Expression and Abnormalities of Keratinocyte Terminal Differentiation-Associated Proteins

角质形成细胞终末分化相关蛋白的表达及异常

基本信息

  • 批准号:
    08670940
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

Immunoelectron microscopic analysis of the process of cornified cell envelope formation in normal epidermis showed that involucrin is cross-linked earlier than loricrin (J Histochem Cytochem, 44 : 167-175,1996). Furthermore, we showed that cornified cell envelopes in psoriatic epidermis without granular layr was rich in involucrin and formed in a premature stage, while psoriatic epidermis with granular layr had envelopes similar to normal (J Histochem Cytochem, 44 : 167-175,1996). We also showed that psoriatic epidermis can be classified into two types ; epidermis with granular layr and without granular layr and these two have different proliferative situation when assessed by morphometry (Br J Dermatol 135 : 433-438,1996. J Invest Dermatol, 109 : 806-810,1997).We induced expression of involucrin and SPRR in cultured normal human keratinocytes by increasing calcium concentration in the culture medium, and analyzed their localization and ultrastructural changes and showed that involucri … More n and SPRR were expressed and incorporated into the cornified cell envelopes in this order (J Invest Dermatol, 108 : 12-16,1997).When we assessed expression patterns of keratinocytes differentiation-related proteins, we found that profilaggrin and trichohyalin had distinct expression patterns in normal and psoriatic epidermis, tongue epithelia and cultured keratinocytes despite that they have similar gene structures (Br J Dermatol, 137 : 9-16,1997). We further revealed that involucrin gene expression is stimulated by alpha and eta isoforms of protein kinase C (J Invest Dermatol, in press).We showed that genetic keratinization disorders, Vohwinkel syndrome and progressive symmetric erythrokeratoderma were diseases of loricrin by morphological and molecular genetic studies (Nature Genetics, 13 : 70-77,1996. J Invest Dermatol, 109 : 604-610,1997, Am J Hum Genet, 61 : 581-589,1997) and we proposed that these diseases can be called loricrin keratoderma (Histol & Histopath, in press. Exp Dermatol, in press).In the analysis of cornified cell envelope formation, disribution of envelope precursor protein, and morphology and function of epidermis in knockout mice for the transglutaminase 1 gene, it was found that cornified cell envelope formation, and distribution and cross-linking of loricrin and profilaggrin were disrupted, barrier function of the epidermis was severely decreased and mice died soon after birth (PNAS,in press). Less
对正常表皮中皮质细胞包膜形成过程的免疫电镜分析表明,外皮蛋白比兜甲蛋白更早交联(J Histochem Cytochem,44:167- 175,1996)。此外,我们还发现,无颗粒层的银屑病表皮中的皮质细胞包膜富含外皮蛋白,并且在早期形成,而有颗粒层的银屑病表皮中的皮质细胞包膜与正常表皮相似(J Histochem Cytochem,44:167- 175,1996)。我们还发现银屑病表皮可分为两种类型:有颗粒层和无颗粒层的表皮,当通过形态计量学评估时,这两种表皮具有不同的增殖情况(Br J Dermatol 135:433- 438,1996. JInvest Dermatol,109:806- 810,1997)。我们通过增加培养液中的钙浓度诱导培养的正常人角质形成细胞中外皮蛋白和SPRR的表达,并分析它们的定位和超微结构变化,结果表明外皮蛋白和SPRR在角质形成细胞中的表达与细胞增殖和分化有关。 ...更多信息 n和SPRR依次表达并整合到皮层细胞膜中(J Invest Dermatol,108:12- 16,1997)。当我们评估角化细胞分化相关蛋白的表达模式时,我们发现聚丝蛋白原和透明质酸在正常和银屑病表皮中具有不同的表达模式,舌上皮细胞和培养的角质形成细胞,尽管它们具有相似的基因结构(BrJDermatol,137:9- 16,1997)。我们进一步揭示了外皮蛋白基因表达受蛋白激酶C的α和η同种型的刺激(J Invest Dermatol,出版中)。我们通过形态学和分子遗传学研究表明,遗传性角化障碍、Vohwinkel综合征和进行性对称性红斑角化病是外皮蛋白的疾病(Nature Genetics,13:70- 77,1996)。J Invest Dermatol,109:604- 610,1997,Am J Genet,61:581- 589,1997),我们提出这些疾病可称为兜甲蛋白角化病(Hierarchy & Histopath,出版中。通过对转谷氨酰胺酶1基因敲除小鼠表皮细胞被膜形成、被膜前体蛋白分布、表皮形态和功能的分析,发现敲除小鼠表皮细胞被膜形成、兜甲蛋白和聚丝蛋白原的分布和交联被破坏,表皮屏障功能严重下降,小鼠出生后很快死亡(PNAS,in press).少

