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Investigation of the epidermal proteins related with skin barrier function

与皮肤屏障功能相关的表皮蛋白的研究

基本信息

批准号：
08670992
负责人：
TAKAHASHI Masae
金额：
$ 1.22万
依托单位：
Kinki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670992/
关键词：
Phosphorylated cystatin alpha Human bactericidal permeability increaseing protein Lactorerricin B Skin barrier function human bactericidal permeability increasing protein シスタチンA アトピー性皮膚炎生体防御機能皮膚タンパク

项目摘要

A phosphorylated cystatin alpha (P-cystatin alpha), which was a cysteine proteinase inhibitor isolated from newborn rat epidermis, located in keratohyalin granules in the stratum granulosum and the cornified envelope of the stratum corneum. This location suggested that P-cystatin alpha could have barrier function on the skin surface. We repoted that P-cystatin alpha inhibited a cysteine proteinase activity from Staphylococcus aureus V8 and a growth of the bacteria. In this experiment, the inhibitoty properties of P-cystatin alpha and its crosslinked protein with filaggrin linker segment peptide by epidermal transglutaminase (P-cystatin alpha-FLSP) against polio virus were investigated. P-cystatin alpha and P-cystatin alpha-FLSP inhibitied the growth of polio virus and the production of cysteine proteinase from the virus.The antibody against human bactericidal permeability increasing protein reacted positively with the cytoplasm of the inner root sheath by immunohistochemical study and the 55 kDa protein in immunoblotting analysis. The antibody against lactoferricin B reacted with the cytoplasm of the stratum granulosum and the 40 kDa protein. The two proteins inhibited the growth of some bacteria, therefore, the proteins having similar properties of these proteins could exist in the skin and relate with an epidermal barrier function.

磷酸化半胱氨酸蛋白酶抑制剂α（P-cystatin α）是从新生大鼠表皮中分离的半胱氨酸蛋白酶抑制剂，位于角质层的颗粒层和角质层的角质膜中的透明角质颗粒中。该位置表明P-半胱氨酸蛋白酶抑制剂α可能在皮肤表面上具有屏障功能。我们报道P-半胱氨酸蛋白酶抑制剂α抑制金黄色葡萄球菌V8的半胱氨酸蛋白酶活性和细菌生长。本实验研究了P-半胱氨酸蛋白酶抑制剂α（P-cystatinalpha-FLSP）及其与聚丝蛋白连接片段肽（filaggrin linker segment peptide）的交联蛋白（P-cystatinalpha-FLSP）对脊髓灰质炎病毒的抗病毒活性。P-cystatin alpha和P-cystatin alpha-FLSP能抑制脊髓灰质炎病毒的生长和半胱氨酸蛋白酶的产生，免疫组化显示抗人杀菌通透性增加蛋白抗体与脊髓灰质炎内根鞘细胞质呈阳性反应，免疫印迹显示抗人杀菌通透性增加蛋白抗体与脊髓灰质炎内根鞘细胞质呈阳性反应。抗乳铁蛋白B的抗体与颗粒层的细胞质和40 kDa蛋白质反应。这两种蛋白质抑制某些细菌的生长，因此，具有与这些蛋白质相似性质的蛋白质可能存在于皮肤中并与表皮屏障功能有关。