NON-DESTRUCTIVE METABOLIC ANANLYSIS OF TRANSPLANTED ORGANS USING MULTI-NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

第一年，我们分析了保存肝脏的代谢。随着保存时间的延长，再灌注后高能量代谢物的恢复逐渐降低。保存肝的细胞内pH随乳酸的积累而改变为意外。在Krebs-Henseleit培养基中保存的肝脏1H-MRS松弛时间根据保存时间延长(保存前274.5（]SY.+- . [)21.3ms，保存24小时402.6(]SY.+- . [)35.2ms）。而在UW溶液中保存的肝脏，在12h前略有缩短，12h后仍保持不变(保存前254.5（]SY.+- . [)21.3ms，保存12小时242.6(]SY.+- . [)31.8ms）。松弛时间的延长与保存肝脏的含水量（g%）有关。松弛时间变化与保存肝细胞内水肿的关系。从这些结果，我们证实了多核磁共振波谱在无创观察保存过程中能量代谢、细胞内pH和松弛时间的变化方面的有用性。第二年，我们研究了从体表获得假定移植肝脏的活体磁共振成像的方法。通过表面线圈法，我们明确了通过腹壁获得的MR光谱中80%或更多的成分来自大鼠肝脏。门静脉阻断后β - atp峰值下降，30分钟后基本稳定在初始水平的20%。我们将这种方法应用于移植的大鼠肝脏。不幸的是，由于大鼠肝移植技术复杂，数据不稳定。然而，从这两年获得的结果来看，我们证实了多核磁共振波谱作为移植器官移植前后无创监测的有效性。

In the first year, we analyzed the metabolism of preserved liver. The recovery of high energy metabolites after reperfusion became lower according to the preservation time. Intracellular pH of the preserving liver changed to accidosis with the accumulation of lactic acid. The relaxation time measured by 1H-MRS of the liver preserved in Krebs-Henseleit Medium prolonged according to the preservation time (pre-preservation 274.5(]SY.+-。[)21.3ms, 24hr preservation 402.6(]SY.+-。[)35.2ms). On the other hand, that of the liver preserved in UW solution slightly shortened until 12hr and it was still constant after 12hr (pre-preservation 254.5(]SY.+-。[)21.3ms, 12hr preservation 242.6(]SY.+-。[)31.8ms). The prolongation of the relaxation time was correlated with the water volume (g%) of the preserved liver. The relationship between the changes in relaxation time and the intracellular edema of the preserved liver. From these results, we confirmed the usefulness of the multi-nuclear magnetic resonance spectroscopy for the non-invasive observation of changes in energy metabolism, intracellular pH and relaxation time during preservation.In the second year, we investigated the methods for obtaining the in vivo MRS of the from body surface which supposed the transplanted liver. We clarified that 80% or more of the components in the obtained MR spectra through the abdominal wall was originated from the rat liver by using surface coil methods. The beta-ATP peak decreased after the portal vein occlusion and it became almost constant at 20% of its initial level after 30 minutes. We applied this method to the transplanted rat livers. Unfortunately the data were not stable because of the complicated technique of the rat liver transplantation.However from the results obtained in these two years, we confirmed the efficacy of the multinuclear magnetic resonance spectroscopy as the non-invasive monitor of transplanted organs before and after transplantation.