beta3 adrenergic receptor gene polymorphism and PC-1 activity in obese NIDDM patients

肥胖NIDDM患者β3肾上腺素受体基因多态性和PC-1活性

• 批准号: 08671188
• 负责人: IWAMOTO Yasuhiko
• 金额: $ 1.09万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671188/
• 关键词: NIDDM; beta3 adrenergic receptor gene; insulin resistance; PC-1; leptin

1. The corre1ations between the polymorphism of beta3 adrenergic receptor(AR) gene(trp64Arg) and various clinical manifestations were studied in NIDDM patients. The prevalences of wild type of beta3 AR gene (TT), Trp64Arg heterozygotes (TA) and homozygotes (AA) were 69%, 25% and 6%, respectively. There was no difference in BMI, max BMI in the past and onset age of diabetes among 3 groups. There was no difference in serum leptin level and PAI-1 activity among 3 groups.2. The contribution of the beta3 AR gene polymorphism to body weight gain during pregnancy was studied in NIDDM women. Excess weight gain during pregnancy, as defined by maximum BMI during pregnancy minus basal BMI before pregnancy exceeding 5, was observed in 12.2% of the wild type (TT), 19.2% of heterozygotes and 28.6% of homozygotes, suggesting that homozygous (AA) subjects with NIDDM tended to show excess weight gain during pregnancy.3. The activities of tyrosin kinase inhibitor PC-1 in cultured dermal fibroblasts obtained from NIDDM patients were compared to those of control subjects. The PC-1 activity of the NIDDM patients was 85.2*33.lnmol/mg/min (n=17, mean*SD) and significantly higher than that of control (46.2*12.7, n=7). In additions, the PC-1 activity of a patient with Werner's syndrome with insulin resistance was found to be increased.

1.本文研究了β_3肾上腺素能受体（AR）基因（trp 64 Arg）多态性与NIDDM患者各种临床表现的关系。β 3 AR基因野生型（TT）、Trp 64 Arg杂合子（TA）和纯合子（AA）的检出率分别为69%、25%和6%。3组患者的BMI、既往最大BMI、糖尿病发病年龄无差异。3组间血清瘦素水平和派-1活性无差异.在NIDDM妇女中研究β 3 AR基因多态性对妊娠期体重增加的贡献。妊娠期间体重增加过多（定义为妊娠期间最大BMI减去妊娠前基础BMI超过5）在12.2%的野生型（TT）、19.2%的杂合子和28.6%的纯合子中观察到，表明纯合子（AA）NIDDM受试者倾向于在妊娠期间表现出体重增加过多。酪氨酸激酶抑制剂PC-1的活动，在培养的皮肤成纤维细胞从NIDDM患者进行了比较，对照组。NIDDM患者PC-1活性为85.2 × 33.1nmol/mg/min（n=17，mean × SD），明显高于正常对照组（46.2 × 12.7，n=7）。此外，发现伴有胰岛素抵抗的Werner综合征患者的PC-1活性增加。

项目成果

手納信一: "膜糖蛋白PC-1とインスリン抵抗性" 日本臨牀(1997年増刊号). 55. 812-816 (1997)

Shinichi Teno：“膜糖蛋白 PC-1 和胰岛素抵抗”Nippon Rinsho (1997 年特刊) 55. 812-816 (1997)。

Teno S, Iwasaki N, Kanno H, Sanaka M, Minei S, Kanamuro R, Omori Y, Iwamoto Y: "Polymorphism of the beta3 adrenergic receptor gene and weight gain in diabetes women" Diabetes Res Clin Pract. (in press). (1999)

Teno S、Iwasaki N、Kanno H、Sanaka M、Minei S、Kanamuro R、Omori Y、Iwamoto Y：“β3 肾上腺素受体基因的多态性和糖尿病女性体重增加”糖尿病研究临床实践。

手納信一: "Werner Syndromeにおける遺伝子変異の同定とチロシンキナーゼ阻害物質PC-1活性の測定" 日本臨床代謝学会記録. 34. (1997)

Shinichi Teno：“维尔纳综合征基因突变的鉴定和酪氨酸激酶抑制剂PC-1活性的测量”日本临床代谢学会记录34。（1997）。

手納信一: "Werner Syndromeにおける遺伝子変異の同定とチロシンキナーゼ阻害物質PC-1活性の測定" 日本臨床代謝学会記録. 34. 38 (1997)

Shinichi Teno：“维尔纳综合征基因突变的鉴定和酪氨酸激酶抑制剂 PC-1 活性的测量”日本临床代谢学会记录 34. 38 (1997)。

〓nagisawa,K: "Polymorphism of the β3-adrenergic receptor gene and weight gain in diabetic women" Diabetes Res Clin Pract. 印刷中. (1999)

〓nagisawa, K：“糖尿病女性中 β3 肾上腺素受体基因的多态性和体重增加”，糖尿病研究临床实践 (Diabetes Res Clin Pract)（1999 年）。