项目成果

期刊论文数量(53)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
K.Tamai: "Compound heterozygosity for a nonsense mutation and a splice site mutation in the type VII collagen gene (COL7A1) in recessive dystrophic epidermolysis bullosa." Laboratory Investigation. 76. 209-217 (1997)
K.Tamai:“隐性营养不良性大疱性表皮松解症中 VII 型胶原基因 (COL7A1) 的无义突变和剪接位点突变的复合杂合性。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
A.Ishida-Yamamoto: "Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentiation" Br J Dermatol. 137. 9-19 (1997)
A.Ishida-Yamamoto:“丝聚合蛋白和毛透明蛋白在上皮分化的各种途径中的独特表达”Br J Dermatol。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
A.Ishida-Yamamoto et al.: "Immunoelectron microscopic analysis of cornified cell envelope formation in normal and psoriatic epidermis." J Histochem Cytochem. 44. 167-175 (1996)
A.Ishida-Yamamoto 等人:“正常和银屑病表皮角化细胞包膜形成的免疫电子显微镜分析。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
A.Ishida-Yamamoto, et al.: "Eccrin syringofibroadenoma (Mascaro). An ultrastructural and immunohistochemical study." Am J Dermatopathol. 18. 207-211 (1996)
A.Ishida-Yamamoto 等人:“Eccrin 汗管纤维腺瘤(Mascaro)。一项超微结构和免疫组织化学研究。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Akemi Ishida-Yamamoto: "Involucrin and SPRR are synthesized sequentially in differentiating cultured epidermal cells" Journol of Investigative Dermatology. 108. 12-16 (1997)
Akemi Ishida-Yamamoto:“外皮蛋白和 SPRR 在分化培养的表皮细胞中依次合成”《皮肤病学研究杂志》。
  • DOI:
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  • 影响因子:
    0
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YAMAMOTO Akemi其他文献

YAMAMOTO Akemi的其他文献

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{{ truncateString('YAMAMOTO Akemi', 18)}}的其他基金

Pathological mechanisms of keratinization abnormalities
角化异常的病理机制
  • 批准号:
    19591294
  • 财政年份:
    2007
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of cellular molecules associated with keratinocyte differentiation
与角质形成细胞分化相关的细胞分子分析
  • 批准号:
    14570794
  • 财政年份:
    2002
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Cell biological analyses of cornified cell envelope formation
角质化细胞包膜形成的细胞生物学分析
  • 批准号:
    10470184
  • 财政年份:
    1998
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).

相似海外基金

Changes in the Cornified Cell Envelope in HPV Infection
HPV 感染时角质化细胞包膜的变化
  • 批准号:
    6721519
  • 财政年份:
    2003
  • 资助金额:
    $ 1.41万
  • 项目类别:
Changes in the Cornified Cell Envelope in HPV Infection
HPV 感染时角质化细胞包膜的变化
  • 批准号:
    6607905
  • 财政年份:
    2003
  • 资助金额:
    $ 1.41万
  • 项目类别:
Changes in the Cornified Cell Envelope in HPV Infection
HPV 感染时角质化细胞包膜的变化
  • 批准号:
    7047882
  • 财政年份:
    2003
  • 资助金额:
    $ 1.41万
  • 项目类别:
Changes in the Cornified Cell Envelope in HPV Infection
HPV 感染时角质化细胞包膜的变化
  • 批准号:
    6857123
  • 财政年份:
    2003
  • 资助金额:
    $ 1.41万
  • 项目类别:
ASSEMBLY OF CORNIFIED CELL ENVELOPE IN TERMINALLY DIFFERENTIATED KERATINOCYTES
终末分化角质形成细胞中角化细胞包膜的组装
  • 批准号:
    6444700
  • 财政年份:
    2001
  • 资助金额:
    $ 1.41万
  • 项目类别:
ASSEMBLY OF CORNIFIED CELL ENVELOPE IN TERMINALLY DIFFERENTIATED KERATINOCYTES
终末分化角质形成细胞中角化细胞包膜的组装
  • 批准号:
    6308948
  • 财政年份:
    2000
  • 资助金额:
    $ 1.41万
  • 项目类别:
Studies on roles of malformation of the cornified cell envelope in phathogenesis of severe ichthyoses
角质化细胞膜畸形在严重鱼鳞病发病中作用的研究
  • 批准号:
    12670839
  • 财政年份:
    2000
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
CORNIFIED CELL ENVELOPE ASSEMBLY IN TERMINALLY DIFFERENTIATED KERATINOCYTES
终末分化角质细胞中的角质化细胞包膜组装
  • 批准号:
    6120575
  • 财政年份:
    1999
  • 资助金额:
    $ 1.41万
  • 项目类别:
Cell biological analyses of cornified cell envelope formation
角质化细胞包膜形成的细胞生物学分析
  • 批准号:
    10470184
  • 财政年份:
    1998
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
ASSEMBLY OF CORNIFIED CELL ENVELOPE IN TERMINALLY DIFFERENTIATED KERATINOCYTES
终末分化角质形成细胞中角化细胞包膜的组装
  • 批准号:
    6281345
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    1998
  • 资助金额:
    $ 1.41万
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