Estimation of tumor proliferative activity according to expression of cell-cycle-related antigen and selection of fractionation schema in radiation therapy.

我们对肿瘤增殖和放射治疗进行了各种基础和临床研究。1）为了更好地了解放射治疗（RT）后肿瘤细胞的再增殖动力学，我们研究了小鼠肿瘤单剂量照射后细胞增殖参数的变化。作为结论之一，先前静止的细胞募集到肿瘤中的增殖池中被认为有助于RT后的再增殖。（参考文献2）2）使用小鼠肿瘤研究了抗血管生成剂（TNP-470）与分级RT的联合作用。在空气中进行分次RT时，给予TPN-470降低了肿瘤控制概率。这一意外结果可能与TNP-470部分抑制再氧合有关，因为TNP-470在缺氧条件下对TCD-50无显著影响。（参考文献6）3）评价总治疗时间（OTT）对RT局部控制的影响，以考虑RT中的最佳OTT。 ...更多信息 分析食管癌、声门癌和宫颈癌的晚期毒性。在多因素分析中，OTT是局部控制的重要预后因素。结论：在正常组织和患者耐受范围内，应尽量缩短OTT。（4）研究食管鳞癌放射治疗后增殖细胞核抗原（PCNA）和Ki-67免疫组化指标与局部控制率和生存率的关系。65例食管癌患者在放疗前均行活检。PCNA LI与已知的食管癌局部控制预后因素无关。PCNA LI <50%或Ki-67 LI <45%的患者的局部复发率显著高于LI较高的患者（p<0.05）。结论：PCNA LI和Ki-67 LI与食管局部控制概率显著相关。PCNA LI可作为食管癌放射治疗方案选择的参考指标。少

We have performed various basic and clinical studies on tumor proliferation and radiation therapy,1)To understand better the repopulation kinetics of tumor cells after radiotherapy (RT), we investigated changes in cell proliferative parameters after single-dose irradiation of murine tumors. As one of conclusions, recruitment of previously quiescent cells into the proliferative pool in the tumors was suggested to contribute to repopulation after RT.(ref.2)2)Combined effects of an antiangiogenic agent (TNP-470) with fractionated RT were investigated using a murine tumor. The tumor control probability decreased by administrating TPN-470 during fractionated RT in air. This unexpected result may be contributable to partial inhibition of reoxygenation by TNP-470, because no significant changes in TCD-50 under hypoxic conditions by TNP-470. (ref.6)3)The effects of overall treatment time (OTT) on local control of RT were evaluated to consider optimal OTT in RT.Local control rates and acute and … More late toxicities of esophageal cancer, glottic cancer, and uterine cervical cancer were analyzed. In the multivariate analysis, OTT was a significant prognostic factor for local control. In conclusion, OTT should be kept as short as Possible wiyhin the limits of tolerance of normal tissues and patients. (ref.4)4)The relationship of immunohistochemical indices of proliferating cell nuclear antigen (PCNA) and Ki-67 to local control and survival rates for patients with esophageal squamous cell carcinomas treated by definitive radiotherapy (RT) was investigated. Biopsy materials before RT were obtained from 65 patients with esophageal cancer. The PCNA LI was independent of known prognostic factors on local control for esophageal cancer. Patients with the PCNA LI of<50% or the Ki-67 LI of<45% showed significantly higher local recurrence rates than those with higher LIs (p<0.05). In conclusion, PCNA LI and Ki-67 LI were significantly correlated with local control probabilities in esophageal. squamous cell carcinomas treated by definitive RT.PCNA LI may be a useful parameter for selecting the fractionation scheme of esophageal cancers. Less