Molecular Biological Analysis for the Role of Plasminogen Activator in Wound Healings

纤溶酶原激活剂在伤口愈合中作用的分子生物学分析

• 批准号: 08670959
• 负责人: TAKAGI Hajime
• 金额: $ 1.34万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670959/
• 关键词: wound healing; plasminogen activator; laminin; collagen gel; malignant melanoma; ケラチノサイト; プラスミノーゲン・アクチベータ-

Signal transduction mechnisms of cell attachment systems between keratinocytes and basement membranes has not yet elucidated. Seishima M, co-worker, et al. reported the effects of PV (pemphigus vulgaris)-IgG on the keratinocytes such as plasminogen activator. A transient incerase in phospholipase C antivation on a squamous carcinoma cell line, is directly associated with serection of plasminogen activator into the culture medium. Next, we made a model of wound healing to scrape attached cells, and investigated the expression of plasminogen activator , laminin, and cell migration. Also as three-dimentional model, same experiments were. studied on collagen gel culture.As clinical research projects, we investigated the expression of plasminogen activator on two malignant melanoma cells, obtained from operation and inoculated melanoma cell line cells to mice. After apoptosis induction by anti-cancer drugs, we found the expression of plasminogen activator. Though the difference of apoptotic cell expression on treatment were found, we could not see the mudh difference between before and after treatment.These resluts require further investigation. So we will examine the relationship between the expression of such cytoskelton and cell adhesion molecules and tumor metastasis.

角质形成细胞与基底膜之间的细胞附着系统的信号转导机制尚未阐明。Seishima M等报道了寻常型天疱疮(pemphigus vulgaris)-IgG对纤溶酶原激活剂等角质形成细胞的影响。在鳞状癌细胞系上，磷脂酶C抗活性的短暂性增加与选择纤溶酶原激活剂进入培养基直接相关。接下来，我们制作了伤口愈合模型，刮除附着细胞，并研究了纤溶酶原激活剂、层粘连蛋白的表达和细胞迁移。同样作为三维模型，进行了相同的实验。胶原凝胶培养的研究。作为临床研究项目，我们研究了纤溶酶原激活剂在手术获得的两种恶性黑色素瘤细胞和接种于小鼠的黑色素瘤细胞系细胞上的表达。在抗肿瘤药物诱导细胞凋亡后，我们发现了纤溶酶原激活物的表达。虽然发现了治疗前后凋亡细胞表达的差异，但我们没有看到治疗前后的差异。这些结果需要进一步调查。因此，我们将研究这些细胞骨架和细胞粘附分子的表达与肿瘤转移的关系。