Prevention of hypoxic brain damage by in vivo transfection through the carotid artery in cirsulatory arrest

点击翻译按钮获取中文摘要

Efficient gene transfer method into the whole heart through the coronary artery with Hemagglutinating Virus of Japan liposoine this method represents a novel in vivo gene transfar technique for the heart and thus may provide a new tool for this project and research.In vivo gene transfection with heat shock protein 70 and bcl-2 enhance myocardial and liver cell tolerance to ischemia-reperfusion injury : overexpressed HSP 70 and Bcl-2 in rat hearts and livers plays a protective role in ischemia- reperfusion injury, suggesting the possibility that gene transfection with HSP 70 and bcl-2 may become a novel method for the organ protection.Gene transfar method into the brain through the carotid artery in hypothermic circulatory arrest HVJ-liposome was used for gene transfection of oligonucleotide labeled with fluorescein into the rat brain through the carotid artery. Fluorescein isothiocyanate was detected in the nuclei.In vivo gene transfection with heat shock protein 70 and bcl-2 into the brain for the protection in ischcmia-reperfusion injury : HSJ 70 and bcl-2 are transfered into the rat brain.