Induction of cytotoxic T lyphocytes (CTLs) recognized hepatocellular carcinoma (HCC) from tumor-infiltrating lymphocytes of HCC and identification of the tumor-rejection antigen genes of HCC.

诱导细胞毒性T淋巴细胞（CTL）从HCC的肿瘤浸润淋巴细胞中识别肝细胞癌（HCC），并鉴定HCC的肿瘤排斥抗原基因。

• 批准号: 08671500
• 负责人: SAITSU Hideki
• 金额: $ 1.09万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671500/
• 关键词: HCC; CTL; MHC-nonrestriction; tumor-rejection antgens; 癌退縮抗原

We investigatet T Cell immunity against hepatocellular carcioma (HCC), and showed that both peripheral blood mononuclear cells (PBMC) and tumor-infiltrating lymphocytes (TIL) incubated with ingterleukin-2 alone displayd HHLA-nonrestricted but hepatic cancer-specific cytotoxicity in a majority of parients with HCC.Namely, they lysed both HCC and cholagiocellular carcinomas in an HLA-nonrestricted manner, but not any tumors with the other histological types tested, normal hepatocytes or the cells transfected with Hepatitis C virus or MUCl gene. These CTL lines and clones were phenotypically CD3^+CD4^+CD8^-. These unique CTL could play important riles in T cell immunity against HCC.

我们研究了抗肝细胞癌（HCC）的T细胞免疫，结果表明，在大多数HCC患者中，单独用白细胞介素-2孵育的外周血单个核细胞（PBMC）和肿瘤浸润淋巴细胞（TIL）均表现出HLA非限制性但肝癌特异性的细胞毒性，即它们以HLA非限制性方式溶解HCC和胆管细胞癌，而不是具有所测试的其他组织学类型的任何肿瘤、正常肝细胞或用丙型肝炎病毒或MUC 1基因转染的细胞。这些CTL系和克隆的表型为CD 3 ^+ CD 4 ^+ CD 8 ^-。这些特异性CTL在T细胞抗肝癌免疫中发挥重要作用。

项目成果

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Schichijo, S.et al.：“编码人类鳞状细胞癌抗原肽的基因，可被细胞毒性 T 淋巴细胞识别。”

Uhi,T.et al.: "HLA class I-restricted tumor-specific cytotoxic T lymphocytes from metastatic lymph nodes of esophageal cancers." Cell.Immunol.Vol.177. 137-143 (1997)

Uhi,T.等人：“来自食管癌转移淋巴结的 HLA I 类限制性肿瘤特异性细胞毒性 T 淋巴细胞。”

Hoshino, T.et al.: "Increase of TCRgammadelta^+ T cells in peripheral blood of three patients with adult still's disease." J.Rheumatology. Vol. 23. 124-129 (1996)

Hoshino, T. 等人：“三名成人斯蒂尔病患者外周血中 TCRgammadelta^ T 细胞增加。”

Hoshino, T.et al.: "Increase of TCRγδ^+ T cells in peripheral blood of three patients with adult still's disease." J.Rheumatology. Vol.23. 124-129 (1996)

Hoshino, T. 等人：“三名成人斯蒂尔病患者外周血中 TCRγδ^+ T 细胞的增加”，J.Rheumatology 第 23 卷（1996 年）。

Gohara, R.et al.: "Histocompatibility leukocyte antigen-A2402-restricted cytotoxic T lymphocytes recognizing adenocarcinoma in Tumor-infiltratinglymphocytes of patients with colon cancer." Jpn. J.Cancer Res.Vol. 88. 198-204 (1997)

Gohara, R.等人：“组织相容性白细胞抗原 A2402 限制性细胞毒性 T 淋巴细胞识别结肠癌患者肿瘤浸润淋巴细胞中的腺癌。”