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Experimental studies to develope a new treatment for the organ transplanataion by the inducing prolonged immuno tolerance and chimerism

通过诱导长期免疫耐受和嵌合来开发器官移植新疗法的实验研究

基本信息

批准号：
08671348
负责人：
TAKANO Kunio
金额：
$ 1.47万
依托单位：
Yamanashi Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

Despite recent developments in immunosuppressive agents, an ideal treatment is still needed for successful intestinal transplantation. The induction of specific tolerance of allograft without the need for prolong immunosupperssion or extensive recipient pretreatment will be expected to improve the result of intestinal transplantation and to reduce the complications. The aim of our study was to clarify the effects of splenotransplantation from mother to child on the induction of prolonged specific immuno-tolerance to living related intestinal transplantation using a rat model.From our results, we found that the transplanted spleen well grew up in child omentum more that adult one's and suspected that the child spleen played to reject the transplanted spleen from mother. We speculated that a combination of extirpation of child spleen and transplantation of a piece of spleen might induce prolonged immunotolerance to living-related organ transplantation. Further experiments are necessary to evaluate the proper timing of splenectomy and splenotransplantation and to clarify the effects of the transplanted spleen from mother to child on inducing specific immunotolerance in a animal model using not only skin transplantation but also organ transplantation as the second transplantation. A combination of recipient splenectomy and splenotransplantaion from mother to child (recipient) might induce prolonged immunotolerance to living-related intestinal transplantation.

尽管最近免疫抑制剂的发展，一个理想的治疗仍然需要成功的肠移植。在不需要长期免疫抑制或广泛的预处理的情况下诱导同种异体肠移植的特异性免疫耐受，有望改善肠移植的效果，减少并发症。本研究采用大鼠活体小肠移植模型，观察了母子脾移植对诱导长期特异性免疫耐受的影响，结果发现，移植脾在儿童网膜中生长良好，而在成人网膜中生长较好，这可能是儿童脾对母脾移植产生排斥反应的原因之一。我们推测，儿童脾脏摘除和脾脏移植的结合可能会诱导长期的免疫耐受活体相关器官移植。进一步的实验是必要的，以评估脾切除和脾移植的适当时机，并阐明脾移植从母亲到孩子诱导特异性免疫耐受的动物模型中，不仅使用皮肤移植，但器官移植作为第二次移植的效果。联合应用受体脾切除术和脾移植术可诱导对活体小肠移植的长期免疫耐受。