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Radiotoxicity after iodine-131 therapy for thyroid cancer using the micronucleus assay

使用微核测定法检测碘 131 治疗甲状腺癌后的放射毒性

基本信息

批准号：
08671009
负责人：
WATANABE Naoto
金额：
$ 1.54万
依托单位：
Toyama Medical and Pharmaceutical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

radiation damage to lymphocytes after I- 131 therapy using the cytokines is-blocked micronucleus as s a y . The chromosomal damage to lymphocytes induced by I-131 in vivo should result in augmentation of the cells with micronuclei. Methods ; We studied 25 patients with differentiated thyroid carcinoma, who were treated with 3.7 GBq of I- 131. Isolated lymphocytes collected from patients 1 week after therapy were harvested and treated according to the cytokines is-blocked method of Fenech and Morley. Micronuclens number of micronuclei per 500b inucleated cells was scored by visual inspction. As controls, lymphocytes from the same patients before therapy were also studied. In an in vitro study, lymphocytes from three patients at least 3 months after therapy were exposed doses varying from 0.25 to 1 Gy and stud led with the same method. Results : The mean number (mean*SD) of micronuclei after treatment was significantly increased (p<0.05) as comparted to control subjects (15.7* 2.7 vs. 5.4 * 1.4). Since there was an interval ranging from 6 to 20 months (mean 11.8 months) between the present and the last radio iodine therapy, no significant effect on the frequency of micronucleus with cumulative radiation exposure of I- 131 to lymphocytes was detected. Internal radiation absorbed doses estimated for 25 patients were 0.33 0.09 Gy ln this external irradiation study. C o n c I u s i o n s : The relatively low frequency of lymphocyte micronuclei induced by iodine-13 1 in vivo and lack of significant effect on the frequency of lymphocyte micronuclei with cumulative iodine-131 s u p p o r t e d the contention t h a t short-term non-stochastic damage of this therapy with 3 .7 G B q of 1-131 in thyroid cancer patientsis minimal and reversible .

使用细胞因子阻断微核试验进行 I-131 治疗后对淋巴细胞的辐射损伤。 I-131 在体内诱导的淋巴细胞染色体损伤应导致细胞微核增多。方法 ;我们研究了 25 名分化型甲状腺癌患者，他们接受了 3.7 GBq 的 I-131 治疗。治疗后 1 周从患者收集分离的淋巴细胞，并根据 Fenech 和 Morley 的细胞因子阻断方法进行处理。微核通过目视检查对每500b个有核细胞的微核数进行评分。作为对照，还研究了治疗前来自同一患者的淋巴细胞。在一项体外研究中，来自三名患者的淋巴细胞在治疗后至少 3 个月接受了 0.25 至 1 Gy 不等的剂量照射，并用相同的方法进行了引导。结果：与对照受试者相比，治疗后微核的平均数量（平均值*SD）显着增加（p<0.05）（15.7 * 2.7 vs. 5.4 * 1.4）。由于本次放射性碘治疗与最后一次放射性碘治疗之间有6至20个月（平均11.8个月）的间隔，因此检测到I-131累积辐射暴露对淋巴细胞的微核频率没有显着影响。在这项外部照射研究中，25 名患者的内部辐射吸收剂量估计为 0.33-0.09 Gy。结论：体内碘 13 1 诱导的淋巴细胞微核频率相对较低，且对累积碘 131 淋巴细胞微核频率没有显着影响，支持了该疗法对甲状腺癌具有 3 .7 GB q 1-131 短期非随机损伤的论点。患者数量极少且可逆。