Inductions of myocardial 3-L-Nitrotyrosincin rat cardiac allograft rejection

心肌3-L-硝基酪氨酸诱导大鼠心脏同种异体移植排斥反应

• 批准号: 08671505
• 负责人: OHMI Mikio
• 金额: $ 1.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671505/
• 关键词: cardiactransplantation; acuterejection; 3-L-nitrotyrosine; 冠動脈病変; HSP60; 心臓移植; 冠動脈硬化; ストレス蛋白

Heterotopic abdominal transplantation from Lewis to Fisher rats was used as a model of cardiac allograft rejection. Hearts were immediately removed after reperfusion used as sham control. Rats were sacrificed at 1,3, and 7 days after transplantation (n = 5 at each time point). Syngeneic Lewis to Liews abdominal heart transplant were also performed by the same technique (n = 3 at 1 and 7days). HPLC analysis, immunohistochemistry for nitrotyrosine, and histological studies were performed at each time points. N0_2-Tyr was induced with significant increases by day I compared with sham control (0.049*036% vs 0.000*0.000%, N0_2-Try/Total tyrosine, p<0.001) and continued to increase progressively through days 3(0.1060.011%, p <0.001) and 7 (0.162*0.013%, p <0.001). N02-Tyr immunostainina 01 infiltrating macrophages and cardiac muscle fibers increased significantly in the allograft at days 3 to 7. These results suggested that protein tyrosine nitration by NO plays a role in cardiac allograft rejection after transplantation.

Lewis 大鼠和 Fisher 大鼠的异位腹部移植被用作心脏同种异体移植排斥反应的模型。再灌注后立即取出心脏用作假手术对照。移植后第 1,3 和 7 天处死大鼠（每个时间点 n = 5）。还采用相同的技术进行了同系 Lewis 至 Liews 腹部心脏移植（第 1 天和第 7 天时，n = 3）。在每个时间点进行 HPLC 分析、硝基酪氨酸免疫组织化学和组织学研究。与假手术对照相比，N0_2-Tyr 在第一天诱导显着增加（0.049*036% vs 0.000*0.000%，N0_2-Try/总酪氨酸，p<0.001），并在第 3 天（0.1060.011%，p <0.001）和第 7 天继续逐渐增加（0.162*0.013%，p <0.001）。第3至7天，同种异体移植物中的N02-Tyr免疫染色01浸润巨噬细胞和心肌纤维显着增加。这些结果表明，NO对蛋白质酪氨酸的硝化在移植后同种异体移植物排斥反应中发挥作用。

项目成果

Masahiro Sakurai: "Inductions of myocardial 3-L-Nitrotyrosinein rat cardiac allograft rejection" (Transplantation submitted).

Masahiro Sakurai：“大鼠心脏同种异体移植排斥反应中心肌 3-L-硝基酪氨酸的诱导”（已提交移植）。