Role of protease on spinal cord ischemia and reperfusion

蛋白酶在脊髓缺血和再灌注中的作用

• 批准号: 08671530
• 负责人: FUJIMURA Yoshihiko
• 金额: $ 1.22万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671530/
• 关键词: Thoraco-abdominal Aneurysm; Spinal cord ischemia; Paraplegia; Protease; Calpain-inhibitor

We investigated the role of protease (Calpain) on spinal cord ischemia and reperfusion. Japanese white rabbits were anesthetized and ventilated mechanically. 4th left thoracotomy was made for clamping the descending thoracic aorta. Just before aortic clamping, calpain inhibitor-1 (10 mu mol/kg) was administered intravenously in calpain group (n=7). Control group rabbits (n=7) received no inhibitor. After 20 minutes' aortic clamping, clamp was removed and chest was closed. Neurological observation was performed for 48 hours by means of Tarlov's score. 6 of 7 rabbits in control group developed complete paraplegia and remaining rabit developed severe paralysis. In contrast, 3 of 7 rabbits in calpain group were recovered without severe paralysis and only 2 rabbits developed complete paraplegia. There was significant difference between two groups (p=0.017). 150-kD fragment of alpha-calspectin was apparantly dense in complete ischemica spine by western blotting. It suggests that calpain play important role on spinal cord ischemia and reperfusion. Histopathology also revealed the spinal cord injury in control rabbit whereas no injury in calpain group.We conclude that calpain inhibitor-1 may protect spinal cord during ischemia and reperfusion.

我们研究了蛋白酶（钙蛋白酶）对脊髓缺血和再灌注的作用。日本白兔采用机械麻醉和通气。第四次左胸廓切开术用于夹闭胸降主动脉。钙蛋白酶组(n=7)在主动脉夹闭前静脉注射钙蛋白酶抑制剂1(10μmol/kg)。对照组兔子（n=7）不接受抑制剂。主动脉夹闭20分钟后，移除夹子并关闭胸腔。通过Tarlov评分进行48小时的神经学观察。对照组7只兔子中有6只出现完全截瘫，其余兔子出现严重瘫痪。相比之下，钙蛋白酶组7只兔子中有3只康复，没有严重瘫痪，只有2只兔子出现完全截瘫。两组之间存在显着差异（p=0.017）。通过蛋白质印迹法，150-kD α-钙调蛋白片段在完全缺血的脊柱中明显致密。提示钙蛋白酶在脊髓缺血再灌注中发挥重要作用。组织病理学也显示对照兔有脊髓损伤，而钙蛋白酶组没有损伤。我们得出结论，钙蛋白酶抑制剂-1可能在缺血和再灌注过程中保护脊髓。