A RELATIONSHIP BETWEEN PERIODONTAL DISEASE AND ORAL HOMEOSTASIS BY WHOLE SALIVA

众所周知，人类唾液免疫球蛋白和其他唾液蛋白可以防御各种口腔疾病，包括牙龈和牙周病。一些人的整个唾液含有一种35 kDa的蛋白质，其成分和功能未知。在这项研究中，我们用sds -聚丙烯酰胺凝胶电泳分析了人类全唾液，以确定35 kDa蛋白与牙周病之间是否存在关系。收集年龄22-75岁的146例患者的唾液样本，用12.5%丙烯酰胺凝胶分离，用0.25%考马西亮蓝R-250（CBB）室温染色30 min。使用凝胶扫描分析仪通过密度测定法评估单个样品中35 kDa蛋白的存在或缺失。42个个体的唾液样本中含有35 kDa的cbb染色带，而在其余104个样本中，35 kDa的条带无法识别。对所有患者进行口腔健康检查后发现，36例唾液中含有35kda蛋白的患者中有34例无病，而104例缺乏35kda蛋白的患者被诊断为患有晚期牙周病。唾液中35 kDa蛋白的存在与诊断的牙周病之间的负相关表明该蛋白可能在口腔防御中起作用。需要进一步表征该蛋白及其与口腔组织和其他唾液防御因子的相互作用，以了解其作用机制。

It is well known that human salivary immunoglobulins and other salivary proteins provide a defense against various oral disease, including gingival and periodontal disease. Whole saliva from some individuals contains a 35 kDa protein of unknown composion and function. In this study we analyzed human whole saliva by SDS-polyacrylamide gel electrophoresis to determine if a relationship exists between the 35 kDa protein and periodontal disease. Saliva samples were collected from 146 patients, 22-75 years of age, separeted on 12.5% acrylamide gels, and stained with 0.25% Coomassie Brilliant Blue R-250(CBB) for 30 min at room temperature. The presence or absence of the 35 kDa protein in individual samples was evaluated by densitometry using a gelscan analyzer. Saliva samples from 42 individuals contained a CBB-stained band at 35 kDa In contrast, in the remaining 104 samples, the 35 kDa band could not be recognized. Oral health examinations of all the patients were evaluated and revealed that 34 of hte 36 patients whose saliva contained the 35 kDa protein were disease-free, whereas the 104 patients who lacked the 35 kDa protein were diagnosed as having advanced periodontal disease. The negative correlation between the presence of the 35 kDa protein in saliva and diagnosed periodontal disease indicates that this protein may play a role in oral defense. Further characterization of this protein and its interactions with oral tissues and other salivary defense factors is needed to understand its mechanism of action.