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Effect of Exogenous Growth Factors on Ligament Healing

外源性生长因子对韧带愈合的影响

基本信息

批准号：
08671640
负责人：
FUKUI Naoshi
金额：
$ 1.47万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671640/
关键词：
Growth Factor Ligament Healing basic FGF GDF-5 bFGF TGF 力学試験 PDGF IGF 靱帯 procollagen mRNA

项目摘要

In the first year of this two-year research project, we evaluated the spatial and temporal expression of the growth factors during the healing process of the medial collateral ligament (MCL) in rabbits, using a technique of immunohistochemistry.Based on its results, we further studied the effect of exogenous growth factor on the healing process of ligaments. Firstly, we tried basic fibroblast growth factor (bFGF) to promote ligament healing. Using established model of delayed healing in rabbit's MCL, bFGF was administered using fibrin glue as a vehicle. As the result, exogenous bFGF promoted the healing of MCL to some extent, however, dosage was critical and excessive dose of bFGF might exert unfavorable effects. These results were published in Revue du Rhumatisme English Edition.As the next step of our project, the effect of combined administration of bFGF and TGF-beta1 was studied. This study was, however, discontinued in its preliminary steps because the results were not promising.Subsequently, we investigated the effect of growth and differentiation factor-5 (GDF-5) on ligament healing in rats. This factor is a member of TGF-beta super family, and is reported to promote ectopic formation of ligamet-like tissue. As the results of our study, this factor promotes ligament healing, based on the macroscopic, histological, biomechanical, and biochemical evaluations. This study is on its halfway now and will be completed within several months.

本研究在第一年采用免疫组织化学方法观察了家兔内侧副韧带（MCL）愈合过程中生长因子的时空表达，并在此基础上进一步研究了外源性生长因子对韧带愈合过程的影响。首先，我们尝试碱性成纤维细胞生长因子（bFGF）促进韧带愈合。利用建立的兔MCL延迟愈合模型，以纤维蛋白胶为载体给予bFGF。结果表明，外源性bFGF对MCL的愈合有一定的促进作用，但剂量有一定的限制，过量可能产生不良影响。这些结果发表在Revue du Rhumatisme英文版上。作为我们项目的下一步，我们研究了bFGF和TGF-β 1联合给药的效果。然而，这项研究是在其初步步骤中断，因为结果并不乐观。随后，我们研究了生长和分化因子-5（GDF-5）对大鼠韧带愈合的影响。该因子是TGF-β超家族的成员，据报道可促进韧带样组织的异位形成。作为我们研究的结果，基于宏观、组织学、生物力学和生物化学评价，该因子促进韧带愈合。这项研究现已进行了一半，并将在几个月内完成。