NON-DESTRUCTIVE METABOLIC ANANLYSIS OF TRANSPLANTED ORGANS USING MULTI-NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

使用多核磁共振波谱对移植器官进行非破坏性代谢分析

• 批准号: 08671326
• 负责人: MUNAKATA Tahashi
• 金额: $ 1.15万
• 依托单位: ASAHIKAWA MEDICAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671326/
• 关键词: 31P MR SPECTROSCOPY; 1H MR SPECTROSCOPY; PRESERVED LIVER; IN VIVO MEASUREMENT; MULTI-NUCLEAR MRS; RELAXATION TIME; INTRACELLULAR pH; LIVER TRANSPLANTATION; サーフェイスコイル法

In the first year, we analyzed the metabolism of preserved liver. The recovery of high energy metabolites after reperfusion became lower according to the preservation time. Intracellular pH of the preserving liver changed to accidosis with the accumulation of lactic acid. The relaxation time measured by 1H-MRS of the liver preserved in Krebs-Henseleit Medium prolonged according to the preservation time (pre-preservation 274.5(]SY.+-。[)21.3ms, 24hr preservation 402.6(]SY.+-。[)35.2ms). On the other hand, that of the liver preserved in UW solution slightly shortened until 12hr and it was still constant after 12hr (pre-preservation 254.5(]SY.+-。[)21.3ms, 12hr preservation 242.6(]SY.+-。[)31.8ms). The prolongation of the relaxation time was correlated with the water volume (g%) of the preserved liver. The relationship between the changes in relaxation time and the intracellular edema of the preserved liver. From these results, we confirmed the usefulness of the multi-nuclear magnetic resonance spectroscopy for the non-invasive observation of changes in energy metabolism, intracellular pH and relaxation time during preservation.In the second year, we investigated the methods for obtaining the in vivo MRS of the from body surface which supposed the transplanted liver. We clarified that 80% or more of the components in the obtained MR spectra through the abdominal wall was originated from the rat liver by using surface coil methods. The beta-ATP peak decreased after the portal vein occlusion and it became almost constant at 20% of its initial level after 30 minutes. We applied this method to the transplanted rat livers. Unfortunately the data were not stable because of the complicated technique of the rat liver transplantation.However from the results obtained in these two years, we confirmed the efficacy of the multinuclear magnetic resonance spectroscopy as the non-invasive monitor of transplanted organs before and after transplantation.

在第一年，我们分析了保存肝脏的代谢。随着保存时间的延长，再灌注后高能代谢产物的回收率逐渐降低。随着乳酸的积累，保存肝脏细胞内pH值向酸性转变。保存在Krebs-Henseleit介质中的肝脏，1H-MRS测量的弛豫时间随保存时间延长而延长（保存前274.5（± SD））。[] 21.3ms，24小时保存402.6 ± 0.01。[）35.2ms）。而UW液保存的肝脏，其酶活在12小时前略有缩短，12小时后仍保持不变（保存前为254.5 ± 0.01）。[] 21.3ms，12小时保存242.6（±）。[）31.8ms）。弛豫时间的延长与保存肝脏的含水量（g%）相关。保存肝脏弛豫时间的变化与细胞内水肿的关系。从这些结果中，我们证实了多核磁共振波谱的有用性的能量代谢，细胞内的pH值和弛豫时间的变化，在保存过程中的非侵入性观察。在第二年，我们探讨了从身体表面，假设移植肝脏的体内MRS的方法。我们澄清，80%或更多的成分，在所获得的MR光谱通过腹壁起源于大鼠肝脏，通过使用表面线圈的方法。门静脉阻断后β-ATP峰值下降，30分钟后几乎稳定在初始水平的20%。我们将此方法应用于大鼠肝移植。但由于大鼠肝移植技术复杂，数据不稳定，但从这两年的结果来看，我们证实了多核磁共振波谱作为移植器官移植前后无创监测的有效性。

项目成果

I.TOMITA,M.SAWA,T.MUNAKATA,K.TANAKA AND S.KASAI: "The benefical effects of dibutyryl cyclic adenosine monophosphate on warm ischemic injury of the liver induced by cardiac arrest." TRANSPLANTATION. Vol, 62. 167-173 (1996)

I.TOMITA、M.SAWA、T.MUNAKATA、K.TANAKA 和 S.KASAI：“二丁酰环单磷酸腺苷对心脏骤停引起的肝脏热缺血性损伤的有益作用。”

Y.NISHIDA,K.TANAKA,T.MUNAKATA,AND S.KASAI: "Evaluation of functional recovery of regenerating rat liver after partial hepatectomy using 31P-NMR spectroscopy." JOURNAL OF SURGICAL RESERACH. Vol, 62. 385-390 (1996)

Y.NISHIDA、K.TANAKA、T.MUNAKATA 和 S.KASAI：“使用 31P-NMR 波谱法评估部分肝切除术后大鼠肝脏再生的功能恢复。”

Y.NISHIDA: "Evaluation of functional recovery of regenerating rat liver after partial hepatectomy using 31P-NMR spectroscopy" Journal of Surgical Research. 61・2. 385-390 (1996)

Y.NISHIDA：“使用 31P-NMR 光谱评估部分肝切除术后再生大鼠肝脏的功能恢复”，外科研究杂志 61・2 385-390（1996）。