The role of reactive oxygen species (ROS) in the progressive kidney disease in children, and therapeutic strategy based on its mechanisms.

活性氧（ROS）在儿童进行性肾病中的作用以及基于其机制的治疗策略。

• 批准号: 20591278
• 负责人: KAGAMI Shoji
• 金额: $ 2.91万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591278/
• 关键词: 小児腎・泌尿器学; メサンギウム細胞; ポドサイト; ボウマン嚢上皮細胞; 半月体; 活性酸素; NAD(P)Hオキシダーゼ; 腎炎進行; 腎不全; ボウマン襄上皮細胞

The role of reactive oxygen species for cell phenotypic changes was investigated in glomerulonephritis (GN). Increased ROS production was observed in crescents of GN. The increased ROS production was associated with enhanced Nox2 (an NADPH component) expression and parietal epithelial cell proliferation. We also observed the expressions of angiotensin II and its receptor. In addition, cultured glomerular cells stimulated with angiotensin II and H2O2 showed the enhanced phosphorylation of Erk5, a ROS-related MAPK family. Inhibition with siRNA showed that ROS-MAP kinase signaling pathway might enhance cell potential to survive, and thereby play a role in progression of glomerulonephritis.

研究了活性氧在肾小球肾炎（GN）细胞表型改变中的作用。在GN月牙中观察到ROS的产生增加。ROS生成增加与Nox2 （NADPH成分）表达增强和壁上皮细胞增殖有关。同时观察血管紧张素II及其受体的表达。此外，血管紧张素II和H2O2刺激培养的肾小球细胞显示ros相关的MAPK家族Erk5的磷酸化增强。siRNA抑制表明ROS-MAP激酶信号通路可能增强细胞的生存潜能，从而在肾小球肾炎的进展中发挥作用。

项目成果

Low-density lipoprotein apheresis in a pediatric patient with refractory nephrotic syndrome due to focal segmental glomerulosclerosis

局灶节段性肾小球硬化所致难治性肾病综合征儿科患者的低密度脂蛋白单采术

• DOI: 10.1007/s00540-008-0726-z
• 发表时间: 2009
• 期刊: Journal of Anesthesia
• 影响因子: 2.8
• 作者: J. Oto;K. Suga;S. Matsuura;Shuji Kondo;Y. Ohnishi;D. Inui;H. Imanaka;S. Kagami;M. Nishimura
• 通讯作者: M. Nishimura

Enhanced expression of Hydrogen peroxide-inducible clone-5 (Hic-5) on activated mesangial cells in human glomerulonephritis.

人肾小球肾炎激活的系膜细胞上过氧化氢诱导克隆 5 (Hic-5) 的表达增强。

• 发表时间: 2008
• 作者: Suka K;Kondo S;Matsuura S;Kinoshita Y;Urushihara M;Tamaki T;Kagami S
• 通讯作者: Kagami S

Steroid-resistant nephrotic syndrome.

• DOI: 10.1038/ki.2008.297
• 发表时间: 2008-11
• 期刊: Kidney international
• 影响因子: 19.6
• 作者: A. Kitamura;H. Tsukaguchi;K. Maruyama;A. Shono;K. Iijima;S. Kagami;T. Doi

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Enhanced expression of Hic-5 is involved in the development of human and rat mesangioproliferative glomerulonephritis.

Hic-5 表达增强参与人和大鼠系膜增生性肾小球肾炎的发展。

• 发表时间: 2010
• 作者: Kondo S;Suga K;Matsuura S;Kinoshita Y;Urushihara M;Tamaki T;Kagami S
• 通讯作者: Kagami S