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Spinal cord demyelination with experimental continuous compression in rat

实验性连续压迫大鼠脊髓脱髓鞘

基本信息

批准号：
08671621
负责人：
IIZUKA Hideaki
金额：
$ 0.58万
依托单位：
KANAZAWA MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671621/
关键词：
compressive myelopathy demyelination axonal degeneration 実験脊髄損傷

项目摘要

To analyze the mechanism of chronic compression myelopathy in human spondylosis, ossification of ligaments, and spinal cord tumors, we tried to make a spinal cord continuous compression model in rats using uterine-cervical dilator Dilapan, which has the character of slow expanding, as compression-materials and investigated the lesions. The spine was pressured continuously with compression-material in the sublaminal epidural space at Th9 vertebral level for 1,2,3,7,14,30 and 90 days. The model was investigated morphologically, by histochemistry, immunohistochemistry, electron microscopy as well as conventional histology. The morphological data were analyzed quantiatively by morphometries.In this model, Dilapan occupied 24.2% of spinal canal in the cross sections. All rats transiently became spastic paraplegia after the placement of compression-materials, but the palsy was recovered in 80% of the rats. Histologically, at the 1st day, hemorrhagic necrosis of the spinal cord occurred, whic … More h was mostly localized in the pressured sites, i.e.posterior column. In the white matter, a spongy change appeared at 2nd day and lasted until 90th day. The spongy change was not localized at the compression site but distributed diffusely in the white matter of spinal cord, although the change was stronger in deeper parts of white matter than in superficial ones. Histochemistry and immunohistochemistry disclosed that the spongy change of white matter was associated with decrease of axon and neurofilaments, but not with demyelination. Examination of toluidine blue staining of thin sections and electron microscopy demonstrated that main change occurred in the lateral column was the degeneration of axon of myelinated fibers, such as edematous swelling, loss of axon causing myelin-tangles, and detachment from myelin sheath. These axonopathy had been continuously seen as long as 90th day. Changes of myelin, such as fragmentation, splitting, and thinning, were also seen in the lateral column but the frequency was low. Morphometry of diameters of myelinated fibers and thickness of myelin did not indicate any significant change of myelin. Furthermore, release of compression decreased the number of degenerated axons. These findings showed that continuous spinal cord compression model using Dilapan is suitable for the analysis of chronic compression myelopathy, indicating that axonopathy but demyelination may be one of the main changes in chronic compression myelopathy. Less

为探讨人类颈椎病、韧带骨化、脊髓肿瘤等慢性压迫性脊髓病的发病机制，本研究采用扩张缓慢的子宫颈扩张器Dilapan作为压迫材料，建立大鼠脊髓持续压迫模型，观察其损伤情况。在Th9节段硬膜外腔持续加压1、2、3、7、14、30、90 d。通过组织化学、免疫组化、电镜和常规组织学对模型进行形态学研究。形态计量学定量分析结果表明，在该模型中，Dilapan占椎管横截面的24.2%。所有大鼠在放置压缩材料后均出现短暂的痉挛性截瘫，但80%的大鼠麻痹恢复。组织学上，第1天脊髓发生出血性坏死， ...更多信息 h主要位于受压部位，即后柱。白色物质在第2天出现海绵状变化，并持续到第90天。海绵样改变不局限于脊髓受压部位，而是弥漫性分布于脊髓白色质，但白色质深部的海绵样改变较浅部强。组织化学和免疫组化结果显示，白色物质的海绵样改变与轴突和神经丝的减少有关，但与脱髓鞘无关。切片甲苯胺蓝染色及电镜观察表明，侧柱主要改变为有髓纤维轴突变性，如水肿肿胀、轴突缺失、髓鞘缠结及脱离髓鞘等。这些轴突病变已连续出现长达90天。侧柱也可见髓鞘断裂、分裂、变细等改变，但发生率较低。有髓纤维直径和髓鞘厚度的形态计量学检查未发现髓鞘有明显变化。此外，释放压力减少了退化轴突的数量。这些结果表明，使用Dilapan的持续脊髓压迫模型适用于慢性压迫性脊髓病的分析，表明轴突病变但脱髓鞘可能是慢性压迫性脊髓病的主要变化之一。少