Molecular biological analysis for apoptosis induction and variation of cytoskeltons ＆ adhesion molecules in malignant melanoma

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Basic research1. Immunohistochemically we confirmed sequential bioimmunochemotherapy induced apoptosis for mouse melanoma cells. 2. We checked drug sensitivity of cultured human melanoma cells by FACScan. 3. Variation of cytoskelton, especially vimentin, were examined with immunofluorescent technique. But analysis was not enough, because of technical trouble. 4. Animal experiments were not done. We did not prepare to a favorable environment. 5. We compared extent of multidrug-resistance-associated protein between before and after chemotherapy. 6. We have been trying establish cell line of drug resistant melanoma cells.Clinical research1. We refer to the apoptosis cells, apoptosis- related gene/protein, and adhesion molecules by melanoma patients specimens immunohistochemically. 2. We measured melanoma inhibitory activity by ELISA method, and checked with association of the value and clinical course. 3. We evaluated of combination chemotherapy (DAC-Tam) for advanced malignant melanoma.SummaryWe cannot show enough results in this periods, but we got some promising products. We will keep this projects going on some experiments.