ASSEMBLY OF CORNIFIED CELL ENVELOPE IN TERMINALLY DIFFERENTIATED KERATINOCYTES

终末分化角质形成细胞中角化细胞包膜的组装

• 批准号: 6281345
• 负责人: IICHAL JARNIK
• 金额: $ 0.41万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281345
• 关键词: Mammalia; biomedical resource; growth /development; human tissue; skin

Terminal differentiation of mammalian epidermal keratinocytes eventually creates a keratin filament matrix encased within a sheet of covalently cross-linked proteins called the cornified cell envelope (CE). Biogenesis of the CE is being studied in both normal and diseased states, e.g. psoriasis. - STEM measurements of mass/area on purified CEs allow one to evaluate their homogeneity and test for possible specialized areas within a given CE. CEs isolated from human epidermis (normal and psoriatic), mouse epidermis (normal and transgenic), other epithelia, and cultured keratinocytes are being studied. Several different methods of preparation have been examined since residual detergent between the CE's and the carbon substrate can affect mass measurements. The average thickness and mass/area of the mature envelopes are amazingly the same regardless of the tissue. Tliese results are helping to define the current model of CE assembly.

哺乳动物表皮角质形成细胞的终末分化 最终形成一个包裹在一层 共价交联的蛋白质称为皮质细胞包膜 （CE）。 CE的生物发生正在正常和 疾病状态，例如牛皮癣。 - STEM测量质量/面积 纯化的CE允许人们评估其均匀性并测试其纯度。 在给定CE内的可能的专业领域。 从人中分离的CE 表皮（正常和银屑病），小鼠表皮（正常和银屑病）， 转基因），其他上皮细胞和培养的角质形成细胞正在被 研究了 几种不同的制备方法已经过检验 由于CE和碳基质之间的残留洗涤剂可以 影响质量测量。 的平均厚度和质量/面积， 成熟的包膜不管是什么样的组织都惊人地相同。 这些结果有助于定义CE装配的当前模型。