Experimental studies to develope a new treatment for the organ transplanataion by the inducing prolonged immuno tolerance and chimerism

尽管免疫抑制剂最近取得了进展，但成功的肠移植仍然需要理想的治疗方法。诱导同种异体移植物特异性耐受，而不需要延长免疫抑制或广泛的受体预处理，有望改善肠道移植的结果，减少并发症。我们的研究目的是利用大鼠模型阐明母代脾移植对诱导活体相关肠移植的长期特异性免疫耐受的影响。从我们的结果来看，我们发现移植脾脏在儿童网膜中生长良好，而不是成人，并怀疑儿童脾脏对母亲移植的脾脏起排斥作用。我们推测，切除儿童脾脏和移植一块脾脏的组合可能会诱导对活体器官移植的长期免疫耐受。在以皮肤移植和器官移植为二次移植的动物模型中，需要进一步的实验来评估脾切除和脾移植的合适时机，并明确母亲移植脾对诱导特异性免疫耐受的影响。受体脾切除术和母亲向儿童（受体）的脾移植联合可能诱导对活体肠移植的长期免疫耐受。

Despite recent developments in immunosuppressive agents, an ideal treatment is still needed for successful intestinal transplantation. The induction of specific tolerance of allograft without the need for prolong immunosupperssion or extensive recipient pretreatment will be expected to improve the result of intestinal transplantation and to reduce the complications. The aim of our study was to clarify the effects of splenotransplantation from mother to child on the induction of prolonged specific immuno-tolerance to living related intestinal transplantation using a rat model.From our results, we found that the transplanted spleen well grew up in child omentum more that adult one's and suspected that the child spleen played to reject the transplanted spleen from mother. We speculated that a combination of extirpation of child spleen and transplantation of a piece of spleen might induce prolonged immunotolerance to living-related organ transplantation. Further experiments are necessary to evaluate the proper timing of splenectomy and splenotransplantation and to clarify the effects of the transplanted spleen from mother to child on inducing specific immunotolerance in a animal model using not only skin transplantation but also organ transplantation as the second transplantation. A combination of recipient splenectomy and splenotransplantaion from mother to child (recipient) might induce prolonged immunotolerance to living-related intestinal transplantation.