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A RELATIONSHIP BETWEEN PERIODONTAL DISEASE AND ORAL HOMEOSTASIS BY WHOLE SALIVA

牙周疾病与全唾液口腔稳态之间的关系

基本信息

批准号：
08672077
负责人：
TAKANO Kunio
金额：
$ 1.28万
依托单位：
NAGASAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672077/
关键词：
salivary protein electrophoresis periodontitis whole saliva 35 kDa protein oral defense 唾液 35KDa 唾液腺 SDS-PAGE

项目摘要

It is well known that human salivary immunoglobulins and other salivary proteins provide a defense against various oral disease, including gingival and periodontal disease. Whole saliva from some individuals contains a 35 kDa protein of unknown composion and function. In this study we analyzed human whole saliva by SDS-polyacrylamide gel electrophoresis to determine if a relationship exists between the 35 kDa protein and periodontal disease. Saliva samples were collected from 146 patients, 22-75 years of age, separeted on 12.5% acrylamide gels, and stained with 0.25% Coomassie Brilliant Blue R-250(CBB) for 30 min at room temperature. The presence or absence of the 35 kDa protein in individual samples was evaluated by densitometry using a gelscan analyzer. Saliva samples from 42 individuals contained a CBB-stained band at 35 kDa In contrast, in the remaining 104 samples, the 35 kDa band could not be recognized. Oral health examinations of all the patients were evaluated and revealed that 34 of hte 36 patients whose saliva contained the 35 kDa protein were disease-free, whereas the 104 patients who lacked the 35 kDa protein were diagnosed as having advanced periodontal disease. The negative correlation between the presence of the 35 kDa protein in saliva and diagnosed periodontal disease indicates that this protein may play a role in oral defense. Further characterization of this protein and its interactions with oral tissues and other salivary defense factors is needed to understand its mechanism of action.

众所周知，人唾液免疫球蛋白和其他唾液蛋白提供对各种口腔疾病的防御，包括牙龈和牙周疾病。来自某些个体的全唾液含有未知组成和功能的35kDa蛋白质。在这项研究中，我们分析了人全唾液的SDS-聚丙烯酰胺凝胶电泳，以确定是否存在的关系之间的35 kDa蛋白和牙周疾病。从146名22 - 75岁患者中收集唾液样本，在12.5%丙烯酰胺凝胶上分离，并在室温下用0.25%考马斯亮蓝R-250（CBB）染色30分钟。使用凝胶扫描分析仪通过光密度测定法评价个体样品中35 kDa蛋白质的存在或不存在。来自42个个体的唾液样品在35 kDa处含有CBB染色条带，相反，在其余104个样品中，35 kDa条带不能被识别。对所有患者的口腔健康检查进行了评估，结果显示，36名唾液中含有35 kDa蛋白的患者中有34名无疾病，而104名缺乏35 kDa蛋白的患者被诊断为患有晚期牙周病。唾液中35 kDa蛋白的存在与诊断的牙周病之间的负相关性表明该蛋白可能在口腔防御中发挥作用。需要进一步表征这种蛋白质及其与口腔组织和其他唾液防御因子的相互作用，以了解其作用机制。