权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The experimental study to confirm the cerebroprotective effect of hypothermia during circulatory cessation.

该实验研究证实了循环停止期间低温的脑保护作用。

基本信息

批准号：
08671522
负责人：
MORI Atumi
金额：
$ 1.54万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671522/
关键词：
cerebral ischemia hypothermia deep hypothermic circulatory arrest cerebral protection immunohistochemistry delayd neuronal deach thoracic aortic aneurysm glia cell スナネズミ虚血性神経細胞死アストログリア GFAP

项目摘要

In the experimental study, we investigated the histopathological consequences of hippocampal CA1 pyramidal neurons as well as the glial reactions in the hippocampus after diverse periods of ischemic insults at graded hypothermia ranging from 32 to 30ﾟC.We used the gerbil forebrain ischemia model and clamped bilateral common carotid arteries for 5-120min. The morphological study was performed 7 days after ischemia or sham-operation. For evaluation of delayd neuronal death (DND), we used Cresyl violet staining and MAP2 immunoreactivity. Astroglial reactions were examined by GFAP immunoreactivity and microglial reactions were estimated by isolectin B4 histochemistry. The forebrain ischemia at 32ﾟC for 10 min and at 28ﾟC for 20min did not induce DND in the CA1 region. However, the ischemia at 32ﾟC for 20 min and at 28ﾟC for 30 min caused extensive degeneration of CA1 pyramidal neurons. Under the condition of deep hypothemia, the 60 min ischemia at 24ﾟC and 120 min ischemia at 20ﾟC which we … More re the longest duration of each temperature animals within the limitation of the animal survival in this experimental model induced no DND in CA1. The reactive change of astrocytes were observed in the entire hippocampus not only in ischemic animals with DND but also in ischemic animals without DND.In contrast, the distribution fo activated microglia was much more restricted to the CA1 region of ischemic animals with DND.The present results demonstrate the remarkable neuroprotective effect of deep hypothermia that has been used in cardiovascular surgeries. The findings also suggest that even under the hypothermic condition, glial reactions may play an important role in the process of neuronal survival and death after ischemia.We have also performed the surgeries for thoracic aortic aneurysms using this technique of deep hypothermia as the cerebroprotective strategy during total circulatory cessation, furthermore, we applied this technique to the extended resection of invasive thymoma and reported a good result. Less

在实验研究中，我们研究了在32 ~ 30ºC的分级低体温条件下，不同时间的缺血损伤对海马CA1锥体神经元的组织病理学影响以及海马胶质细胞的反应。采用沙鼠前脑缺血模型，夹持双侧颈总动脉5 ~ 120min。缺血或假手术后7 d进行形态学研究。为了评估延迟性神经元死亡（DND），我们使用甲酚紫染色和MAP2免疫反应性。用GFAP免疫反应检测星形胶质细胞反应，用分离素B4组织化学检测小胶质细胞反应。32 ~ 20min和28 ~ 20min的前脑缺血未引起CA1区DND的发生。然而，32、28、30分钟的缺血可引起CA1锥体神经元的广泛变性。在深度低体温条件下，24ºC下缺血60 min和20ºC下缺血120 min是本实验模型动物在生存限度内各温度下持续时间最长的CA1无DND。无论是缺血性DND动物，还是非缺血性DND动物，都观察到整个海马星形胶质细胞的反应性变化。相比之下，缺血DND动物活化小胶质细胞的分布更局限于CA1区。目前的研究结果表明，深度低温在心血管手术中具有显著的神经保护作用。研究结果还提示，即使在低温条件下，神经胶质反应也可能在缺血后神经元的存活和死亡过程中发挥重要作用。在全循环停止期间，我们也使用深低温技术作为脑保护策略进行了胸主动脉瘤的手术，此外，我们将该技术应用于侵袭性胸腺瘤的扩大切除，并报告了良好的结果。少