In order to identify a new cell surface antigen as a potential marker of renal glomerular cell injury, we produced a monoclonal antibody (Mab), 1F3, by immunizing mice with cultured human glomerular cells. Immunohistochemical studies demonstraated that 1F3 recognizing molecule (1F3 molecule) was strongly expressed on the cell surface of glomerular podocytes and very weakly on parietal epithelial cells in the normal kidney. Immunoprecipitation revealed that 1F3 recognized a 125 KDa protein under reducing conditions. Sequenced N-terminal amino acid analysis following 1F3 immunoaffinity purification indicated that 1F3 molecule resembled integrin alpha3 subunit. Biopsy specimens from patients with a variety of glomerular and tubulointerstitial diseases showed that 1F3 antigen was almost negative in cellular crescents but was strongly expressed in fibro-cellular crescents. 1F3 antigen became positive in atrophic tubules that were seen in diseased kidneys. Severity of tubular atrophy correlated well with the extent of tubular expression of 1F3 antigen. Urine examination using ELISA showed that increased excretion of 1F3 molecule was observed in the patients with glomerulonephritis as compared with healthy donor. These results indicate that Mab, 1F3 marks cell biological changes of renal epithelial cells under disease conditions and may be a useful marker for progressive kidney diseases.
为了鉴定一种新的细胞表面抗原作为肾小球细胞损伤的潜在标志物,我们用培养的人肾小球细胞免疫小鼠,制备了单克隆抗体(Mab)1F 3。免疫组化结果显示,1F 3识别分子(1F 3 molecule,1F 3 molecule)在正常肾脏肾小球足细胞表面呈强阳性表达,在壁上皮细胞表面呈弱阳性表达。免疫沉淀显示,1F 3识别一个125 KDa的蛋白在还原条件下。1F 3免疫亲和纯化后的N-末端氨基酸序列分析表明,1F 3分子类似于整合素α 3亚基。各种肾小球和肾小管间质疾病患者的活检标本显示,1F 3抗原在细胞性新月体中几乎为阴性,但在纤维细胞性新月体中强烈表达。1F 3抗原在病变肾萎缩小管中呈阳性。肾小管萎缩的严重程度与肾小管1F 3抗原表达的程度相关。用ELISA法检测肾小球肾炎患者尿中1F 3分子的排泄量,结果显示肾小球肾炎患者尿中1F 3分子的排泄量明显高于正常人。这些结果表明,Mab,1F 3标志着疾病条件下肾上皮细胞的细胞生物学变化,并可能是一个有用的标志物进行性肾脏疾病。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
森本 雄次: "ヒト糸球体上皮細胞に対すモノクロナール抗体1F3:腎上皮細胞障害の新しいマーカー" 日本小児腎臓病学会雑誌. 9. 88-94 (1996)
Yuji Morimoto:“针对人肾小球上皮细胞的单克隆抗体 1F3:肾上皮细胞损伤的新标记”日本小儿肾病学会杂志 9. 88-94 (1996)。
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Morimoto Y et al.: "A monoclonal antibody against human epithelial cells (in japanese)" Jap.J.pediat.Nephrol.88. 222-228 (1996)
Morimoto Y 等人:“针对人上皮细胞的单克隆抗体(日语)”Jap.J.pediat.Nephrol.88。
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    0
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Shoji Kagami: "A monoclonal antibody(1F3)to human glomerular eqithelial cells:A new marker to renal epithelial cell injury" Nephron. 75(1). 65-71 (1997)
Shoji Kagami:“针对人肾小球贴皮细胞的单克隆抗体(1F3):肾上皮细胞损伤的新标记”肾单位。
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    0
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Kagami S et al.: "A monoclonal Antibody (1F3) to Human Glomerular Epithelial Cells : A new Marker for Renal Epithelial Cell Injury" Nephron. 75. 65-71 (1997)
Kagami S 等人:“针对人肾小球上皮细胞的单克隆抗体 (1F3):肾上皮细胞损伤的新标记” 肾单位。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Shoji Kagami: "A monoclonal antibody(1F3)to human glomerular epithelial cells:A new marker to renal epithelial cell injury" Nephron. 75(1). 65-71 (1997)
Shoji Kagami:“针对人肾小球上皮细胞的单克隆抗体(1F3):肾上皮细胞损伤的新标记物” Nephron。
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    0
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KAGAMI Shoji其他文献

KAGAMI Shoji的其他文献

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{{ truncateString('KAGAMI Shoji', 18)}}的其他基金

Role of ADAM17 in the progression of chronic kidney disease
ADAM17 在慢性肾脏病进展中的作用
  • 批准号:
    17K10144
  • 财政年份:
    2017
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analyzing glomerular renin angiotensin system with peptidomics for development of therapeutic and diagnostic methods inhibiting disease progression of chronic kidney disease
利用肽组学分析肾小球肾素血管紧张素系统,以开发抑制慢性肾病疾病进展的治疗和诊断方法
  • 批准号:
    26461614
  • 财政年份:
    2014
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The role of angiopoietin-like protein 4 (ANGPTL4) in the glomerular endothelial cell injury and progressive glomerular injury
血管生成素样蛋白4(ANGPTL4)在肾小球内皮细胞损伤及进行性肾小球损伤中的作用
  • 批准号:
    23591570
  • 财政年份:
    2011
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The role of reactive oxygen species (ROS) in the progressive kidney disease in children, and therapeutic strategy based on its mechanisms.
活性氧(ROS)在儿童进行性肾病中的作用以及基于其机制的治疗策略。
  • 批准号:
    20591278
  • 财政年份:
    2008
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation on the Role of ROS in Integrin-Mediated Signaling of Progressive Glomerulonephritis
ROS 在整合素介导的进行性肾小球肾炎信号传导中的作用研究
  • 批准号:
    18591189
  • 财政年份:
    2006
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation for the role of collagen-binding integrin receptor in the development of renal fibrosis
胶原结合整合素受体在肾纤维化发展中的作用研究
  • 批准号:
    14570748
  • 财政年份:
    2002
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation of Integrin-mediated MAP kinase Signaling in Glomerulonephritis
肾小球肾炎中整合素介导的 MAP 激酶信号传导的研究
  • 批准号:
    12670756
  • 财政年份:
    2000
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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