OPTIMUM POTASSIUM CONCENTRATION OF THE PULMONARY PRESERVATION SOLUTION FOR CULTURED PULMONARY ENDOTHELIAL AND SMOOTH MUSCLE CELLS

点击翻译按钮获取中文摘要

The optimal potassium concentration of lung preservation solution has not been investigated systematically. The purpose of this study was to evaluate the effect of solutions with different potassium concentration on cultured pulmonary artery endothelial and smooth muscle cells.A low potassium dextran solution with 4 mmol/L K+(LPD), a moderate potassium dextran solution with 60 mmoI/L K+(MPD) and a high potassium dextran solution with 100 mmol/L K (HPD) were used for preservation.The viability of those cells stored in each solution at 10ﾟC was determined by MTT assay. MTT assay absorbance values for cultured pulmonary artery endothelial cells after 24 hours of cold storage were 0.147 * 0.011(mean*standard error) in LIP, 0.125*0.013 in MPD, and 0.189 * 0.039 in HPD (P<0.05 : HPD vs LPD and MPD). MTT assay absorbance values f* cultured pulmonary artery smooth muscle cells after 24 hours of cold storage were 0.028 * 0.003 in LPD, 0.042*0.008 in MPD, and 0.053*0.008 in HPD (PK<O.05 : HPD vs LPD and MPD).From our results , we conclude that HPD is superior to LPD or MPD in 24-hour cold preservation of pulmonary artery endothelial and smooth muscle cells.