METABOLIC RESPONSES TO SIV INFECTION REVEALED BY IN VIVO ^1H MR SPECTROSCOPY

体内 ^1H MR 光谱揭示了对 SIV 感染的代谢反应

• 批准号: 7349117
• 负责人: Eva-Maria Ratai
• 金额: $ 6.54万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349117
• 关键词: METABOLIC; RESPONSES; SIV; INFECTION; REVEALED

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In vivo ^1H magnetic resonance spectroscopy (MRS) studies of HIV-infected humans have demonstrated significant metabolic abnormalities that vary by brain region and are poorly understood. Metabolic changes in the frontal cortex, basal ganglia and white matter in 15 SIV-infected macaques were investigated using in vivo 1H MRS during the first month of infection. The most significant abnormalities were observed at the time of peak viremia, at 2 weeks post infection (p.i.). Changes in the N-acetylaspartate (NAA), ¿choline¿ (Cho) and myo-inositol (MI) resonances were found relative to the creatine (Cr) resonance and in absolute terms. Changes in Cho and Cho/Cr were most similar across the brain regions, increasing at 2 weeks p.i., and falling below baseline levels at 4 weeks. Complex regional variations in MI/Cr ratios were observed over time. Most interesting were the regional differences in NAA. Significant decreases in NAA and NAA/Cr, reflecting neuronal injury, were observed only in the frontal cortex at the time of peak viremia. While there are several potential mechanisms to explain these results, the data best support the hypothesis that different brain regions have variable intrinsic vulnerabilities to neuronal injury caused by the AIDS virus.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在活体中，HIV感染者的1H磁共振波谱(MRS)研究显示了明显的代谢异常，这些异常因大脑区域而异，但人们对此知之甚少。用活体~1H MRS研究了15只SIV感染猕猴额叶皮质、基底节和白质在感染后1个月的代谢变化。最显著的异常出现在病毒血症高峰期，即感染后2周。N-乙酰天冬氨酸(NAA)、胆碱(CHO)和肌醇(MI)的共振相对于肌酸(Cr)共振和绝对值的变化。Cho和Cho/Cr的变化在不同脑区的变化最为相似，在P.I.2周时增加，4周时降至基线水平以下。随着时间的推移，MI/Cr比值出现了复杂的区域性变化。最有趣的是NAA的地区差异。反映神经元损伤的NAA和NAA/Cr仅在病毒血症高峰期的额叶皮质观察到显著下降。虽然有几种可能的机制来解释这些结果，但数据最好地支持了这样的假设，即不同的大脑区域对艾滋病病毒引起的神经元损伤具有不同的内在脆弱性。